小麦品种磷营养特性的类型分析及其年度间稳定性的研究

利用大田试验对１０７份小麦品种的磷营养特性进行了鉴定。结果表明,各品种按其生产力水平和对土壤磷的利用效率可划分为,高产高效、高产中效、高产低效、中产高效、中产中效、中产低效、低产高效以及低产中效８种类型,其出现频率分别为６．５％、３６．５％、８．４％、８．４％、１５％、４．６％、５．６％和１．９％。小麦品种耐低磷特性在不同年度间相当稳定,可以通过土培试验与大田试验相结合的方法进一步筛选出最稳定的“     

黑麦1R染色体特异性PCR引物的分子证据

Based on the differences of rRNA intergenic sequences between wheat ( Triticum aestivum L. ) and rye ( Secale cereale L. ), rye specific primer set NOR-R1 was synthesized according to Koebner' design. PCR analyses were carried out on different DNA substrates of common wheat and its relatives such as Agropyron elongataum (Host) Beauv., Haynaldia villosa Shur. and Hordeum vulgare L. The results confirmed that NOR-R1 primer set is specific to rye. It was found that PCR using DNAs from wheat materials containing 1R chromosome resulted in the specific amplification products of rye, whereas no amplification product was detected in PCR when using DNAs with other rye chromosomes. FISH (Fluorescent in situ Hybridization) further revealed that the binding sites for the primer set NOR-R1 were only on nucleolar organizing region of chromosome 1R. These results indicated that the primer set NOR-R1 provides a useful means for molecular tagging of rye chromosomes 1 R in wheat genetic background.