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Andr Gomes-dos-Santos Manuel Lopes-Lima Andr M Machado Antnio Marcos Ramos Ana Usi Ivan N Bolotov Ilya V Vikhrev Sophie Breton L Filipe C Castro Rute R da Fonseca Juergen Geist Martin E
sterling Vincent Pri Amílcar Teixeira Han Ming Gan Oleg Simakov Elsa Froufe 《DNA research》2021,28(2)
Since historical times, the inherent human fascination with pearls turned the freshwater pearl mussel Margaritifera margaritifera (Linnaeus, 1758) into a highly valuable cultural and economic resource. Although pearl harvesting in M. margaritifera is nowadays residual, other human threats have aggravated the species conservation status, especially in Europe. This mussel presents a myriad of rare biological features, e.g. high longevity coupled with low senescence and Doubly Uniparental Inheritance of mitochondrial DNA, for which the underlying molecular mechanisms are poorly known. Here, the first draft genome assembly of M. margaritifera was produced using a combination of Illumina Paired-end and Mate-pair approaches. The genome assembly was 2.4 Gb long, possessing 105,185 scaffolds and a scaffold N50 length of 288,726 bp. The ab initio gene prediction allowed the identification of 35,119 protein-coding genes. This genome represents an essential resource for studying this species’ unique biological and evolutionary features and ultimately will help to develop new tools to promote its conservation. 相似文献
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Shtyrlin Valery G. Borissenok Valery A. Serov Nikita Yu. Simakov Vladimir G. Bragunets Vyacheslav A. Trunin Ivan R. Tereshkina Irina A. Koshkin Sergey A. Bukharov Mikhail S. Gilyazetdinov Edward M. Shestakov Evgeny E. Sirotkina Anna G. Zakharov Alexey V. 《Origins of life and evolution of the biosphere》2019,49(1-2):1-18
Origins of Life and Evolution of Biospheres - Syntheses under shock in nitrogen bubbled samples of the water – formamide – bicarbonate – sodium hydroxide system at... 相似文献
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Scribano Vittorio Simakov Sergei K. Finocchiaro Claudio Correale Alessandra Scirè Salvatore 《Origins of life and evolution of the biosphere》2019,49(1-2):19-47
Origins of Life and Evolution of Biospheres - Pyrite and organic matter closely coexist in some hydrothermally-altered gabbroic xenoliths from the Hyblean Plateau, Sicily. The representative sample... 相似文献
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Sportsman-fencers (683 persons) of various qualification and types of fencing (sabre fencers, foil fencers, swordsmen) have been roentgenologically examined. Of them 97 have been examined for the second time in a year. For a comparison 204 persons nonsportsmen have been examined. Increased specialized loadings of chop-push character, peculiar for fencing, accelerate processes of bone sinostosis and result in certain morphological changes in the components of the knee joint: the joint cartilage becomes thinner (as demonstrates the roentgenologically measured joint cleft), periostal osteogenesis on the surface of epiphyses, endothelial osteogenesis in the bone diaphyses become activated. 相似文献
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Expression of secreted Wnt pathway components reveals unexpected complexity of the planarian amputation response 总被引:1,自引:0,他引:1
Kyle A. Gurley Sarah A. Elliott Oleg Simakov Thomas W. Holstein 《Developmental biology》2010,347(1):24-39
Regeneration is widespread throughout the animal kingdom, but our molecular understanding of this process in adult animals remains poorly understood. Wnt/β-catenin signaling plays crucial roles throughout animal life from early development to adulthood. In intact and regenerating planarians, the regulation of Wnt/β-catenin signaling functions to maintain and specify anterior/posterior (A/P) identity. Here, we explore the expression kinetics and RNAi phenotypes for secreted members of the Wnt signaling pathway in the planarian Schmidtea mediterranea. Smed-wnt and sFRP expression during regeneration is surprisingly dynamic and reveals fundamental aspects of planarian biology that have been previously unappreciated. We show that after amputation, a wounding response precedes rapid re-organization of the A/P axis. Furthermore, cells throughout the body plan can mount this response and reassess their new A/P location in the complete absence of stem cells. While initial stages of the amputation response are stem cell independent, tissue remodeling and the integration of a new A/P address with anatomy are stem cell dependent. We also show that WNT5 functions in a reciprocal manner with SLIT to pattern the planarian mediolateral axis, while WNT11-2 patterns the posterior midline. Moreover, we perform an extensive phylogenetic analysis on the Smed-wnt genes using a method that combines and integrates both sequence and structural alignments, enabling us to place all nine genes into Wnt subfamilies for the first time. 相似文献
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To understand the initial stages of membrane destabilization induced by viral proteins, the factors important for binding of fusion peptides to cell membranes must be identified. In this study, effects of lipid composition on the mode of peptides' binding to membranes are explored via molecular dynamics (MD) simulations of the peptide E5, a water-soluble analogue of influenza hemagglutinin fusion peptide, in two full-atom hydrated lipid bilayers composed of dimyristoyl- and dipalmitoylphosphatidylcholine (DMPC and DPPC, respectively). The results show that, although the peptide has a common folding motif in both systems, it possesses different modes of binding. The peptide inserts obliquely into the DMPC membrane mainly with its N-terminal alpha helix, while in DPPC, the helix lies on the lipid/water interface, almost parallel to the membrane surface. The peptide seriously affects structural and dynamical parameters of surrounding lipids. Thus, it induces local thinning of both bilayers and disordering of acyl chains of lipids in close proximity to the binding site. The "membrane response" significantly depends upon lipid composition: distortions of DMPC bilayer are more pronounced than those in DPPC. Implications of the observed effects to molecular events on initial stages of membrane destabilization induced by fusion peptides are discussed. 相似文献
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The amino acid sequences of some fiber proteins possibly have a periodic structure. This periodicity can be analyzed using the Fourier transform of the mathematical image of the symbol sequence of amino acid residues in proteins. One of several possible methods of Fourier transform has been chosen as optimal for the given study. This optimal Fourier transform has been used to analyze the periodic structures in several fiber proteins of bacteriophage T4. Amino acids from some groups form sequences of alternating elements with a relatively small period (T=15); those from other groups form sequences with other small periods (T=10 and T=8). Relatively large periods of amino acid arrangement, with the entire amino acid sequence of the protein being divided between them into four or six equal parts, is a new finding. The data on protein structural periodicity make it possible to align the amino acid sequences according to the periodic structures of both type. The results obtained agree with the results of previous crystallographic and electron microscopic studies.__________Translated from Molekulyarnaya Biologiya, Vol. 39, No. 2, 2005, pp. 321–329.Original Russian Text Copyright © 2005 by Simakova, Simakov. 相似文献