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利用完整细胞不对称合成R-苯乙醇胺的研究 总被引:2,自引:0,他引:2
Effects of various factors on asymmetric synthesis of R-phenylaminoethanol from aminoacetophenone by the whole cells of Arachnia sp. P163 producing alcohol dehydrogenase for phenylethanol amine was investigated. It found that, although the reduction was inhibited by the substrate and the product, but it has the very high stereoselectivity. The reduction was normaly carried out with 2% glucose for reproduction of coenzyme in the reaction system without oxygen. The conversion yield and ee value of the product achieved 65% and 100%, respectively. 相似文献
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产苯乙醇胺醇脱氢酶菌种筛选与发酵条件研究 总被引:2,自引:0,他引:2
A Arachnia sp.P163 producing alcohol dehydrogenase which is able to reduce aminoacetophenone to R\|1\|phenyl\|2\|aminoethanol was obtained from soil and cultures.The maximum activity of enzyme was produced by the LB medium containing 1% sodium citrate and peptone,0 1% phenylaminoethanol as inducer at 30℃ for 48 hs. 相似文献
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