全文获取类型
收费全文 | 7920篇 |
免费 | 555篇 |
国内免费 | 3篇 |
出版年
2023年 | 37篇 |
2022年 | 30篇 |
2021年 | 180篇 |
2020年 | 101篇 |
2019年 | 144篇 |
2018年 | 182篇 |
2017年 | 157篇 |
2016年 | 211篇 |
2015年 | 393篇 |
2014年 | 424篇 |
2013年 | 607篇 |
2012年 | 660篇 |
2011年 | 604篇 |
2010年 | 384篇 |
2009年 | 315篇 |
2008年 | 501篇 |
2007年 | 452篇 |
2006年 | 420篇 |
2005年 | 384篇 |
2004年 | 349篇 |
2003年 | 346篇 |
2002年 | 312篇 |
2001年 | 62篇 |
2000年 | 46篇 |
1999年 | 72篇 |
1998年 | 77篇 |
1997年 | 51篇 |
1996年 | 56篇 |
1995年 | 50篇 |
1994年 | 52篇 |
1993年 | 36篇 |
1992年 | 49篇 |
1991年 | 36篇 |
1990年 | 43篇 |
1989年 | 43篇 |
1988年 | 27篇 |
1987年 | 33篇 |
1986年 | 39篇 |
1985年 | 26篇 |
1984年 | 50篇 |
1983年 | 26篇 |
1982年 | 37篇 |
1980年 | 28篇 |
1979年 | 40篇 |
1977年 | 24篇 |
1976年 | 26篇 |
1975年 | 33篇 |
1974年 | 24篇 |
1971年 | 22篇 |
1970年 | 22篇 |
排序方式: 共有8478条查询结果,搜索用时 421 毫秒
1.
2.
3.
Hanna G. Budayeva Arundhati Sengupta-Ghosh Lilian Phu John G. Moffat Gai Ayalon Donald S. Kirkpatrick 《Molecular & cellular proteomics : MCP》2022,21(4):100221
Muscle-specific receptor tyrosine kinase (MuSK) agonist antibodies were developed 2 decades ago to explore the benefits of receptor activation at the neuromuscular junction. Unlike agrin, the endogenous agonist of MuSK, agonist antibodies function independently of its coreceptor low-density lipoprotein receptor–related protein 4 to delay the onset of muscle denervation in mouse models of ALS. Here, we performed dose–response and time-course experiments on myotubes to systematically compare site-specific phosphorylation downstream of each agonist. Remarkably, both agonists elicited similar intracellular responses at known and newly identified MuSK signaling components. Among these was inducible tyrosine phosphorylation of multiple Rab GTPases that was blocked by MuSK inhibition. Importantly, mutation of this site in Rab10 disrupts association with its effector proteins, molecule interacting with CasL 1/3. Together, these data provide in-depth characterization of MuSK signaling, describe two novel MuSK inhibitors, and expose phosphorylation of Rab GTPases downstream of receptor tyrosine kinase activation in myotubes. 相似文献
4.
Jin Wei Mia Madel Alfajaro Peter C. DeWeirdt Ruth E. Hanna William J. Lu-Culligan Wesley L. Cai Madison S. Strine Shang-Min Zhang Vincent R. Graziano Cameron O. Schmitz Jennifer S. Chen Madeleine C. Mankowski Renata B. Filler Neal G. Ravindra Victor Gasque Fernando J. de Miguel Ajinkya Patil Huacui Chen Craig B. Wilen 《Cell》2021,184(1):76-91.e13
5.
6.
7.
8.
Giovanni Murtas 《Systems and synthetic biology》2010,4(2):85-93
One of the major properties of the semi-synthetic minimal cell, as a model for early living cells, is the ability to self-reproduce
itself, and the reproduction of the boundary layer or vesicle compartment is part of this process. A minimal bio-molecular
mechanism based on the activity of one single enzyme, the FAS-B (Fatty Acid Synthase) Type I enzyme from Brevibacterium ammoniagenes, is encapsulated in 1-palmitoyl-2oleoyl-sn-glycero-3-phosphatidylcholine (POPC) liposomes to control lipid synthesis. Consequently molecules of palmitic acid released
from the FAS catalysis, within the internal lumen, move toward the membrane compartment and become incorporated into the phospholipid
bilayer. As a result the vesicle membranes change in lipid composition and liposome growth can be monitored. Here we report
the first experiments showing vesicles growth by catalysis of one enzyme only that produces cell boundary from within. This
is the prototype of the simplest autopoietic minimal cell. 相似文献
9.
Sequence complexity and diversity of polyadenylated RNA molecules transcribed in human myeloid cells
10.
Sergio Davinelli Mariano Intrieri Claudio Russo Alfonso Di Costanzo Davide Zella Paolo Bosco Giovanni Scapagnini 《Immunity & ageing : I & A》2011,8(1):1-10
Alzheimer's disease is a progressive and neurodegenerative disorder which involves multiple molecular mechanisms. Intense research during the last years has accumulated a large body of data and the search for sensitive and specific biomarkers has undergone a rapid evolution. However, the diagnosis remains problematic and the current tests do not accurately detect the process leading to neurodegeneration. Biomarkers discovery and validation are considered the key aspects to support clinical diagnosis and provide discriminatory power between different stages of the disorder. A considerable challenge is to integrate different types of data from new potent approach to reach a common interpretation and replicate the findings across studies and populations. Furthermore, long-term clinical follow-up and combined analysis of several biomarkers are among the most promising perspectives to diagnose and manage the disease. The present review will focus on the recent published data providing an updated overview of the main achievements in the genetic and biochemical research of the Alzheimer's disease. We also discuss the latest and most significant results that will help to define a specific disease signature whose validity might be clinically relevant for future AD diagnosis. 相似文献