湖北后河国家级自然保护区野生花卉资源

后河国家级自然保护区位于鄂西南湘鄂边界山区,野生花卉资源丰富,有"三峡绿宝石"之美誉.笔者分析了保护区的野生花卉资源现状及其开发利用.重点介绍了各类花卉的生物特性和利用的方法.     

芫花的花粉形态观察及生活力和萌发率分析

Pollen morphology of Daphne genkwa Sieb.et Zucc.was observed by scanning electron microscope(SEM),and its viability and germination rate were measured by TTC and sucrose in vitro culture methods.The results show that pollen is stenopalynous type with a diameter of 15.6-21.6 μm.Per pollen has 10-16 apertures which is irregular circular with a diameter of 1.4-2.0 μm.Surface ornamentation of pollen is rough reticulate pattern which is circular polygon(tetragon-heptagon,mostly pentagon-hexagon).Pollen viability is 51% by TTC.Sucrose of different concentrations has a significant effect on pollen germination rate during pollen culture.And pollen germination rate is the highest with a percentage of 27.0% in medium containing 50 g·L-1 sucrose,while pollen could not germinate in medium containing sucrose over 250 g·L-1.Otherwise,there is the phenomenon of "multi-aperture germination" during pollen germinating.     

新生大鼠肺成纤维细胞的原代培养改良法及细胞鉴定

探讨新生大鼠肺成纤维细胞原代培养的改良方法及细胞鉴定。用胰酶消化组织块结合的方法提取新生大鼠肺成纤维细胞,并纯化细胞,对肺成纤维细胞进行形态学观察,用HE染色及免疫组化染色法对细胞进行鉴定,并用MTT法测定细胞生长曲线。倒置相差显微镜下观察选用改良法获得的细胞,3 d后可见组织块周边有少许细胞,5 d后组织块周围有大量细胞爬出,生长迅速,10 d接近融合。经改良后的方法纯化细胞,细胞活性状态较好的为3~5代,5代以后的细胞增殖能力下降。对第3代肺成纤维细胞进行HE染色,镜下可见形态典型的成纤维细胞,免疫组化结果显示波形蛋白(Vi-mentin)阳性表达,细胞角蛋白(cytokeratin)阴性表达。MTT法检测第3代细胞于3~5 d处于对数生长期。胰酶消化组织块结合法是一种可靠快速的肺成纤维细胞分离纯化的培养方法,使用这种方法可得到具有典型形态特征且活性较好的肺成纤维细胞,初学者容易掌握。