The 16S-23S intergenic spacer region (spacer region 1) of Streptococcus salivarius, S. thermophilus, and Lactococcus lactis subsp. cremoris and the 23S-5S intergenic spacer region (spacer region 2) of S. salivarius and L. lactis subsp. cremoriswere sequenced and compared with the spacer regions 1 and 2 of other streptococci. A high degree of intraspecific conservation was observed for S. thermophilus and L. lactis, and very similar sequences were found for S. salivarius and S. thermophilus. Whereas spacer region 1 is highly conserved in the genus Streptococcus sensu-stricto,only the tRNA gene and the rRNA processing stems are highly conserved in the three genera: Streptococcussensu-stricto, Lactococcus, and Enterococcus. The presence of a unique tRNAAla gene without the 3 terminal CCA sequence seems to be a general feature of the streptococci spacer region 1. A secondary structure model was built to show the interaction between the spacer regions 1 and 2 of S. thermophilus and S. salivarius. The rapid evolution of spacer region 1 in streptococci is in part due to insertions and deletions of small RNA stem/loop structures. 相似文献
Insulin degrading enzyme (IDE) is a thiol-dependent metalloendoprotease that is responsible for initiation of cellular insulin degradation. However, its exact mode of action and the factors controlling it are poorly understood. Since IDE is a metal requiring enzyme, we have examined which metal(s) is(are) endogenously associated with it. Using neutron activation analysis, we studied the metal content of a partially purified enzyme from three different tissues: rat skeletal muscle, rat liver, and human placenta. Our results indicate that zinc and manganese are associated with the enzyme with approximately 10 times more zinc as manganese being present. These results suggest that one or both of these two metals are endogenously associated with this enzyme and are a means of controlling the enzyme's activity. 相似文献
Plant Cell, Tissue and Organ Culture (PCTOC) - Globular androgenic haploid embryos of TV21 and TV19 cultivars of Camellia ssp., obtained on embryo induction medium (EIM), Murashige and Skoog medium... 相似文献
Molecular Biology Reports - Peri-implantitis (PI) is a multifactorial condition caused by the interactions of pathogens and the host immune response. Previous studies have demonstrated a... 相似文献
Journal of Plant Growth Regulation - Plants require optimum amounts of nutrients for suitable growth and yield production. Accordingly, the most efficient methods of fertilization, including the... 相似文献
Testing and developing nutrient diagnosis methods, which can result in the optimum production of fruits, is of significance. The nutritional balance and requirements of almond (Prunus sp.) orchards, in the city of Saman (province of Chaharmahal and Bakhtiari, Iran, one of the biggest producers of almond in the country), were investigated using the methods of diagnosis and recommendation integrated system (DRIS) and deviation from optimum percentage (DOP) in a two-year research. Using 36 gardens along the Zayandehrud River in a 60-km distance, soil physical and chemical properties, as well as leaf micro- and macro-nutrient contents were determined. Similar plant samples (leaf + petiole) in terms of age, genotype and rootstock were collected from the unfruitful trees. The most deficient nutrients including S, Cu, Zn and Mn were indicated by the DRIS and DOP methods. However, according to the DRIS method Mg, and according to the DOP method N, K and Mg were determined as the most excessive macronutrients. Interestingly, both methods indicated Mo as the most excessive micronutrient. The balance indexes of different nutrients for different orchards indicated that the nutritional balances of the orchards from the highest to the least deficiency are according to the following order Cu > S > Zn > Mn > Cl > P > Ca > Mg > B > N > K > Fe > Mo. Such results indicate the precisions and some similarities between the two methods. However, the two methods were compared using SAS Proc GLM, Proc REG, and Proc NLIN. The analyses indicated that the two methods were significantly different and the DOP method (significant model) indicated higher correlation with the results. Accordingly, the DOP method may be a more accurate method of estimating almond yield as affected by the concentration of different nutrients. It is possible to determine the deficiency, balance and excessiveness of nutrients in almond orchards using the DRIS and DOP methods, which is of economic and environmental significance, worldwide.
Several studies have focused on the RAGE genetic background and have demonstrated that its polymorphisms affect the receptor's activity, expression, and downstream signaling. However, there is only little information regarding RAGE polymorphism in breast cancer. In the present study, the authors studied RAGE polymorphisms in 71 patients with breast cancer and 93 healthy women. RAGE –374T/A, –429T/C, and 63 bp Ins/del polymorphisms were analyzed using a hexaprimer amplification refractory mutation system PCR (H-ARMS-PCR). The results showed that RAGE polymorphisms are not associated with breast cancer in the current study population. Larger studies are required to confirm these data in other populations. 相似文献