Production of Potato Spindle Tuber Viroid (PSTV) by Large Scale Fermentation of PSTY-Infected Potato Cell Suspension Cultures

Berlin, J., Wray, V., Forche, E., Reng, H.–G , Schler,H, Luckinger, R. and Mhlbach, H.–P. 1985. Production ofpotato spindle tuber viroid (PSTV) by large scale fermentationof PSTV–infected potato cell suspension cultures.—J.exp. Bot 36: 1985–1995. Cell suspension cultures of Solatiumdemissum, infected with the potato spindle tuber viroid (PSTV),were scaled up to volumes of up to 800 dm³ to provide sufficientand uniform plant material for subsequent studies on viroidbiosynthesis. Here we describe the technological aspects ofproducing the required amounts of biomass and viroid. The cells,which had been maintained on a medium containing expensive coconutmilk, were first adapted to rapid growth on the less expensiveB5–medium. The physiological state of the cells was monitoredby in vivo ³¹P–NMR spectroscopy Under the chosen conditionsthe scale–up from 10 dm³ inoculum from shake flasks tothe harvest of the 800 dm³ stirred fermenter lasted 38 d andprovided 112 kg biomass. Growth characteristics and viroid productionin shake flasks and large bioreactors were rather similar. Gelelectrophoretic analysis of isolated nucleic acids using silverstaining and Northern blot hybridization revealed a PSTV–contentof approximately 700 µg PSTV per kg fresh mass of culturedcells. Key words: Solanum demissum, plant cell cultures, potato spindle tuber viroid, biomass production, fermentation, in vivo ³¹P-NMR     

茶叶提取物在癌症临床防治中的生理效应

根据大量的临床研究资料对茶叶提取物在癌症防治中的生理效应进行了论述 .茶叶提取物可以改善血流变、微循环状态 ,在放疗、化疗中保护正常细胞 ,增强机体免疫功能 ,降低血清唾液酸浓度 ,改善临床症状 ,提高生命质量 ,延长患者生命     

Species Diversity and Floristic Characteristics of Vascular Plants in Nanji Island,Zhejiang Provience

A total of 393 species (sub type included) in 267 genera, 97 families were reported in Nanji Island, the main island of Nanji Islands National Nature Reserve. After eliminating all the cultivated and invasive species, there were still 304 species in 210 genera and 81 families belonging to wild vascular plants; To better understand the floristic characteristics of Nanji, we applied analysis in 3 levels: At family level, the largest families in pteridophyte were ranked as Pteridaceae、Thelypteridaceae、Lygodiaceae、Dryopteridaceae; In seed plants, almost the largest ones were Cosmopolitan, while the small ones dominented the most, quantitatively and proportionately; The characteristic families of seed flora were consisted of Liliaceae、Amaryllidaceae、Urticaceae、Verbenaceae, cause they got high ratio of VFICS/WFIW; At genus level, the Pantropical Distribution (3810%)、the North Temperate Distribution (1630%) and the East Asia distribution (1131%) formed the main part of seed flora; The proportion of the category of temperate distribution and tropical distribution was 6072% and 3928% respectively; Took 6 other sites as reference, through cluster analysis and R/T ratio comparisons, It was proved that Nanji Island were most similar to that of Wuyanling of Zhejiang Provience but showed more tropical affinities; At species level, wild seed plants can also be divided into 9 areal subtypes, The Yangtze River & Southern Proviences sub type dominented at 5390%, following by South China sub type at 2078%. The results showed that Nanji Island had a distinctive transitional characteristics of flora from north to south China with more tropical biological properties, thus the floristic division of Nanji should on the southern margin area of east China.     

辛酸沉淀结合阴离子交换层析纯化马IgG的研究

目的以辛酸沉淀结合离子交换层析纯化破伤风抗毒素马免疫血浆,获得高质量的马IgG,为抗毒素F(ab')2的制备奠定基础。方法通过对辛酸沉淀马血浆过程中的pH、辛酸浓度以及血浆稀释倍数的实验设计(DoE),研究不同条件对IgG纯度、效价、比活性、浊度以及过滤速度的影响,确定各工艺参数的可操作区间,并结合Capto DEAE阴离子交换层析以流穿模式进一步纯化IgG。结果马血浆经一步辛酸沉淀获得的IgG纯度(SDSPAGE)大于92%、分子排阻色谱(SEC)纯度大于95%,比活性较血浆提高2.14±0.29倍;辛酸沉淀后的IgG样品经阴离子交换层析,可有效去除聚合体以及小分子杂质,将纯度提高至95%(SDS-PAGE)和98%(SEC)。结论马血浆经辛酸沉淀和阴离子交换层析可获得高纯度、高比活的IgG。     

狂犬病病毒CTN-1V株在人二倍体细胞Walvax-2株的适应传代

目的建立狂犬病病毒固定毒CTN-1V株在人二倍体细胞Walvax-2株上的传代适应株。方法用狂犬病病毒固定毒CTN-1V株经昆明小鼠鼠脑回传后的病毒接种Walvax-2细胞,连续传代,检测各代次病毒的滴度及免疫原性。结果 CTN-1V株能较好地适应Walvax-2细胞,通过连续带毒传代至第7代,病毒滴度可达6.78 lg LD50/mL,并在第10~15代内滴度维持在7.0 lg LD50/mL以上,15~30代滴度稳定在7.0 lg LD50/mL左右。以15代适应毒株CTN-1V-HDC P15制备的疫苗原液,各项指标均符合《中华人民共和国药典》三部(2010版)的要求,疫苗效力在6.0IU/剂以上。结论所获人胚肺二倍体细胞Walvax-2株传代适应狂犬病毒固定毒株CTN-1V-HDC可用于人用狂犬病疫苗的生产开发。