TT病毒ORF1基因在大肠杆菌中的表达及TTV酶免疫诊断方法的初步建立

为建立免疫学检测TT病毒抗体的方法,我们表达了硫氧还蛋白与TTV ORF1 497aa～770aa段融合蛋白,采用免疫印迹分析表达产物的抗原性,并以表达产物为抗原检测TTV抗体,结合TTV DNA检测结果分析其抗原特异性。     

严重急性呼吸综合征(SARS)冠状病毒抗体的检测及其临床意义

To investigate the k inetics of antibody to SARS coronavirus in SARS patients and its clinical implication,ELISA was used to dete ct antibody to SARS coronavirus(SA RS CoV),RT-PCR was used to detect the SARS CoV RNA and,besides,the C D+4 and CD+8 T cells in peripheral blood of SARS patients and healthy controls were assayed by flowcytom etryThe results showed that SARS CoV IgM were first detected from da y 7 to day 47 after SARS onset,wit h average at day 193±101SARS Co V IgG were first detected from day 4 to day 47 after SARS onset,with average at day 207±101,and the p roduction of SARS CoV IgG was corr elated with CD+4 T cell number(P<005),but had no relationship with SARS CoV RNAMost SARS patients pr oduced SARS CoV antibody,IgM produ ced almost at the same time wit h IgGSARS CoV IgG is a protective antibody against SARS CoV and the titer of IgG may be used as an ind ex indicating the specific immunit y production in SARS patients     

五株登革病毒的分离鉴定及E基因序列分析

登革病毒作为一种重要的蚊媒传播病毒,威胁全球人类健康,及时甚至实时了解病毒的变异和流行病学规律,对科学防控登革热具有重要意义.因此,本研究利用广东省深圳市发现的登革热患者血清进行病毒分离、鉴定及囊膜蛋白E基因的扩增、测序及生物信息学分析病毒E基因进化特点.结果显示,应用Vero和C6/36细胞培养法从登革热病患血清中分离获得5株登革病毒,利用MEGA7软件对囊膜蛋白E的核苷酸和氨基酸序列进行分析显示,5株均属血清Ⅱ型登革病毒,其中输入性SZ926株和本地株SZ38株同源性为99.5％,均与泰国16681经典毒株关系密切,可能衍生于同一祖先;SZ868株和SZ871株为境外输入性病患分离株,两株病毒核苷酸同源性达到99.9％,说明两株病毒可能源于同一毒株;SZ29株与新几内亚的New Guinea C毒株同源性为99.8％,显示出进化保守、稳定传播的特点.基因型分析显示,SZ926和SZ38为基因型Ⅲ型,SZ868和SZ871为基因型Ⅳ型,SZ29为基因型Ⅰ型.本研究成功分离获得5株血清Ⅱ型登革病毒,分属于3个基因型,且不同基因型的E基因整体变异较低,而且我国存在多种基因型感染的潜在威胁,必须加强防范意识和研究力度.