首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   18篇
  国内免费   2篇
  完全免费   3篇
  2004年   2篇
  1995年   3篇
  1992年   4篇
  1991年   3篇
  1989年   1篇
  1987年   2篇
  1986年   3篇
  1985年   1篇
  1983年   1篇
  1981年   1篇
  1979年   1篇
  1978年   1篇
排序方式: 共有23条查询结果,搜索用时 31 毫秒
1.
2.
本文报道CBSF是一种运用于骨髓瘤细胞、淋巴细胞杂交瘤细胞的无血清培养液。我们成功地培养了几株骨髓瘤和杂交瘤细胞,它们能长期在其中生长繁殖传代,并保持杂交瘤细胞能持续分泌抗原特异的抗体。  相似文献
3.
体外诱发抗体生成过程中淋巴细胞的变化   总被引:1,自引:0,他引:1  
An in vitro system for induction of antibody responses of human cells has been established in our lab. B cell enriched fractions from excised human tonsils or trauma spleen were cultured for 7-14 days with tetanus toxoid or HBsAg vaccine with or without human T cell conditioned medium (C. M.) or a mixture of low concentrations of PWM and LPS (MTG). Positive antibody responses could be detected in cultures. Cells taken from different culture periods were subjected to FACS analysis in order to expound cellular changes during antibody induction periods so as to improve the in vitro antibody induction system. The results were described as follows: 1. Variations in total percentages of T cells during culturing periods seemed to be related its initial percentages. Cells with bigger initial percentages tended to decrease first and finally maintained at about 30%. While cells with smaller initial percentages tended to increase and finally also maintained at 30%. 2. CD4+ Th cells and CD8+ Ts cells from tonsils and spleen behaved somewhat differently. In tonsil cell cultures the percentages of CD4+ cells were often bigger than the percentages of CD8+ cells throughout the culture period. However, the inverted proportions of CD4+/CD8+ were shown in spleen cell cultures, especially in the culture with C. M. The possible relationships between the variations in CD4+/CD8+ proportions described as above and the intensities of antibody responses were discussed. Additionally, adding 1-Leucine-Methyl Ester showed no effects either on CD8+ or CD4+ cell percentages. 3. B cell (SIg+) percentages in both tonsil and spleen cultures were quite stable throughout the culture period, about 60% of total cells. CD19, a marker of B cell, was only present in part of the cultured SIg+ cells. The significance of the variations in CD19+, SIg+ cells was unclear. CD5+ B cells were known as cells secreting autoantibodies. Our results showed that these cells consistently maintained a relatively low percentage in the whole antibody induction period. 4. The reasonableness standard we used for "gating" in FACS analysis was discussed.  相似文献
4.
小鼠对天花粉蛋白体内及体外免疫应答的基本特点   总被引:1,自引:0,他引:1  
C 57 BL/6 J小鼠在应用弗氏全佐剂或铝矾为佐剂结合天花粉蛋白免疫后都能引起抗原特异的IgE类抗体的反应以及其它Ig类抗体的反应;IgE的滴度和总的抗天花粉蛋白抗体的滴度的动态变化趋势基本一致,但并不完全同步。IgE类上升得较IgG等类抗体为慢,但上升的速度要快。脾和肠系膜淋巴结细胞分泌抗体的动态变化也和血清并不完全一致。天花粉蛋白在一定浓度下能抑制小鼠T淋巴细胞在体外的抗原特异的增殖反应和ConA反应。这抑制并不限于增殖过程的启动。补充外源性的IL-2也不能消除这抑制作用。天花粉蛋白加热变性后丧失了对小鼠淋巴细胞的毒性,并能引起经未变性天花粉蛋白体内致敏的小鼠的T淋巴细胞在体外的明显增殖。应用微量的天花粉蛋白为抗原,以及少量的无凝集素的conA条件培液等能在体外诱发致敏的B淋巴细胞产生二次抗体应答。  相似文献
5.
