排序方式: 共有13条查询结果,搜索用时 46 毫秒
1.
Mahsa Javadian Tohid Gharibi Najibeh Shekari Meghdad Abdollahpour-Alitappeh Ali Mohammadi Arezoo Hossieni Hamed Mohammadi Tohid Kazemi 《Journal of cellular physiology》2019,234(5):5399-5412
MicroRNAs (miRNAs) regulate several biological and physiological processes in mammalian cells, including cellular proliferation, differentiation, apoptosis, and metabolism. Recent studies have confirmed the alteration of them during the cancer development. Matrix metalloproteinases (MMPs), belonging to the large family of proteases, have also been demonstrated to play crucial roles in tissue remodeling, and to support cancer progression and metastasis. There are several known miRNAs which regulate the MMP family and their expression. The expression profiles of miRNAs involved in MMP regulation, change during cancer progression, and metastasis. The present review focuses on important miRNAs capable of targeting MMPs through direct and indirect interactions during the breast cancer development, progression, and metastasis. 相似文献
2.
David K. Clarke Farooq Nasar Siew Chong J. Erik Johnson John W. Coleman Margaret Lee Susan E. Witko Cheryl S. Kotash Rashed Abdullah Shakuntala Megati Amara Luckay Becky Nowak Andrew Lackner Roger E. Price Peter Little Narender Kalyan Valerie Randolf Ali Javadian Timothy J. Zamb Christopher L. Parks Michael A. Egan John Eldridge Michael Hendry Stephen A. Udem 《Journal of virology》2014,88(12):6690-6701
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N. Javadian G. Karimzadeh S. Mahfoozi F. Ghanati 《Russian Journal of Plant Physiology》2010,57(4):540-547
Quantitative changes in total leaf soluble proteins, proline, carbohydrate content, chlorophyll fluorescence, guaiacol peroxidase
(POD) and catalase (CAT) activities were determined in a less cold-hardy (LCH) spring cv. Kohdasht (LT50 = −6°C), a semi cold-hardy (SCH) facultative cv. Azar 2 (LT50 = −15°C), and a cold-hardy (CH) winter cv. Norstar (LT50 = −26°C) of wheat (Triticum aestivum L.) exposed to 4°C for 9 weeks. Seedlings were grown in a controlled growth room for 14 days at 20°C and then transferred
to 4°C (experimental day 0) for 63 days (cold treatment); otherwise they were maintained continuously at 20°C (control treatment).
The samples were harvested 0, 2, 21, 28, 42, and 63 days after exposure to 4°C. The results showed significant low temperature
(LT)-induced accumulation of total soluble proteins, proline, and carbohydrates and elevation in activities of CAT and POD
in leaves of SCH and CH winter cultivars rather than in LCH spring cultivar. In contrast, the chlorophyll fluorescence (F
v/F
m) declined during LT treatment irrespective of cultivar. The results suggest that developmental traits such as vernalization
requirement of wheat affects on cold-tolerance expression system of plants. 相似文献
4.
Mahsa Javadian Najibeh Shekari Mohammad S. Soltani - Zangbar Ali Mohammadi Behzad Mansoori Sepideh Maralbashi Dariush Shanehbandi Behzad Baradaran Masood Darabi Tohid Kazemi 《Journal of cellular biochemistry》2020,121(3):2416-2427
There is insufficient evidence with respect to the effect of the standard anticancer therapeutic agents as well as common dietary supplements on the expression of such genes and microRNAs (miRNAs). Therefore, this study was aimed to study the effect of applying linoleic acid (LA) and docosahexaenoic acid (DHA) fatty acids alone or combined with Taxol on the expression of the matrix metalloproteinase (MMP)-9, MMP-2, vimentin, and talin2 genes, tumor-suppressor miR-194 and, onco-miR-106b in triple-negative breast cancer cell line, known as MDA-MB-231. MDA-MB-231 as metastatic breast cancer cell line was cultured and treated using 0.3 μM Taxol, 100 μM DHA, and 50 μM LA for 24 hours, alone or combined with Taxol under the normoxic and hypoxic conditions. Cells were harvested, after RNA extraction and complementary DNA synthesis, analysis of the expression levels of the studied genes and miRNAs was done through the use of the quantitative real-time polymerase chain reaction (qRT-PCR). Wound healing assay and Western blot analysis were also performed for confirmation. The results of qRT-PCR showed that treating the MDA-MB-231 cells with DHA caused an increase in the miR-194 expression and a decrease in the miR-106b expression, leading to the downregulation of the MMP-2 and MMP-9, and vimentin, and upregulation of the talin2 under the normoxic and hypoxic conditions. The results of the wound healing scratch assay revealed that the administration of the DHA and the DHA-Taxol combination caused the repression of cell migration in comparison with the control groups under the normoxic and hypoxic conditions. The results of the Western blot analysis demonstrated that DHA and the DHA-Taxol combination caused an increase in the expression of the talin2 protein rather than the control cells under both normoxic and hypoxic conditions. This study showed that DHA has significant antimetastatic effects against the triple-negative breast cancer cells. DHA could serve as a promising supplementation for suppressing the breast cancer cell migration, especially under the hypoxic condition. 相似文献
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6.
