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排序方式: 共有116条查询结果,搜索用时 125 毫秒
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CLARE J. HOPKINS NOEL O. I. COGAN MELANIE HAND ERICA JEWELL JATINDER KAUR XI LI GERALDINE A. C. LIM ALISON E. LING CHRISTOPHER LOVE HAYLEY MOUNTFORD MARIJA TODOROVIC MEGAN VARDY GERMAN C. SPANGENBERG DAVID EDWARDS JACQUELINE BATLEY 《Molecular ecology resources》2007,7(4):697-700
The availability of expressed sequence data derived from gene discovery programs enables mining for simple sequence repeats (SSR), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development and characterization of 16 expressed sequence tags (EST)‐SSR markers from Brassica juncea and their cross‐amplification across Brassica species. Sixteen primer pairs were assessed for polymorphism in all genomes of the diploid and amphidiploid Brassica species. The markers show reliable amplification, considerable polymorphism and high transferability across species, demonstrating the utility of EST‐SSRs for genetic analysis of brassicas. 相似文献
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JACQUELINE BATLEY CLARE J. HOPKINS NOEL O. I. COGAN MELANIE HAND ERICA JEWELL JATINDER KAUR SUKHJIWAN KAUR XI LI ALISON E. LING CHRISTOPHER LOVE HAYLEY MOUNTFORD MARIJA TODOROVIC MEGAN VARDY MARZENA WALKIEWICZ GERMAN C. SPANGENBERG DAVID EDWARDS 《Molecular ecology resources》2007,7(5):886-889
The availability of expressed sequence data derived from gene discovery programs enables mining for simple sequence repeats (SSR), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study presents the development and characterization of 24 expressed sequence tags (EST)‐SSR markers from Brassica napus and their cross‐amplification across Brassica species. The markers show reliable amplification, genome specificity and considerable polymorphism, demonstrating the utility of EST‐SSRs for genetic analysis of wild Brassica populations and commercial Brassica germplasm. 相似文献
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SUMMARY. 1. Five populations of the cladoceran Simocephalus vetulus (O. F. Muller) examined by enzyme electrophoresis at four to five loci revealed high levels of genetic variation.
2. The one intermittent population had genotype frequencies in Hardy Weinberg equilibrium, the three permanent populations showed deviations from equilibrium of varying magnitude. The fifth population was fixed for a single clone, and was possibly recently derived from a single ephippial hatchling.
3. Laboratory breeding experiments confirmed that this species is a cyclic parthenogen. 相似文献
2. The one intermittent population had genotype frequencies in Hardy Weinberg equilibrium, the three permanent populations showed deviations from equilibrium of varying magnitude. The fifth population was fixed for a single clone, and was possibly recently derived from a single ephippial hatchling.
3. Laboratory breeding experiments confirmed that this species is a cyclic parthenogen. 相似文献
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