多浪羊MHC-DRB3基因座的PCR-RFLP多态性分析

主要组织相容性复合体（MHC）是由紧密连锁的高度多态的基因位点所组成的染色体上的一个遗传区域,它在动物机体的免疫系统中发挥着非常重要的作用。应用PCR-RFLP技术首次对多浪羊的MHC-DRB3的外显子2进行分子遗传多态性检测与分析。结果显示,多浪羊MHC-DRB3基因的外显子2在TaqⅠ、PstⅠ和HaeⅢ酶切位点存在多态,其酶切位点分别由2、2和6种共显性等位基因控制。综合3种酶切结果,本实验研究在多浪羊中检测到了DRB3基因的24种等位基因。 Abstract:MHC is a chromosomal region consisting of a group of closely linked loci which are highly polymorphic,and plays a central role in the immune system.The restrictive polymorphism of MHC-DRB3 exon2 in Dolang sheep was Analyzed by PCR-RFLPs.The results revealed extensive polymorphisms 2,2 and 6 RFLP types of PCR products were found with enzymes TaqⅠ,PstⅠ and HaeⅢ respectively.Considering all restrictive pattern,24 alleles for DRB3 locus were found in Dolang sheep.