多倍体麦类作物Wx蛋白检测的SDS-PAGE方法

本文通过对小麦蜡质蛋白的实验和研究,详细论述了蜡质蛋白的特性、提取 原理、电泳原理和检验原理。对多倍体麦类作物Wx蛋白亚基的检测,提出了应用效果良好的电泳系统,并就提取方法、凝胶配方和操作步骤进行了描述。本文还列出了部分麦类作物蜡质蛋白亚基基本模式示意图和实验例证,具有很好的参考价值。 Abstract:The waxy protein property,principles of its extraction,electrophoresis and detection were discussed in detail in this paper.According to the detection of polyploid wheat waxy subunits,an effective electrophoresis system was given with the extraction method,gel composition and operating steps listed.The basic iso-form patterns of wheat and related species as well as an application example were also showed in the paper.It is recommended that this paper is valuable both as reference and in application.     

组织培养创造抗白粉病小麦-簇毛麦染色体易位及分子标记辅助选择

对175株普通小麦与6D/6V代换系杂种当代幼胚培养再生植株进行谷草转氨酶同工酶分析,其中来自杂交组合遗4095×RW15（6D／6V代换系）的2个植株（编号分别为98R149和98R159）GOT—V