全文获取类型
收费全文 | 976篇 |
免费 | 127篇 |
出版年
2021年 | 8篇 |
2018年 | 10篇 |
2017年 | 6篇 |
2016年 | 17篇 |
2015年 | 29篇 |
2014年 | 28篇 |
2013年 | 31篇 |
2012年 | 53篇 |
2011年 | 39篇 |
2010年 | 36篇 |
2009年 | 22篇 |
2008年 | 39篇 |
2007年 | 51篇 |
2006年 | 50篇 |
2005年 | 32篇 |
2004年 | 43篇 |
2003年 | 46篇 |
2002年 | 39篇 |
2001年 | 35篇 |
2000年 | 23篇 |
1999年 | 23篇 |
1998年 | 12篇 |
1997年 | 11篇 |
1996年 | 8篇 |
1995年 | 15篇 |
1994年 | 9篇 |
1993年 | 13篇 |
1992年 | 20篇 |
1991年 | 12篇 |
1990年 | 24篇 |
1989年 | 15篇 |
1988年 | 11篇 |
1987年 | 16篇 |
1986年 | 11篇 |
1985年 | 11篇 |
1984年 | 14篇 |
1983年 | 9篇 |
1982年 | 13篇 |
1981年 | 7篇 |
1980年 | 10篇 |
1979年 | 8篇 |
1978年 | 8篇 |
1977年 | 14篇 |
1971年 | 5篇 |
1965年 | 7篇 |
1935年 | 8篇 |
1932年 | 5篇 |
1931年 | 6篇 |
1930年 | 7篇 |
1929年 | 11篇 |
排序方式: 共有1103条查询结果,搜索用时 475 毫秒
1.
Direct evidence is presented for a proline cycle using a cell-free experimental system which sequentially transfers 3H from [1-3H]glucose to NADP+ to Δ1-pyrroline-5-carboxylate and yields [3H]proline. The formation of [3H]proline depends on the presence of NADP, Δ1-pyrroline-5-carboxylate, and the enzymes glucose-6-phosphate dehydrogenase and Δ1-pyrroline-5-carboxylate reductase. The production of [3H]proline from unlabeled proline in the presence of mitochondria provides direct evidence for one complete turn of a proline cycle which transfers reducing equivalents produced by glucose oxidation in the pentose pathway into mitochondria. In this cycle, proline is oxidized to Δ1-pyrroline-5-carboxylate by mitochondrial proline oxidase. Δ1-pyrroline-5-carboxylate is released from mitochondria and is recycled back to proline by Δ1-pyrroline-5-carboxylate reductase with concomitant oxidation of NADPH. At the maximal rate observed, 60% of Δ1-pyrroline-5-carboxylate produced is recycled back to proline. This cycle provides a mechanism for transferring reducing equivalents from NADPH into mitochondria and is linked to glucose oxidation in the pentose pathway by NADPH turnover. 相似文献
2.
3.
Curt Herbst 《Development genes and evolution》1906,22(4):473-497
Ohne Zusammenfassung 相似文献
4.
C. W. Forsberg 《Applied microbiology》1980,39(2):453-455
Two rumen nitrate-reducing isolates of Desulfovibrio desulfuricans were found to hydrolyze cysteine with the production of sulfide and pyruvate. When cultured on agar medium containing yeast extract with nitrate as the primary electron acceptor and ferrous chloride as the indicator, blackening of colonies occurred. The blackening of colonies appeared sooner and was more intense when either cysteine or sulfate was added to the culture medium with nitrate present. 相似文献
5.
Curt Koßwig 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1932,4(1):22-32
Ohne Zusammenfassung 相似文献
6.
An enzyme which released the cellobiose group from p-nitrophenyl cellobioside was isolated from the periplasmic space of Bacteroides succinogenes grown on Avicel crystalline cellulose in a continuous cultivation system and separated from endoglucanases by column chromatography. The molecular weight of the enzyme was approximately 40,000, as estimated by gel filtration. The enzyme has an isoelectric point of 4.9. The enzyme exhibited low hydrolytic activity on acid-swollen cellulose and practically no activity on carboxymethyl cellulose, Avicel cellulose, and cellobiose, but it hydrolyzed p-nitrophenyl lactoside and released cellobiose from cellotriose and from higher cello-oligosaccharides. These data demonstrate that the enzyme is a cellodextrinase with an exotype of function. 相似文献
7.
