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排序方式: 共有49条查询结果,搜索用时 468 毫秒
1.
Dual wavelength microfluorometry was used to measure the cytoplasmic free calcium concentration [( Ca2+]in) in single cultured cells from ventricular myocytes of neonatal rats loaded with the indicator fura-2. At 2.5 nmol/l extracellular Ca2+ in the resting cells [Ca2+]in was between 80 and 110 nmol/l. Sometimes, spontaneous low-frequency (approximately 0.1 Hz) [Ca2+]in oscillations were observed. High-potassium depolarization led to a Ca2+-antagonists-sensitive rise of [Ca2+]in. Both caffeine++ (5-10 mmol/l) and thymol (lmmol/l) initialized transient increase of [Ca2+]in. Mechanisms of [Ca2+]in homeostasis in heart muscle cells were discussed. 相似文献
2.
P V Belan O N Osipenko A V Tepikin 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1990,96(1):45-47
1. Cytoplasmic Ca2+ concentration ICaIin and membrane potential of single Helix pomatia neurons was studied by Fura-2 fluorescence measurement and conventional current clamp methods. 2. Intracellular injection of inositol-1,4,5-trisphosphate (IP3) and nonhydrolysable GTP analogue (Gpp/NH/p) led to a rise of ICaIin; in contrast, GTP injection did not cause significant ICaIin changes. 3. We suggest that both IP3 and Gpp/NH/p directly activated Ca release from intracellular stores. 相似文献
3.
V D Novikov N T Iasakova O V Lisichenko T V Luk'ianova V S Avdeenko I B Belan 《Tsitologiia》1992,34(7):47-52
Chromatin structure has been studied with a method of optico-structural machine analysis for interphase nuclei of peripheral blood lymphocytes in healthy donors, a girl with the Shereshevski?-Turner syndrome (45, XO) and her parents, and for peripheral blood lymphocytes of a cow, Bos taurus L., in the last trimester on the background of preeclamptic syndrome and with normal cow pregnancy of compared terms. A significant change was revealed both in human heteroploidy and in developing preeclamptic syndrome in animals as concerns such indexes as the nucleus area, integral optical density, the amount and area of condensed, decondensed chromatin. The profile of histograms, according to the given parameters, is distinctly changing. The analysis based on constructing a two-measured field in the given coordinate system showed changes in the lymphatic population structure with the increase in the rate of "heavy", enriched DNA cells. 相似文献
4.
5.
L. I. Laikova I. A. Belan E. D. Badaeva L. P. Rosseeva S. S. Shepelev V. K. Shumny L. A. Pershina 《Russian Journal of Genetics》2013,49(1):89-97
Synthetic hexaploids are bridges for transferring new genes that determine resistance to stress factors from wild-type species to bread wheat. In the present work, the method of developing the spring bread wheat variety Pamyati Maystrenko and the results of its study are described. This variety was obtained using one of the immune lines produced earlier via the hybridization of the spring bread wheat variety Saratovskaya 29 with the synthetic hexaploid T. timopheevii Zhuk. × Ae. tauschii Coss. The C-staining of chromosomes in the Pamyati Maystrenko variety revealed substitutions of 2B and 6B chromosomes by the homeologous chromosomes of the G genome of T. timopheevii and the substitution of chromosome 1D by an orthologous chromosome of Ae. tauschii. It was found that this variety is characterized by resistance to leaf and stem rust, powdery mildew, and loose smut as well as by high grain and bread-making qualities. The role of the alien genetic material introgressed into the bread-wheat genome in the expression of adaptive and economically valuable traits in the Pamyati Maystrenko variety is discussed. 相似文献
6.
T. T. Efremova E. V. Chumanova N. V. Trubacheeva V. S. Arbuzova I. A. Belan L. A. Pershina 《Russian Journal of Genetics》2016,52(2):146-153
With the use of allele-specific primers developed for the VRN1 loci, the allelic diversity of the VRN-A1, VRN-B1, and VRN-D1 genes was studied in 148 spring common wheat cultivars cultivated under the conditions of western Siberia. It was demonstrated that modern Western Siberian cultivars have the VRN-A1a allele, which is widely distributed in the world (alone or in combination with the VRN-B1a and VRN-B1c alleles). It was established that the main contribution in acceleration of the seedling–heading time is determined by a dominant VRN-A1a allele, while the VRNA1b allele, on the contrary, determines later plant heading. Cultivars that have the VRN-A1b allele in the genotype are found with a frequency of 8%. It was shown that cultivars with different allele combinations of two dominant genes (VRN-A1a + VRN-B1c and VRN-A1a + VRN-B1a) are characterized by earlier heading and maturing. 相似文献
7.
