全文获取类型
收费全文 | 742篇 |
免费 | 52篇 |
出版年
2021年 | 4篇 |
2019年 | 4篇 |
2018年 | 10篇 |
2017年 | 7篇 |
2016年 | 9篇 |
2015年 | 10篇 |
2014年 | 12篇 |
2013年 | 36篇 |
2012年 | 39篇 |
2011年 | 51篇 |
2010年 | 12篇 |
2009年 | 18篇 |
2008年 | 69篇 |
2007年 | 48篇 |
2006年 | 49篇 |
2005年 | 60篇 |
2004年 | 62篇 |
2003年 | 36篇 |
2002年 | 46篇 |
2001年 | 13篇 |
2000年 | 12篇 |
1999年 | 19篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 5篇 |
1995年 | 14篇 |
1994年 | 8篇 |
1993年 | 8篇 |
1992年 | 5篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 7篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 6篇 |
1984年 | 7篇 |
1982年 | 4篇 |
1981年 | 5篇 |
1980年 | 6篇 |
1979年 | 2篇 |
1978年 | 5篇 |
1977年 | 4篇 |
1976年 | 7篇 |
1975年 | 3篇 |
1974年 | 6篇 |
1972年 | 2篇 |
1967年 | 5篇 |
1966年 | 2篇 |
1964年 | 2篇 |
排序方式: 共有794条查询结果,搜索用时 62 毫秒
1.
T Hoshino Y Mikura H Shimidzu S Kusumoto J Kawai H Toguchi 《Biochimica et biophysica acta》1987,916(2):245-250
The fluorescence intensity of a unique tryptophan 36 in human interferon-gamma was drastically decreased below pH 4 with a concomitant decrease of antiviral activity. The region of residues 32-42 of human interferon-gamma was found by calculation to have a low hydrophobicity together with a high helical hydrophobic moment, and the net electric charge of this region having an amphiphilic helical structure changed significantly near pH 4. These results suggest that the region of residues 32-42 plays an important role in exhibiting antiviral activity. 相似文献
2.
Gene structure of human thrombomodulin, a cofactor for thrombin-catalyzed activation of protein C 总被引:2,自引:0,他引:2
T Shirai S Shiojiri H Ito S Yamamoto H Kusumoto Y Deyashiki I Maruyama K Suzuki 《Journal of biochemistry》1988,103(2):281-285
The gene coding for human thrombomodulin, a thrombin receptor on endothelial cells and a cofactor for the activation of anticoagulant protein C zymogen, was isolated from a human genomic library by employing human thrombomodulin cDNA as a probe. The nucleotide sequences of the gene and the adjacent 5' and 3' flanking regions were then determined. The nucleotide sequence of this gene with approximately 3.7 kilobase pairs was identical to that of the cDNA, indicating that the gene for human thrombomodulin is free of introns. Hybridization data showed that there is only a single thrombomodulin gene in the human genome. 相似文献
3.
Prof. Katsuko Kataoka Junko Miura Yasuko Takeoka Yoshisuke Kusumoto Noboru Yanaihara 《Cell and tissue research》1985,239(3):531-535
Summary Ontogenesis of gastrin cells was studied in the pyloroduodenal mucosa of the mouse using anti-human G17 serum, R-1301, and anti-human G34(1–15) serum, R-2703. R-1301-immunostained cells first appeared in the pyloric mucosa of 14-day-old fetuses. Cells stained with both R-1301 and R-2703 appeared immediately after birth, and gradually increased in number to the adult level. Most R-1301-reactive cells were also reactive to R-2703, whereas some cells that reacted with R-1301 exhibited very weak or no reaction with R-2703. The discrepancy between these two immunoreactivities is discussed.In the duodenum, a considerable number of R-1301-reactive cells were present from the perinatal stage and through out adult development. A few R-2703-reactive cells were seen in the duodenum of young mice but not of the adult. 相似文献
4.
5.
6.
Mariyo F. Watanabe Ken-Ichi Harada Kennji Matsuura Masayuki Watanabe Makoto Suzuki 《Journal of applied phycology》1989,1(2):161-165
Changes in the content of cyclic heptapeptide hepatotoxins called microcystins were investigated during batch culture of two
Microcystis species using high performance liquid chromatography. After adsorption to ODS-silica gel cartridges and elution with methanol,
the toxins were analyzed and quantified by HPLC. 35 μg per 100 mg dry cells of microcystin-RR, 34 μg of -YR and 43 μg of -LR
were present at the beginning of the exponential growth phase of M. viridis. Microcystin-RR increased markedly towards the end of the exponential phase with the maximum content of 112 μg per 100 mg
cells was measured at the late stage of the exponential phase. A remarkable increase of microcystin-YR from 130 μg per 100
mg cells to 1020 μg was observed during the exponential phase of a highly toxic strain of M. aeruginosa. However no clear differences were found in the pattern of change among the three toxins during the growth course. 相似文献
7.
