费氏中华根瘤菌042BS结瘤调节基因的克隆及功能检测

费氏中华根瘤菌 (Sinorhizobiumfredii) 0 4 2BS可以在大豆和苜蓿上结瘤。用费氏中华根瘤菌USDA2 5 7的nodD1和nodD2基因分别作为探针 ,与 0 4 2BS总DNA进行Southern杂交 ,发现其DNA经EcoRI酶切后分别在 3 0kb和 6 0kb处各有一条阳性带。回收这两条阳性带附近的DNA片段 ,建立部分基因文库 ,克隆到带有nodD1基因的 3 0kb片段 ,以及带有nodD2基因的 6 0kb片段。对nodD1和nodD2进行序列分析 ,结果表明 0 4 2BS的nodD1与费氏中华根瘤菌根瘤菌USDA2 5 7和USDA1 91的同源性高达 99% ,而nodD2与USDA2 5 7的同源性为1 0 0 %。再将nodD1的片段克隆到pBBRIMCS 5载体上 ,导入豌豆根瘤菌蚕豆生物变种 (Rhi zobiumleguminosarumbv.viciae)LPR5 0 5 4中进行功能检测 ,显示 0 4 2BS的nodD1均可被大豆分泌的类黄酮物质染料木黄酮以及苜蓿分泌的类黄酮物质毛地黄黄酮所诱导     

组织型纤溶酶原激活剂的纯化制备

简述了用于大规模生产组织型纤溶酶原激活剂(tPA)的重组动物细胞及其培养工艺。从重组tPA的大规模、快速纯化的角度考虑,对tPA的纯化制备方法进行了简要评述。     

The mechanism supplying acetyl-CoA for terpene biosynthesis in sweet potato with black rot: Incorporation of acetate-2-14C, pyruvate-3-14C and citrate-2,4-14C into ipomeamarone

Silica gel thin layer chromatography showed that acetate-2-¹⁴C,pyruvate-3-¹⁴C and citrate-2,4-¹⁴C were incorporated into ipomeamaronein sweet potato root tissues infected by Ceratocystis fimbriata.Rates of incorporation of ¹⁴C, from these 3 substances, intothe CHCl³-CH₃OH-soluble lipid fraction and ipomeamarone wereof the followingder: acetate > pyruvate > citrate ¹This paper constitutes Part 82 of the Phytopathological Chemistryof Sweet Potato with Black Rot and Injury (Received December 11, 1969; )     

实夜蛾属二近缘种对寄主植物次生物质的反应:次生物质对幼虫生长和食物利用的影响

实夜蛾属(Heliothis)的棉铃虫(H. armigcra)和烟青虫(H. assulta)是近缘种,幼虫期取食多种相同的植物,其中含有不同的次生物质.本项工作是在人工饲料中分别加入0.5%的烟碱、番茄苷、棉子酚、丹宁酸等饲养早期六龄的幼虫,测定它们对生长和食物利用的影响.结果表明这些次生物质对两种幼虫有不同的作用:烟碱对棉铃虫没有明显影响,但对烟青虫的取食却有一定的刺激作用.丹宁酸、棉子酚、番茄苷可抑制两种幼虫的生长,而以番茄苷抑制烟青虫的生长最为显著.番茄苷主要通过抑制取食来影响幼虫的生长,而丹宁酸和棉子酚则具有降低消化率的作用.通过次生物质对这两种幼虫效应的比较可知,棉铃虫有较大的忍耐力.     

