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1.
A seasonal migration in the hermit crab Clibanarius vittatus (Bosc) is described and its structure examined for size-related or sexual differences that might lead to differential mortalities. The largest crabs, which were nearly all males, began to leave the shore in early summer, but the smaller females stayed until late autumn. The smaller crabs also returned to the shore at least one month in advance of the large crabs in the spring. No size-related or sexual differences were found in the abilities of the crabs to regulate their volumes when moved from 34.5%. to 10.5%. salinity which is the observed salinity range in their usual habitat. 相似文献
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Jan-Ingmar Flock Ian Fotheringham Janice Light Les Bell Roger Derbyshire 《Molecular & general genetics : MGG》1984,195(1-2):246-251
Summary A chemically synthesised gene coding for human urogastrone which was earlier cloned in E. coli (Smith et al. 1982) has now been cloned into expression vectors for Bacillus subtilis
Two types of constructs have been made, one giving production of methionylurogastrone and the other giving rise to a methionyl-urogastrone- galactosidase fusion polypeptide facilitating quantification of expression levels.The ribosome binding sites used in the expression plasmids are synthetically made oligonucleotides residing on short restriction fragments to allow easy replacement by other ribosome binding sites.Using shuttle vectors and constitutive promoters from Bacillus phages 105 and SPP1, we were able to detect levels of expression amounting to a few thousand molecules per cell during logarithmic growth in both E. coli and B. subtilis. 相似文献
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J J?ger E K?hler P Tucker U Sauder Z Housley-Markovic I Fotheringham M Edwards M Hunter K Kirschner J N Jansonius 《Journal of molecular biology》1989,209(3):499-501
Mutant aspartate aminotransferase V39L (Val39 replaced by Leu) from Escherichia coli has been crystallized into a monoclinic cell from a polyethylene glycol solution (pH 7.5) by vapor diffusion. The space group and the unit cell dimensions have been determined using a precession camera, a CAD4 diffractometer and a Nicolet Xentronics area detector to be P2(1) with a = 86.8 A, b = 79.9 A, c = 89.4 A, beta = 118.74 degrees. The crystals diffract to better than 2.3 A and are suitable for X-ray structure analysis. 相似文献
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He A Li T Daniels L Fotheringham I Rosazza JP 《Applied and environmental microbiology》2004,70(3):1874-1881
We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had K(m) values for benzoate, ATP, and NADPH that were similar to those for natural CAR, and recombinant CAR reduced benzoic, vanillic, and ferulic acids to their corresponding aldehydes. car is the first example of a new gene family encoding oxidoreductases with remote acyl adenylation and reductase sites. 相似文献
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Fotheringham J Xu FY Nemer M Kardami E Choy PC Hatch GM 《Biochimica et biophysica acta》2000,1481(1):1-10
This work attempts to explain several aspects of the response of plasminogen to 6-aminohexanoate (6-AH). These responses include the overall fluorescent changes that occur when plasminogen binds the ligand, the changes shown by the individual domains when they bind the ligand, and the changes in structure shown by the holoprotein when it binds 6-AH. The results have implications for understanding the physicochemical behavior of all kringle based proteins. 相似文献
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Epstein-Barr virus latent membrane protein 2A (LMP2A) induces many characteristics of carcinoma, including transformation, migration, invasion, and impaired differentiation. The MCF10A cell line differentiates to form hollow acini when grown in Matrigel, and expression of LMP2A inhibited differentiation and anoikis induced by loss of matrix attachment. LMP2A-infected cells formed large, lobular structures rather than hollow acini. Autophagy inhibitors impaired this abnormal growth and induced caspase 3 activation and acinus formation. LMP2A also increased autophagosome formation and expression of proteins in the autophagosome pathway. These findings suggest that LMP2A may inhibit anoikis and luminal clearance in acini through induction of autophagy. 相似文献
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Oil removal from used sorbents using a biosurfactant 总被引:3,自引:0,他引:3
Oil spills impose serious damage on the environment. Mechanical recovery by the help of oil sorbents is one of the most important countermeasures in oil spill response. Most sorbents, however, end up in landfills or in incineration after a single use. These options either produce another source of pollution or increase the oil recovery cost. In this study a biosurfactant was used to clean used oil sorbents. This use of biosurfactants is new. Washing parameters tested included sorbent type, washing time, surfactant dosage and temperature. It was found that with biosurfactant washing more than 95% removal of the oil from sorbents was achieved, depending on the washing conditions. Biosurfactants were found to have considerable potential for recycling the used sorbents. 相似文献