从广州市散发性脑炎病人血清中查出雪鞋野兔病毒抗体升高

近几年来夏季,在我国南方一些省市发现一些散发性病毒性脑炎病例,主要是儿童。他们的流行性乙型脑炎抗体阴性。病因不明。 为了研究本病的病因,于1983年4月至10月,我们在广州市儿童医院收集了34例散发性脑炎病人的双份血清,15例其它病种(如百日咳、心肌炎、钩端螺旋体脑炎、多发性神经根     

Aliphatic nitro-compounds in Astragalus canadensis

A reinvestigation of the alphatic nitro-compounds in Astragalus canadensis resulted in the identification of two new esters of glucose with 3-nitropropanoic acid and 5-oxotetrahydrofuran-3-acetic acid, together with six known conjugates of 3-nitropropanoic acid. ¹H and ¹³CNMR data are reported for the new compounds.     

7种铁线莲的染色体研究

本文描述了我国产毛茛科铁线莲属的7个种的染色体数目和形态。其核型公式分别为:芹叶铁线莲和宽芹叶铁线莲为2n(2x)=16=10m+6st(2SAT);黄花铁线莲和棉团铁线莲及山木通为2n(2x)=10m+4st+2t(2SAT);圆锥铁线莲为2n(2x)=32=20m+8st(2SAT)+4t(2SAT);吴兴铁线莲为2n(4x)=32=20m+6st(4SAT)+6t(4SAT);本文还讨论了一些种的分类问题。     

Separation and Purification of Antioxidant Peptides from Enzymatically Prepared Scorpion (Buthus martensii Karsch) Protein Hydrolysates

International Journal of Peptide Research and Therapeutics - The purpose of this study was to separate and purify antioxidant peptides from the scorpion (Buthus martensii Karsch) protein...     

广西弄岗五桠果叶木姜子群落木质藤本空间格局及其与树种间的关系

木质藤本是生物多样性的重要组成,木质藤本通过影响支持木进而影响群落的结构和功能,但在生物多样性丰富的北热带喀斯特森林中,木质藤本与支持木的关系鲜为人知。以喀斯特季节性雨林的五桠果叶木姜子（Litsea dilleniifolia）群落为研究对象,对木质藤本的密度、分布格局及其与主要树种的关系进行调查研究,分析木质藤本对树木的影响。结果显示：（1）五桠果叶木姜子群落内木质藤本平均密度为0.0913株/m²,木质藤本在0-20m空间尺度整体表现为聚集分布,且随着尺度增大,聚集强度逐渐减弱;不同径级木质藤本在不同尺度上的分布格局不同。（2）木质藤本对不同径级、不同种类、不同聚集强度的支持木选择表现以下体征：随着支持木径级增加,木质藤本攀附的比例和每木藤本数有增加趋势,且木质藤本胸径与支持木胸径呈极显著正相关;附藤率较高的支持木有紫葳科（Bignoniaceae）种类和东京桐（Deutzianthus tonkinensis）,单木附藤数量多的是南方紫金牛（Ardisia thyrsiflora）;物种的聚集强度与附藤率、附藤数量呈负相关。（3）木质藤本的密度与支持木死亡率关系不显著,而物种的附藤率与死亡率呈极显著负相关。以上结果表明,木质藤本密度在原生性喀斯特季节性雨林中并不高,且木质藤本对支持木具有选择性,但其对五桠果叶木姜子群落的死亡率并未产生显著影响。该研究可为喀斯特原生性季节性雨林的物种共存、极小植物种群保育提供理论依据,也可为石漠化区域的植被修复提供科学参考。     

Insights into functional genes and taxonomical/phylogenetic diversity of microbial communities in biological heap leaching system and their correlation with functions

Applied Microbiology and Biotechnology - Although the taxonomical/phylogenetic diversity of microbial communities in biological heap leaching systems has been investigated, the diversity of...     

