steve bAccumulation of nerve growth factor and its receptors in the uterus and dorsal root ganglia in a mouse model of adenomyosis

Background  

Adenomyosis is a common gynecological disease, which is accompanied by a series of immunological and neuroendocrinological changes. Nerve growth factor (NGF) plays a critical role in producing pain, neural plasticity, immunocyte aggregation and release of inflammatory factors. This study aimed to investigate the expression of NGF and its two receptors in uteri and dorsal root ganglia (DRG) in an adenomyosis mouse model, as well as their relationship with the severity of adenomyosis.     

口服他莫昔芬法建立ICR小鼠子宫腺肌病模型

目的使用口服他莫昔芬法建立ICR小鼠子宫腺肌病模型,并检测其病灶特征、动情周期、血管生成、子宫炎症等变化,以介绍和评价这一动物模型。方法新生ICR小鼠（15只）连续4 d滴喂他莫昔芬,并与同龄对照小鼠（15只）分别于42、85-95、135-145日龄处死,使用苏木素-伊红染色检测子宫病理改变;阴道脱落细胞法检测动情周期变化;免疫组化补体31（CD31）染色计算子宫微血管的密度、直径及所占面积比;逆转录-聚合酶链反应（RT-PCR）检测子宫缓激肽受体、神经激肽受体的基因表达。结果使用口服他莫昔芬法建立ICR小鼠子宫腺肌病模型的造模率为100%,且疾病严重程度随病程进展。部分给药小鼠可出现动情周期紊乱。85-95及135-145日龄给药小鼠子宫肌层微血管密度和面积比均高于对照小鼠（P〈0.05）。135-145日龄给药小鼠子宫缓激肽受体、神经激肽受体的基因表达较对照组明显升高（P〈0.05）。结论口服他莫昔芬法可方便、高效的建立腺肌病小鼠模型,出现腺肌病相关的血管生成、炎症状态、疼痛相关受体表达增高等特征,是研究腺肌病发生、发展的良好模型。     

滤减UV-B辐射对烟叶腺毛发育和密度动态变化的影响

以烟草品种"K326"为材料,盆栽种植于自然环境条件下(CK)和覆盖不同厚度透明薄膜,分别滤减25%(T1)、50%(T2)和65%(T3)UV-B辐射的大棚中,在"K326"开花期至工艺成熟后期的4个生育期,通过扫描电镜观察了各处理烟叶腺毛形态和密度动态变化特征,并首次报道了"K326"中存在分枝腺毛。结果表明:上表皮腺毛在开花期、生理成熟期和工艺成熟前期对UV-B辐射敏感,下表皮则在开花期和工艺成熟后期对UV-B辐射敏感;T3处理腺毛密度低且延缓腺毛的发育,T1和T2处理腺毛密度较高,在工艺成熟期出现二次发育现象;各处理烟叶腺毛以长柄腺毛为主,CK、T1、T2下表皮腺毛总密度大于上表皮,而T3上、下表皮差异不大;滤减UV-B辐射有利于烟叶下表皮长柄腺毛发育,而引起腺毛二次发育的腺毛类型依处理和时期而异;适当较高强度的UV-B辐射对烟叶腺毛发育有促进作用,在研究地减弱25%～50%的UV-B辐射强度对烟叶腺毛发育较为合适。     

如何在微生物检验专题实验教学中体现应用型人才培养

在食品检验、化妆品检验和环境水检测等微生物检验专题实验中采取了一些改进措施。实践证明,这些措施有助于提高学生的学习能动性,增强其动手动脑能力。     

烟草形态和光合生理对减弱UV-B辐射的响应

在云南较高海拔烟区,通过大棚覆膜减弱UV-B辐射,研究了烟草品种K326生理成熟期、工艺成熟期和生理成熟向工艺成熟的过渡期形态和光合生理对减弱UV-B辐射(T₁ 75.74%、T₂ 70.08%、T₃ 30.39%)的响应.结果表明： 减弱UV-B辐射显著增加了K326的茎高和节间距,在T₂下茎高和节间距较大;与自然环境处理（CK）相比,T₁和T₂降低了K326的净光合速率、同化能力、水分利用率、内在水分利用率、光合色素和类黄酮含量及比叶重等,但T₁各指标大于T_2.影响T₁和T₂净光合速率的因素有气孔因素和非气孔因素,以非气孔因素为主,气孔调节能力较低导致的蒸腾速率增大是造成两处理水分利用率较低的主要原因.T₃处理在生理成熟期和过渡期对净光合速率、同化能力、水分利用率、内在水分利用率和光合色素均有一定的促进作用,而比叶重和类黄酮则处于最低水平,工艺成熟期的光合色素降解也较其他处理快.     