S. Tahmasebi Boroujeni N. Naghdi M. Shahbazi A. Farrokhi F. Bagherzadeh A. Kazemnejad M. Javadian 《Biological trace element research》2009,130(1):48-61
Zinc deficiency during pregnancy and during lactation has been shown to impair cognitive function and motor activity in offspring
rats. In the present study, the effect of zinc deficiency and zinc supplement on spatial learning and memory in Morris Water
Maze (MWM) and motor activity in open field were investigated. Pregnant rats after mating were divided to three groups. Control
group fed a standard diet and a zinc deficient (ZnD) group fed a diet deficient in zinc (0.5–1.5 ppm) and a zinc supplement
(ZnS) group fed a standard diet and enhanced zinc in the drinking water (10 ppm). All the diets were exposed during the last
trisemester of pregnancy and during lactation. Rat’s offspring in these groups were tested for spatial learning and memory
in MWM at post natal day (PND) 56 and were tested for motor activity in open field at PND 66.The Escape Latency (EL) and Traveled
Distance (TD) in the ZnD group were increased but Percentage of Time Spent in the target quadrant (PTS) was decreased compared
to the control group. In addition, these were no significant differences in EL and TD, but PTS had significant increase in
ZnS compared to the control group. In the open field, Total Distance Moved (TDM) and Time of Motor Activity (TMA) for the
ZnD were decreased compared to the control group, but there were no significant differences in TDM and TMA between control
and ZnS groups. These findings suggest that zinc deficiency during the last trimester of pregnancy and during lactation impaired
spatial learning and memory in their offsprings and has also negative effect on motor activity. In addition, ZnS has a significant
effect on spatial learning and memory but no effect on motor activity in their offsprings. 相似文献
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8.
Resistance of previously infected chimpanzees to successive challenges with a heterologous intraclade B strain of human immunodeficiency virus type 1. 总被引:2,自引:2,他引:0 下载免费PDF全文
R Shibata C Siemon M W Cho L O Arthur S M Nigida Jr T Matthews L A Sawyer A Schultz K K Murthy Z Israel A Javadian P Frost R C Kennedy H C Lane M A Martin 《Journal of virology》1996,70(7):4361-4369
To test whether the protective effects of attenuated simian immunodeficiency virus vaccines in macaques were applicable to the human immunodeficiency virus type 1 (HIV-1)-chimpanzee system, two groups of animals, previously infected with HIV-1(IIIB) or HIV-1(SF2) were each challenged with a heterologous clade B virus, HIV-1(DH12). Following challenge, the parameters measured included virus isolation (from plasma, peripheral blood mononuclear cells, and lymph node tissue); quantitative DNA PCR using primers capable of distinguishing HIV-1(IIIB), HIV-1(SF2), and HIV-1(DH12) from one another; and serologic assays to monitor changes in binding and neutralizing antibodies. In contrast to an HIV-1-naive chimpanzee that rapidly became infected following the inoculation of HIV-1(DH12), the two chimpanzees previously infected with HIV-1(IIIB) resisted repeated and escalating inoculations of HIV-1(DH12), as monitored by virus isolation and PCR. The two animals previously infected with HIV-1(SF2) became infected with HIV-1(DH12) but in contrast to the case with the HIV-1-naive chimpanzee, no cell-free viral RNA was detected in the plasma by the branched DNA procedure and levels of peripheral blood mononuclear cell-associated viral DNA were reduced 35- to 50-fold. 相似文献
9.
Jean D. Boyer Bin Wang Kenneth E. Ugen Michael Agadjanyan Ali Javadian Patrice Frost Kesen Dang Richard A. Carrano Richard Ciccarelli Leslie Coney William V. Williams David B. Weiner 《Journal of medical primatology》1996,25(3):242-250
Abstract: An effective immune response involves the specific recognition of and elimination of an infectious organism at multiple levels. In this context DNA immunization can present functional antigenic proteins to the host for recognition by all arms of the immune system, yet provides the opportunity to delete any genes of the infectious organism which code for antigens or pieces of antigens that may have deleterious effects. Our group has developed the use of nucleic acid immunization as a possible method of vaccination against Human immunodeficiency virus type 1 (HIV-1) [1,2,3,10,11,12]. Sera from non-human primates immunized with DNA vectors that express the envelope proteins from HIV-1 contain antibodies specific to the HIV-1 envelope. These sera also neutralize HIV-1 infection in vitro and inhibit cell to cell infection in tissue culture. Analysis of cellular responses is equally encouraging. T cell proliferation as well as cytotoxic T cell lysis of relevant env expressing target cells were observed. In addition, evidence that DNA vaccines are capable of inducing a protective response against live virus was demonstrated using a chimeric SIV/HIV (SHIV) challenge in vaccinated cynomologous macaques. We found that nucleic acid vaccination induced protection from challenge in one out of four immunized cynomolgus macaques and viral load was lower in the vaccinated group of animals versus the control group of animals. These data encouraged us to analyze this vaccination technique in chimpanzees, the most closely related animal species to man. We observed the induction of both cellular and humoral immune responses with a DNA vaccine in chimpanzees. These studies demonstrate the utility of this technology to induce relevant immune responses in primates which may ultimately lead to effective vaccines. 相似文献
10.
Javadian Neda Karimzadeh Ghasem Sharifi Mohsen Moieni Ahmad Behmanesh Mehrdad 《Planta》2017,245(6):1165-1178
Planta - Induction of tetraploidy was performed and podophyllotoxin production increased by upregulating the expression level and enzyme activity of genes related to its biosynthesis in tetraploid... 相似文献