XPF with mutations in its conserved nuclease domain is defective in DNA repair but functions in TRF2-mediated telomere shortening

TRF2, a telomere-binding protein, is a crucial player in telomere length maintenance. Overexpression of TRF2 results in telomere shortening in both normal primary fibroblasts and telomerase-positive cancer cells. TRF2 is found to be associated with XPF-ERCC1, a structure-specific endonuclease involved in nucleotide excision repair, crosslink repair and DNA recombination. XPF-ERCC1 is implicated in TRF2-dependent telomere loss in mouse keratinocytes, however, whether XPF-ERCC1 and its nuclease activity are required for TRF2-mediated telomere shortening in human cells is unknown. Here we report that TRF2-induced telomere shortening is abrogated in human cells deficient in XPF, demonstrating that XPF-ERCC1 is required for TRF2-promoted telomere shortening. To further understand the role of XPF in TRF2-dependent telomere shortening, we generated constructs containing either wild type XPF or mutant XPF proteins carrying amino acid substitutions in its conserved nuclease domain. We show that wild type XPF can complement XPF-deficient cells for repair of UV-induced DNA damage whereas the nuclease-inactive XPF proteins fail to do so, indicating that the nuclease activity of XPF is essential for nucleotide excision repair. In contrast, both wild type XPF and nuclease-inactive XPF proteins, when expressed in XPF-deficient cells, are able to rescue TRF2-mediated telomere shortening. Thus, our results suggest that the function of XPF in TRF2-mediated telomere shortening is conserved between mouse and human. Furthermore, our findings reveal an unanticipated nuclease-independent function of XPF in TRF2-mediated telomere shortening.     

Peptidase allergen Der p 1 initiates apoptosis of epithelial cells independently of tight junction proteolysis

Loss of epithelial cell polarity, which can arise following disruption of tight junctions (TJs), is a precursor to the care-fully orchestrated removal of moribund cells from epithelia in apoptosis. Ordinarily, this cycle of events has minimally disruptive effects on the function of the epithelial barrier, but some agents have been identified that induce apoptosis and promote epithelial leakiness. The allergen Der p 1 is a cysteine peptidase that cleaves TJ adhesion proteins and induces apoptosis in epithelial cells. This suggests the possibility that, at least for some inducers of apoptosis, these events might be causally linked. We report here that Der p 1 induces epithelial apoptosis before outright cell detachment and that apoptosis occurs within the same time span as increased paracellular permeability in polarized epithelial monolayers. Whilst TJ-deficient BEAS-2B cells were resistant to Der p 1-induced apoptosis, the cell line 1HAEo-, which was also TJ deficient, was sensitive to Der p 1, providing evidence against TJ proteolysis as a cause of apoptosis. To provide direct evidence, we propagated cells that normally express TJs in low calcium medium that prevented intercellular junction assembly. These cells retained full susceptibility to Der p 1, indicating that Der p 1-induced apoptosis is independent from TJ proteolysis.     

A statistical approach to computer processing of cryo-electron microscope images: virion classification and 3-D reconstruction

The scattering density of the virus is represented as a truncated weighted sum of orthonormal basis functions in spherical coordinates, where the angular dependence of each basis function has icosahedral symmetry. A statistical model of the image formation process is proposed and the maximum likelihood estimation method computed by an expectation-maximization algorithm is used to estimate the weights in the sum and thereby compute a 3-D reconstruction of the virus particle. If multiple types of virus particle are represented in the boxed images then multiple 3-D reconstructions are computed simultaneously without first requiring that the type of particle shown in each boxed image be determined. Examples of the procedure are described for viruses with known structure: (1). 3-D reconstruction of Flockhouse Virus from experimental images, (2). 3-D reconstruction of the capsid of Nudaurelia Omega Capensis Virus from synthetic images, and (3). 3-D reconstruction of both the capsid and the procapsid of Nudaurelia Omega Capensis Virus from a mixture of unclassified synthetic images.