Laboratory and field observations on the scuticociliate Histiobalantium from the pelagic zone of Lake Constance, FRG

Histiobalantium sp. was found regularly in the pelagic zoneof Lake Constance, FRG, over five annual cycles. Maxima of upto 6400 cells l^–1 were recorded in late summer, with similarnumbers in the 0–8 and 8–20 m depth intervals. Onan annual average, the population accounted for 10–17%of the total biomass of planktonic ciliates. In the laboratory,Histiobalantium grew well on a diet of the cryptophyte Rhodomonassp. Maximum growth rates obtained in batch cultures were 0.21and 0.33 day^–11 at 9 and 18°C, respectively. In situexperiments using diffusion chambers yielded positive growthrates in autumn and winter. The highest values recorded at theambient temperatures 5, 14 and 17°C were 0.17, 0.32 and0.40 day^–1, respectively. Comparing these results withthe different seasonal distributions and higher measured growthrates of other ciliates from Lake Constance, we conclude thatHistiobalantium is a superior competitor at relatively low algalfood concentrations. ²Present address: Fisheries & Oceans Canada, 4160 MarineDrive, West Vancouver, BC, V7V 1N6, Canada     

Short- and Long-Term Effects of Salinity on Leaf Growth in Rice (Oryza sativa L.)

Addition of 50 mM NaCl to Oryza sativa L. had little effectupon the time of leaf initiation, but leaf mortality prior tothe normal phase of senescence was increased and the onset ofsenescence was advanced. There was no significant effect uponthe day-to-day pattern of growth, nor upon the ultimate length,of leaves that were developing at the time of, or shortly after,salinization with 50 mM NaCI. Leaves that developed after prolongedexposure of the plants to salinity were shorter. Addition ofNaCl, KC₁ or mannitol to the root medium brought about a cessationof leaf elongation within one minute. Growth at a reduced raterestarted abruptly after a lag period that depended upon theexternal concentration. Elongation rate recovered its originalvalue within 24 h after exposure to 50 mM NaCl, though not athigher concentrations. Addition of NaCl at concentrations upto 100 mM elicited no short-term effects upon photosyntheticgas exchange. Na uptake contributed to osmotic adjustment ofthe growing zone. When plants were rapidly exposed to 50 mMNaCl, no change in turgor pressure was detectable in the growingzone with the resolution of the miniature pressure probe used(about 70 kPa). It is concluded that the initial growth reductionin rice caused by salinization is due to a limitation of watersupply. A clear distinction is made between the initial effectsof low salinity which are recoverable and the long-term effectswhich result from the accumulation of salt within expanded leaves. Key words: Leaf elongation, gas exchange, photosynthesis, water relations     

氢化可的松高产菌株新月弯孢霉的选育*

应用酮康唑抗性筛选法,将经过紫外线诱变处理的甾体11β-羟基化菌株——新月弯孢霉的原生质体倾注在含有酮康唑最小抑制浓度(10μmol/L)的再生培养基平板上,再生出136株酮康唑抗性突变株。其中氢化可的松转化率高于出发菌株的有14株,正向突变率达到10．3％,同时获得了氢化可的松转化率为出发菌株1．42倍的遗传稳定突变株KA-91。     

一株耐高温高盐亚硝化单胞菌特性的初步研究

从某污水处理站高温高盐外排水中分离出一株严格自养的特殊亚硝化单胞菌。它为革兰氏染色阴性,不产芽孢,细胞椭球形或短杆状,呈单个排列或排列成圆形、直线形,细胞大小为（0.7～0.9)μm×（1.2～1.8)μm。在扫描电镜下发现单个或多个菌体被绒毛状不明物质包裹。该菌株能将氨氮氧化成亚硝酸盐,导致体系中的总氮减少,但是氨氮的减少与亚硝酸盐的增加并不能形成对应,而体系中几乎检测不到硝酸盐氮。在分离菌株50℃培养12 d时,约有10％的NH＋4N转变为NO-2N,15％的NH＋4N未发生转变,剩下的75％的NH＋4N被去除,包括挥发掉的17％的NH＋4N。气相色谱分析表明,菌株培养过程产生气体中氮气含量较对照气体（室内空气）增加了3.5％。     

Gathering Hopewell: Society, Ritual, and Ritual Interaction

Gathering Hopewell: Society, Ritual, and Ritual Interaction . Barbara Y. Butler. and D. Troy Case, eds. New York: Kluwer Academic/Plenum Publishers, 2005. 807 pp.