N. V. Trubacheeva L. P. Rosseeva I. A. Belan T. S. Osadchaya L. A. Kravtsova Yu. V. Kolmakov N. P. Blokhina L. A. Pershina 《Russian Journal of Genetics》2011,47(1):13-18
Using genomic in situ hybidization, among the common wheat cultivars produced in West Siberia (Siberian Research Institute
of Agriculture, Omsk) with the involvement of the winter wheat cultivar Kavkaz carrying the wheat-rye 1RS.1BL translocation
we identified three cultivars with this translocation: Omskaya 29, Omskaya 37, and Omskaya 38. The protein and crude gluten
contents in the grain of these cultivars are equal to or exceed the levels observed in cultivars without the wheat-rye translocation.
The common wheat cultivars carrying the wheat-rye translocation were evaluated in terms of resistance of plants reaching wax
ripeness to leaf rust and powdery mildew in the natural field conditions. The cultivars Omskaya 37 and Omskaya 38 displayed
a high field resistance to leaf rust and were resistant to a variable extent to powdery mildew. The cultivar Omskaya 29 was
susceptible to leaf rust and powdery mildew pathogens. Importance of the selection direction and the role of the genetic background
in developing common wheat cultivars carrying the wheat-rye translocation is discussed. 相似文献
8.
P. V. Belan E. É. Saftenku O. V. Gerasimenko N. Ch. Pogorelaya M. A. Chvanov V. I. Teslenko 《Neurophysiology》1997,29(1):40-44
We studied spatial and temporal patterns of Ca2+ extrusion from pancreatic acinar cells evoked by acetylcholine(ACh)-induced activation of plasma membrane calcium pumps.
Using a modification of an earlier developed model, we estimated the time course of extracellular calcium concentration changes
near the basal pole of a cell in the case, when calcium ions are released from the same site on the cell surface, and in the
case when they are extruded from the apical pole and diffuse to the basal one. It is concluded that at the first stage of
ACh-induced Ca2+ extrusion the appearance of Ca2+ elevation near the basal pole of the cells cannot be explained as a result of diffusion, but is mainly determined by Ca2+ efflux from this pole. The results also show that there are plasma membrane calcium pumps in both apical and basal parts
of pancreatic acinar cells, but the activity of the pumps is substantially higher in the apical region. 相似文献
9.
A method for quantitative appraisal of disturbances of macrobenthic communities of soft substrata caused by anthropogenic pollution is proposed. This method is based on the establishment of the dependence of various parameters characterizing the abundance and structure of communities (biomass, population density, number of species, Shannon-Weaver and Pielou indices, etc.) on the level of total contamination of bottom sediments. Using the percentage of deviation of abundance parameters and ecological indices from the norm (the initial horizontal segment of model curves), as well as ERL q and ERM q values, which limit the area of progressive degradation of bottom communities (an almost linear drop in abundance and structure characters), is suggested as a numerical characteristic of such deformations. Mapping of aqueous areas on the basis of these characteristics enabled us to obtain a synoptic view of the extent and spatial scale of anthropogenic impacts. An example of the application of this method for assessment of the state of benthic communities in the Peter the Great Bay (Sea of Japan) is shown for the end of the 1980s. The method is verified on the basis of comparison with the results obtained by standard methods of appraisal of the ecological status of macrozoobenthic communities. 相似文献
10.
Miroslav Balaz Marek Vician Zuzana Janakova Timea Kurdiova Martina Surova Richard Imrich Zuzana Majercikova Adela Penesova Miroslav Vlcek Alexander Kiss Vitazoslav Belan Iwar Klimes Juraj Olejnik Daniela Gasperikova Christian Wolfrum Barbara Ukropcova Jozef Ukropec 《Obesity (Silver Spring, Md.)》2014,22(8):1821-1829