用硫酸亚铁溶液吸收处理氮氧化物的效果

实验分析探讨了FeSO4溶液对氮氧化物(NOx)的吸收去除机理,并考察了Fe2+初始质量分数、混合气中O2体积分数、NOx初始质量浓度和气液接触时间对NOx去除率的影响.结果表明,FeSO4吸收液在吸收初期对NOx表现出了较好的吸收去除效果.Fe2+初始质量分数越高、O2体积分数越大、NOx初始质量浓度越低、气液接触时间越长,NOx的去除率就越高.在吸收剂中Fe2+质量分数为4%、模拟废气中O2体积分数为34.9%的条件下,FeSO4吸收液对NOx初始质量浓度为2700 mg·m-3的废气进行吸收去除时,在吸收反应初期阶段,NOx的去除率可以达到80%以上.     

steve bAccumulation of nerve growth factor and its receptors in the uterus and dorsal root ganglia in a mouse model of adenomyosis

Background

The pregnancy hormone human chorionic gonadotropin (hCG) and its free subunits (hCG alpha, hCG beta) are produced in the male reproductive tract and found in high concentrations in seminal fluid, in particular hCG alpha. This study aimed to elucidate changes in peptide hormone profiles in patients showing abnormal semen analyses and to determine the genuineness of the highly abundant hCG alpha.

Methods

Seminal plasma was obtained from 45 male patients undergoing semen analysis during infertility workups. Comprehensive peptide hormone profiles were established by a panel of immunofluorometric assays for hCG, hCG alpha, hCG beta and its metabolite hCG beta core fragment, placental lactogen, growth hormone and prolactin in seminal plasma of patients with abnormal semen analysis results (n = 29) versus normozoospermic men (n = 16). The molecular identity of large hyperglycosylated hCG alpha was analyzed by mass-spectrometry and selective deglycosylation.

Results

hCG alpha levels were found to be significantly lower in men with impaired semen quality (1346 +/- 191 vs. 2753 +/- 533 ng/ml, P = 0.022). Moreover, patients with reduced sperm count had reduced intact hCG levels compared with normozoospermic men (0.097 +/- 0.022 vs. 0.203 +/- 0.040 ng/ml, P = 0.028). Using mass-spectrometry, the biochemical identity of hCG alpha purified from seminal plasma was verified. Under non-reducing conditions in SDS-PAGE, hCG alpha isolated from seminal plasma migrated in a manner comparable with large free hCG alpha with an apparent molecular mass (Mr, app) of 24 kDa, while hCG alpha dissociated from pregnancy-derived holo-hCG migrated at approximately 22 kDa. After deglycosylation with PNGase F under denaturing conditions, all hCG alpha variants showed an Mr, app of 15 kDa, indicating identical amino acid backbones.

Conclusions

The findings indicate a pathophysiological relevance of hCG, particularly its free alpha subunit, in spermatogenesis. The alternative glycosylation pattern on the free large hCG alpha in seminal plasma might reflect a modified function of this subunit in the male reproductive tract.     

白术多糖WAM-1结构的色谱分析和原子力显微镜观察

通过热水浸提法从草本植物白术根茎提取的水溶性粗多糖,经DEAE-52纤维素柱层析分离和Sephadex G-200凝胶过滤柱层析纯化,得到组分WAM-1.采用高效液相色谱(HPLC)检测WAM-1的纯度,气相色谱(GC)对其单糖组分进行分析,原子力显微镜(AFM)对其分子外貌进行观测.结果显示:WAM-1为均一多糖,由葡萄糖和半乳糖以3.01:1摩尔比构成;在不同浓度溶液条件下,WAM-1分子以不同形态存在,多糖溶液的浓度对WAM-1的分子链构象及链间相互作用形式产生影响,推测可能与WAM-1分子内、分子间的氢键缔合作用有关.多糖浓度为10μg/mL时,可清晰的观察到WAM-1是以刚性链状形态存在,且具有多分支结构.     

低纬高原不同利用方式土壤对烟草生长及光合生理的影响

以取自低纬高原云南玉溪烟区不同地域稻田(峨山,E)、麦田(北城1,B1;北城2,B2)、山坡旱地(新平,X)和菜地(通海四街,T1;通海桑园,T2)的6种不同利用方式土壤盆栽种植烟草品种K326,研究了在相同气候背景和水肥管理条件下,不同土壤对K326生长和光合生理的影响。结果表明:K326在山坡旱地土、稻田土和麦田土上生长较菜地土上好(北城1除外),且新平烟叶净光合速率(Pn)较高,新平和峨山烟叶气孔导度(Gs)、胞间CO2浓度(Ci)和蒸腾速率(Tr)较大,而气孔限制值(Ls)、水分利用效率(WUE)和内在水分利用效率(IWUE)较小。分析表明,麦田和菜地土壤上生长的K326其Pn主要受气孔因素的影响,而稻田土壤上K326的Pn主要受羧化能力低、光合色素和可溶性蛋白含量低等非气孔因素的影响。峨山和新平K326的气孔调节能力较差,其较高的土壤含水量使Gs增大,Tr上升,从而导致WUE下降。各土壤处理烟叶的鲜重含水量和自然水分饱和亏没有显著差异,菜地土烟叶单位面积含水量显著低于稻田土,与其比叶重(SLM)有一定联系。结果说明了土壤对烟草的影响较为复杂,既与土壤本身的特性有关,又与不同土壤条件下植物本身的生理特性有关。