脯氨酸转运相关基因的研究进展

作为植物中普遍存在的一种逆境适应机制, 脯氨酸积累一直被认为是其合成和降解调控的结果。然而越来越多的研究表明, 脯氨酸转运也可能在其积累过程中起重要作用。在植物中, 有多个氨基酸转运蛋白家族, 如氨基酸通透酶家族(AAPs)、赖氨酸组氨酸转运蛋白家族(LHTs)和脯氨酸转运蛋白家族(ProTs)参与脯氨酸在各个器官间的运输。该文对参与脯氨酸运输的基因家族成员的表达模式、生理功能及表达调控进行了综述, 以期为脯氨酸运输与积累在植物抗逆方面的研究提供参考。     

农杆菌介导的Intron－GUS嵌合基因转入花椰菜获得转基因植株

农杆菌介导的Ｉｎｔｒｏｎ－ＧＵＳ嵌合基因转入花椰菜获得转基因植株陈晓邦,华学军,黄其满,范云六（中国农业科学院生物技术研究中心分子生物学研究室,北京１０００８１）ＴＲＡＮＳＧＥＮＩＣＰＬＡＮＴＳＯＢＴＡＩＮＥＤＢＹＡＴＵＭＥＦＡＣＩＥＮＳ－ＭＥＤＩＡ...     

苏云金芽孢杆菌以色列亚种130kd杀蚊蛋白基因的...

The location of 130kd mosquitocidal protein gene of Bti 4Q5 strain on its 75Md plasmid was confirmed by southern hybridization using a 18-base oligonucleotide probe. The crystal protein containing the component of 130kd toxic protein was purified. The crystal protein exhibiting the mosquitocidal activity against larvae of Aedes aegypti was shown by bioassay. The purified 75Md plasmid DNA of Bti 4Q5 strain was completely digested with HindIII restriction enzyme, ligated with the vector pUC18 and transformed into the recipient cells of E. coli TG1. From Apr transformants, four clones with HindIII restriction fragment inserts highly homologous to the 18-base oligonucleotide probe were obtained by in situ hybridization and southern hybridization. The 5.2kb HindIII restriction fragment insert was obtained in clone pFH2 and clone pFH4, and 2.3kb HindIII restriction fragment insert in clone pFH1 and pFH3. For pFH2 and pFH4, the 5.2kb fragment was inserted in pUC18 in opposite orientation. It contained 130kd mosquitocidal protein gene (type I) identified by restriction enzyme map analysis. The 2.3kb HindIII fragment insert in other two clones (pFH1 and pFH3) harbored a part of the type II mosquitocidal protein gene which can be used as a probe for cloning of the type II mosquitocidal protein gene.     

苏云金芽孢杆菌以色列亚种130kd杀蚊蛋白基因的克隆与鉴定

以人工合成的130kd杀虫蛋白基因的18—base序列为探针,通过SoutherD分子杂交,验证了在Bacillus thuringiensis var.israelensis 4Q5菌株75Md质粒上含有130kd杀蚊蛋白基因,并且证明了提纯的晶体蛋白具有杀蚊幼虫活性。对该质粒进行HindIII完全酶切,以pUCl8为载体,以E．Coli TG1为受体,得到四个与探针有强杂交信号的阳性克隆,其中两个(pFH2,PFH4)含有5．2kb HindIII插入片段,包含第一类130kd杀蚊蛋白基因;另两个(pFHI,pFH3)含有2．3kb HindIII插入片段,包含第二类13 0kd杀蚊蛋白基因的3'部分。pFH2和pFH4中,第一类130kd杀蚊基因的插入方位不同。