Inhibition of the growth of human hepatoma cell line both in vitro and in vivo by transducing CKI gene p21~(WAF-1) with GE7 targeting gene delivery system

The EGF receptor-mediated targeting gene delivery system GE7 was used to transduce exogenous gene pCEP-p21WAF-1 into human hepatocellular carcinoma cell both in vitro and in vivo. After in vitro transduction of the exogenous gene, the growth of the cell lines SMMC-7721 and BEL-7402 was significantly inhibited compared with the control. On day 8 the inhibition rates of the above cell lines reached 56.0% and 66.7%, respectively. The in vivo experiment showed that the growth of human hepatoma transplanted in nude mice injected with GE7 gene delivery system subcutaneously once a week for 3 weeks was remarkably inhibited compared with that of untrans-fected control. The average tumor weight of the experiment group was (0.083 ?0.043) g, while that of the control group was (0.28110.173) g. The difference is significant (P<0.05). It was indicated that GE7 gene delivery system could efficiently transduce exogenous gene pCEP-p21WAF-1 into hepatoma cell with high EGF receptor expression, and inhibit the cell growt     

靶向性非病毒载体介导p21WAF-1基因对肝癌细胞的抑制作用

利用研制开发的靶向性非病毒载体GE7基因导入系统转导外源基因pCEP-p21WAF-1,检验其介导p21WAF-1基因进入肝癌细胞后,在体内外抑制肝癌细胞的生长的效率.体外实验证明,导入外源基因后,SMMC-7721和BEL-7402细胞的生长受到抑制,第8天抑制率分别达到56%和66.7%.而体内实验结果是,荷SMMC-7721裸鼠皮下瘤周注射该基因导入系统,转导pCEP-p21WAF-1 3周后,实验组肿瘤的重量(平均为(0.083 ± 0.043)g)与对照组(平均为(0.281± 0.173) g)相比明显减小,且在统计学上有显著差别(P<0.05).以上结果表明,GE7基因导入系统可以介导外源基因pCEP-p21WAF-1进入细胞内,并在体内外抑制肝癌细胞的生长.