我国一株鹦鹉病毒的鉴定及其生化特性

１９９５ 年作者从湖北濒死鹦鹉体内分离到一株新病毒,经鹦鹉胚成纤维细胞盲传四代过渡到鸡胚成纤维细胞,再传至第五代出现细胞病变。适应细胞的病毒纯化后,经 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 分析,发现病毒粒子结构蛋白由８ 条多肽组成,分子量范围在１４ ．５ ～６０ ｋ Ｄ 之间。病毒的酸碱氨基酸之比为２ ．５１ ,富含 Ａｓｐ 、 Ｃｌｕ 和 Ｌｅｕ ,而 Ａｒｇ 和 Ｍｅｔ 含量较少。病毒核酸为双链 Ｄ Ｎ Ａ,存在超螺旋型、环型和线型三种形态, Ｇ＋ Ｃ 的含量为４５ ％ 。经限制性内切酶分析,核酸的分子量为３ ．３ ×１０６ Ｄ。根据病毒分类的标准确认为澳州长尾小鹦鹉幼雏病病毒,应归属于乳多空病毒科多瘤病毒属。     

我国一株鹦鹉病毒的鉴定及其生化特性

A strain of new virus was isolated from fledgling budgerigars by authors in 1995. Cytopathic effect became visible after the isolate was passaged 4 generations on budgerigar embryo fibroblast and 5 generations on Chicken embryo fibroblast. The SDS-PAGE proved that viral capsid was composed of eight polypeptides with molecular weight from 60 000 to 14 500 D. The protein was rich in Asp, Glu and Leu, but comparatively poorer in Arg and Met. The ratio of acidic amino acid to basic amino acid was 1∶2.51. The nucleic acid was dsDNA and existed as supercoiled circular, relaxed circular and linear molecules. G+C of viral DNA was 45%. The molecular weight of DNA was 3.3×10⁶D by restriction endonuclease analysis. According to standards of virus classification, it is proved that the isolate was budgerigar fledgling disease virus which had placed into the Polyomavirus genus of Papovaviridae.