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1.
Marchant J Cowper B Liu Y Lai L Pinzan C Marq JB Friedrich N Sawmynaden K Liew L Chai W Childs RA Saouros S Simpson P Roque Barreira MC Feizi T Soldati-Favre D Matthews S 《The Journal of biological chemistry》2012,287(20):16720-16733
Toxosplasma gondii is the model parasite of the phylum Apicomplexa, which contains numerous obligate intracellular parasites of medical and veterinary importance, including Eimeria, Sarcocystis, Cryptosporidium, Cyclospora, and Plasmodium species. Members of this phylum actively enter host cells by a multistep process with the help of microneme protein (MIC) complexes that play important roles in motility, host cell attachment, moving junction formation, and invasion. T. gondii (Tg)MIC1-4-6 complex is the most extensively investigated microneme complex, which contributes to host cell recognition and attachment via the action of TgMIC1, a sialic acid-binding adhesin. Here, we report the structure of TgMIC4 and reveal its carbohydrate-binding specificity to a variety of galactose-containing carbohydrate ligands. The lectin is composed of six apple domains in which the fifth domain displays a potent galactose-binding activity, and which is cleaved from the complex during parasite invasion. We propose that galactose recognition by TgMIC4 may compromise host protection from galectin-mediated activation of the host immune system. 相似文献
2.
Savvas Saouros Kovilen Sawmynaden Jan Marchant Peter Simpson Stephen Matthews 《Biomolecular NMR assignments》2008,2(2):175-177
Microneme protein complexes are important for invasion of host cells by Toxoplasma gondii. We report the resonance assignment of the galectin-like domain of microneme protein 1 in complexes with the second and third
EGF domains from microneme protein 6.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
3.
Kovilen Sawmynaden Savvas Saouros Jan Marchant Peter Simpson Stephen Matthews 《Biomolecular NMR assignments》2008,2(2):187-189
Toxoplasma gondii is the causative agent of toxoplasmosis. Here we present a complete set of NMR assignments for the second EGF domain from
microneme protein 6 and its re-assignment in complex with the galectin-like domain from microneme protein 1. 相似文献
4.
Jan Marchant Kovilen Sawmynaden Savvas Saouros Peter Simpson Stephen Matthews 《Biomolecular NMR assignments》2008,2(2):119-121
Microneme protein 4 is involved in cell binding by the important parasite Toxoplasma gondii. We present here the backbone and side-chain assignments of the first two apple domains together with a new graphical aid
for their assignment using NMRView.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
5.
Rhiannon Parkhouse Joseph P. Boyle Sophie Mayle Kovilen Sawmynaden Katrin Rittinger Tom P. Monie 《FEBS letters》2014
NOD2 activation by muramyl dipeptide causes a proinflammatory immune response in which the adaptor protein CARD9 works synergistically with NOD2 to drive p38 and c-Jun N-terminal kinase (JNK) signalling. To date the nature of the interaction between NOD2 and CARD9 remains undetermined. Here we show that this interaction is not mediated by the CARDs of NOD2 and CARD9 as previously suggested, but that NOD2 possesses two interaction sites for CARD9; one in the CARD–NACHT linker and one in the NACHT itself. 相似文献
6.
High-level bacterial expression and purification of apicomplexan micronemal proteins for structural studies 总被引:4,自引:0,他引:4
Saouros S Blumenschein TM Sawmynaden K Marchant J Koutroukides T Liu B Simpson P Carpenter EP Matthews SJ 《Protein and peptide letters》2007,14(5):411-415
The cysteine-rich N-terminal domain of the micronemal adhesive protein MIC1 (MIC1-NT) from Toxoplasma gondii was cloned, expressed in Escherichia coli and purified. MIC1-NT is amenable to structural studies as shown by preliminary NMR and X-ray analysis. Positive results with two further micronemal proteins indicate that our strategy has wider application. 相似文献
7.
Jan Marchant Ben Cowper Yan Liu Livia Lai Camila Pinzan Jean Baptiste Marq Nikolas Friedrich Kovilen Sawmynaden Lloyd Liew Wengang Chai Robert A. Childs Savvas Saouros Peter Simpson Maria Cristina Roque Barreira Ten Feizi Dominique Soldati-Favre Stephen Matthews 《The Journal of biological chemistry》2012,287(37):31455
8.
We tested here whether glucocorticoids modulated myeloid cell expression of a specific G-coupled receptor, termed formyl-peptide receptor like-1 (FPRL-1), recently shown to mediate the anti-inflammatory actions of annexin-A1. Real-time PCR and flow cytometry demonstrated rapid up-regulation of mRNA followed by the protein in HL-60 cells incubated with dexamethasone, with peaks at 2 and 24h, respectively. This effect was not restricted to dexamethasone, since reproduced by glucocorticoids. In addition, it was not restricted to the cell line, since replicated with human peripheral blood monocytes. Glucocorticoid ability to upregulate cell surface expression of FPRL-1 was specific, since no effects upon the related receptor FPR or the integrin CD11b could be detected. In view of the wide range of endogenous ligands known to interact with FPRL-1, including the anti-inflammatory protein annexin-A1, we speculate that the novel effect here described may impact on the clinical immunosuppressive and anti-inflammatory properties of glucocorticoids. 相似文献
9.
Gavins FN Sawmynaden P Chatterjee BE Perretti M 《Prostaglandins, leukotrienes, and essential fatty acids》2005,73(3-4):211-219
The inflammatory response is a life-saving protective process mounted by the body to overcome pathogen infection and injury; however, in chronic inflammatory pathologies this response can become deregulated. The existence of specialized anti-inflammatory pathways/mediators that operate in the body to down-regulate inflammation have now emerged. Thus, persistence of inflammation leading to pathology could be due to malfunctioning of one or more of these counter-regulatory pathways. Here we focus on one of them, the anti-inflammatory mediator annexin 1, and provide an update on its inhibitory effects upon the leukocyte trafficking process. In particular, recent evidence that receptors of the formyl-peptide family, which includes also the lipoxin A4 receptor, could be the annexin 1 receptor(s) in the context of anti-inflammation might provide new avenues for exploiting this pathway for drug discovery. 相似文献
10.
Bing Liu Kovilen Sawmynaden Jan Marchant Peter Simpson Stephen Matthews 《Biomolecular NMR assignments》2009,3(1):81-83
Toxoplasma gondii is an obligate parasite that infects most warm blood animals. Micronemal proteins actively involves in the invasion process,
where TgMIC2 and TgM2AP complex plays vital roles. Complete NMR assignments for major fragment of TgM2AP were successfully
obtained. 相似文献