Endotoxic properties of chemically synthesized lipid A analogs. Studies on six inflammatory reactions in vivo, and one reaction in vitro 总被引:1,自引:0,他引:1
M Yoshida M Hirata K Inada N Tsunoda T Kirikae T Onodera Y Ishikawa O Sasaki T Shiba S Kusumoto 《Microbiology and immunology》1989,33(10):797-810
Biological activities of two groups of synthesized lipid A analogs, the counterpart of biosynthetic precursor, Lehmann's Ia type, 406, and E. coli lipid A type, 506, as well as their non-phosphorylated, and mono-phosphorylated analogs were investigated. The activities employed included four bone marrow cell reactions in mice, mice skin reaction, leukocytes migration in rabbits' cornea, and hemagglutination. Compound 406 and 506 elicited bone marrow reactions in mice and hemagglutination of mouse RBC, although 406 failed to elicit hemorrhage and necrosis also in mice skin. Compound 406 did not elicit corneal reaction in rabbits. The results suggest that for elicitation of this reaction and mice skin reaction, acyloxyacyl structure is required. Cytotoxicity and thromboplastin production of four bone marrow reactions had been reported by us to be endotoxic reactions, since these had not been elicited by peptidoglycan of Lactobacillus and Staphylococcus (1981) and 300 series synthesized analogs (1984) which did not have endotoxic structures. From these results, it seems that these two marrow reactions and hemagglutination require, as does the limulus test, the lipid A part structure as is present in 406. 相似文献
8.
9.
Chieko Takaya Ayako Kosaka Kyouko Kohno Toshihisa Kusano Ken-Ichi Nakamura 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1995,112(4):727-732
Tetrahymena thermophila could still swim after incubation of the cell body at 40°C for 30 min, whereas Tetrahymena pyriformis did not show any motility after the treatment. Turbidity measurements revealed that axonemes of T. pyriformis lost ATP-dependent sliding activity by the heat treatment, whereas those of T. thermophilia still had the activity under the same conditions. In connection with this difference in susceptibility to high temperature, the biochemical characteristics of dyneins were compared between the two species of Tetrahymena. Axonemal dyneins from the two species had significant vanadate-sensitive ATPase activity even after the heat treatment. Native gel electrophoresis and the following two-dimensional electrophoresis showed that the outer arm dynein of T. thermophilia is more stable in maintaining native configuration than that of T. pyriformis against the heat treatment, although both treated dyneins keep three (α, β and γ) subunits. Analysis by peptide mapping demonstrated that β- and γ-subunits of the outer arm dynein are considerably different in amino acid sequences between the two species. These results imply that dynein of T. thermophilia changed their amino acid sequences and biochemical characteristics to adapt to high temperature. 相似文献
10.
Ken-Ichi Iwasaki Rong Zhang Julie H Zuckerman Benjamin D Levine 《Journal of applied physiology》2003,95(4):1575-1583
Occupational or recreational exercise reduces mortality from cardiovascular disease. The potential mechanisms for this reduction may include changes in blood pressure (BP) and autonomic control of the circulation. Therefore, we conducted the present long-term longitudinal study to quantify the dose-response relationship between the volume and intensity of exercise training, and regulation of heart rate (HR) and BP. We measured steady-state hemodynamics and analyzed dynamic cardiovascular regulation by spectral and transfer function analysis of cardiovascular variability in 11 initially sedentary subjects during 1 yr of progressive endurance training sufficient to allow them to complete a marathon. From this, we found that 1) moderate exercise training for 3 mo decreased BP, HR, and total peripheral resistance, and increased cardiovascular variability and arterial baroreflex sensitivity; 2) more prolonged and intense training did not augment these changes further; and 3) most of these changes returned to control values at 12 mo despite markedly increased training duration and intensity equivalent to that routinely observed in competitive athletes. In conclusion, increases in R-wave-R-wave interval and cardiovascular variability indexes are consistent with an augmentation of vagal modulation of HR after exercise training. It appears that moderate doses of training for 3 mo are sufficient to achieve this response as well as a modest hypotensive effect from decreasing vascular resistance. However, more prolonged and intense training does not necessarily lead to greater enhancement of circulatory control and, therefore, may not provide an added protective benefit via autonomic mechanisms against death by cardiovascular disease. 相似文献