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Alkalinity of the medium was shown to be the chief factor involved in the accumulation of oxalate by T. cinnabarina. Glutamate and aspartate are shown to lead to oxalate with this organism and with L. lepideus by dehydrogenation to α-ketoglutarate and oxaloacetate, respectively. Malate was also shown to be dehydrogenated. It is proposed that oxaloacetate may either undergo β-decarboxylation to yield CO2 and pyruvate, or splitting by coenzyme A to yield oxalate and acetylated coenzyme A. The reversal of this latter reaction is suggested as the explanation of the disappearance of oxalate from culture media. The reduction of resazurin by the dehydrogenase systems of the molds is inhibited by cyanide, indicating the participation of metal systems, such as the cytochromes. 相似文献
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Quini CC Américo MF Corá LA Calabresi MF Alvarez M Oliveira RB Miranda JR 《Journal of biological engineering》2012,6(1):6-6
ABSTRACT: AC Biosusceptometry (ACB) was previously employed towards recording gastrointestinal motility. Our data show a reliable and successful evaluation of gastrointestinal transit of liquid and solid meals in rats, considering the methods scarcity and number of experiments needed to endorsement of drugs and medicinal plants. ACB permits real time and simultaneous experiments using the same animal, preserving the physiological conditions employing both meals with simplicity and accuracy. 相似文献
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A new method for the determination of the lignin in cork has been reported. This procedure gives values ca. 25–30%. It is based on the colorimetric estimation of the lignin released by autoclaving with alkali. Using this method it was shown that cork is highly resistant to decay by brown-rot wood-destroying molds. This is attributed to the protective action of the suberin or to the presence in the cork of substances toxic to the molds. 相似文献
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Several methods were employed for the preparation of salt-free trypsin samples which were used to determine the electrometric titration curves of the enzyme. These curves point to a maximum acid-binding capacity below pH 2. Stoichiometric analysis indicates the presence of 6 carboxyl groups per 10,000 g. of proteins, 1 imidazole group, and 13 hydroxyl-binding groups. Calcium has a specific effect on the titration curves by increasing the acidity of the carboxyl groups in the pH range 3.5 – 5. This effect is not shown by potassium, sodium, or even the bivalent magnesium ion. It is attributed to the formation of a specific complex between the enzyme and the calcium ions, involving the carboxyl groups of the protein. The equally specific protective effect of calcium on the self-digestion of trypsin can therefore be explained by assuming the formation of a complex which stabilizes the enzyme. 相似文献
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