以IL-8免疫的BALB/C小鼠脾细胞与Sp2/0或653小鼠骨髓瘤细胞融合构建了淋巴细胞杂交瘤克隆I8-S2和I8-63。ELISA叠加试验(ELISA Additivity Test)表明这两杂交瘤克隆分泌的单抗分别识别IL-8分子的不同表位。IL-8能激活人颗粒细胞,引起细胞内Ca^2+浓度(「Ca^2+」)上升。通过流式细胞仪分析「Ca^2+」的变化,发现两个克隆单抗对IL-8激活细胞的活  相似文献
6.
The paper described a method of quantitative stability analysis of antibody production by hybridoma clones with flow cytometry (FACS). Through FACS analysis the frequency of antibody-negative variants was measured. The rate of generation of antibody-negative variants/cell/generation (mu) could be calculated through Luria-Delbrück fluctuation analysis. The results showed that the value of mu was correlated to the antibody production of hybridoma clones: the larger was the mu value, the more unstable was the antibody production by the hybridoma clones. The experimental results suggested that the clone was stable if its mu value was on the magnitude of 10(-3), while the clone was not stable if its mu value was on the magnitude of 10(-2). According to requirements of single cell for FACS analysis and presence of a variety of methods for dispersing cell aggregates to single cells, the authors regard that this method is fully suitable for analysis of cells in suspension or even in the form of aggregate.  相似文献
7.
糖皮质激素受体 (GR)为一种特异性配体诱导的转录因子在机体发育、生理及行为等过程中发挥重要作用 .这一过程往往受到转录协同调节因子的控制 ,包括协同抑制因子或协同激活因子的作用 .克隆了人源双跨膜受体分子 ,并命名为hCDC5 0 .该分子编码区有 10 5 6bp ,与鼠源基因mCDC5 0在核苷酸水平有 90 %一致性 .将hCDC5 0蛋白的 16 4~ 317编码序列与GST融合构建原核表达质粒进行重组蛋白的表达与纯化并制备多抗 .用二维双向扩散检测抗体为阳性 ,抗原的滴度为12 5 μg/ml.体外翻译研究显示该基因编码蛋白约为 4 0kD ,与Western检测在动物细胞中的表达一致 .用hCDC5 0真核表达质粒与GR萤光报告系统共转染COS 7细胞显示 ,hCDC5 0对GR信号转导有明显的抑制作用 ,其对GR信号通路的抑制作用达 3~ 4倍以上 .hCDC5 0可能为一个新的协同抑制因子 ,在抑制GR的转录激活过程中起作用  相似文献
8.
以IL-8免疫的BALB/C小鼠脾细胞与Sp2/0或653小鼠骨髓瘤细胞融合构建了淋巴细胞杂交瘤克隆I8-S2和I8-63。ELISA叠加试验(ELISA Additivity Test)表明这两杂交瘤克隆分泌的单抗分别识别IL-8分子的不同表位。IL-8能激活人颗粒细胞,引起细胞内Ca~(2 )浓度([Ca~(2 )]_i)上升。通过流式细胞仪分析[Ca~(2 )]_i的变化,发现两个克隆单抗对IL8激活细胞的活力具有不同的中和作用。克隆I8-S2具有很强的中和作用,而克隆18-63则不然。上述结果提示IL-8的激活细胞活力局限于该分子的某表位。  相似文献
9.
我们应用破伤风类毒素体外免疫的人扁桃体细胞和人-鼠异源骨髓瘤RF 系细胞进行融合,从中筛选到一株杂交瘤细胞89112—50,并通过克隆化筛选获得了两个亚克隆,其分泌的抗体是抗原特异的,和三种抗原(OVA、TC-S、FYG)都不交叉,分泌抗体的功能也比较稳定,在培养瓶内连续扩增传代13次后,仍维持相当高的抗体分泌能力,在常规传代培养过程中所收集的培养物上清液中抗体的含量平均为69.6μg/ml。  相似文献
10.
用FACS分析和Luria-Delbrǔck方程计算淋巴细胞杂交瘤株细胞丧失抗体生成功能的突变机率(μ),以评估杂交瘤的稳定性。结果表明μ值大,则杂交瘤不稳定,易丧失抗体生成功能,反之则杂交瘤比较稳定。实验证明所建立的方法能反映杂交瘤稳定性的程度,具有实验少人为主观因素干扰,统计意义强,适用范围广等优点。  相似文献
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号