Molecular cloning and expression in Escherichia coli of a cellodextrinase gene from Bacteroides succinogenes S85 总被引:1,自引:0,他引:1
A DNA fragment coding for a cellodextrinase of Bacteroides succinogenes S85 was isolated by screening of a pBR322 gene library in Escherichia coli HB101. Of 100,000 colonies screened on a complex medium with methylumbelliferyl-beta-D-cellobioside as the indicator substrate, two cellodextrinase-positive clones (CB1 and CB2) were isolated. The DNA inserts from the two recombinant plasmids were 7.7 kilobase pairs in size and had similar restriction maps. After subcloning from pCB2, a 2.5-kilobase-pair insert which coded for cellodextrinase activity was isolated. The enzyme was located in the cytoplasm of the E. coli host. It exhibited no activity on carboxymethyl cellulose, Avicel microcrystalline cellulose, acid-swollen cellulose, or cellobiose but hydrolyzed p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-lactoside. The Km (0.1 mM) for the hydrolysis of p-nitrophenyl-cellobioside by the enzyme expressed in E. coli was similar to that reported for the purified enzyme from B. succinogenes. Expression of the cellodextrinase gene was subjected to catabolite repression by glucose and was not induced by cellobiose. The origin of the DNA insert from B. succinogenes was confirmed by Southern blot analysis. Western blotting (immunoblotting) using antibodies raised against the purified B. succinogenes cellodextrinase revealed a protein with a molecular weight of approximately 50,000 in E. coli clones which comigrated with the native enzyme isolated from B. succinogenes. These data indicate that the cellodextrinase gene expressed in E. coli is fully functional and codes for an enzyme with properties similar to those of the native enzyme. 相似文献
8.
Molecular cloning and expression in Escherichia coli of a cellodextrinase gene from Bacteroides succinogenes S85. 总被引:4,自引:0,他引:4 下载免费PDF全文
A DNA fragment coding for a cellodextrinase of Bacteroides succinogenes S85 was isolated by screening of a pBR322 gene library in Escherichia coli HB101. Of 100,000 colonies screened on a complex medium with methylumbelliferyl-beta-D-cellobioside as the indicator substrate, two cellodextrinase-positive clones (CB1 and CB2) were isolated. The DNA inserts from the two recombinant plasmids were 7.7 kilobase pairs in size and had similar restriction maps. After subcloning from pCB2, a 2.5-kilobase-pair insert which coded for cellodextrinase activity was isolated. The enzyme was located in the cytoplasm of the E. coli host. It exhibited no activity on carboxymethyl cellulose, Avicel microcrystalline cellulose, acid-swollen cellulose, or cellobiose but hydrolyzed p-nitrophenyl-beta-D-cellobioside and p-nitrophenyl-beta-D-lactoside. The Km (0.1 mM) for the hydrolysis of p-nitrophenyl-cellobioside by the enzyme expressed in E. coli was similar to that reported for the purified enzyme from B. succinogenes. Expression of the cellodextrinase gene was subjected to catabolite repression by glucose and was not induced by cellobiose. The origin of the DNA insert from B. succinogenes was confirmed by Southern blot analysis. Western blotting (immunoblotting) using antibodies raised against the purified B. succinogenes cellodextrinase revealed a protein with a molecular weight of approximately 50,000 in E. coli clones which comigrated with the native enzyme isolated from B. succinogenes. These data indicate that the cellodextrinase gene expressed in E. coli is fully functional and codes for an enzyme with properties similar to those of the native enzyme. 相似文献
9.
Factors affecting adhesion of Fibrobacter succinogenes subsp. succinogenes S85 and adherence-defective mutants to cellulose. 总被引:4,自引:4,他引:0 下载免费PDF全文
Fibrobacter succinogenes subsp. succinogenes S85, formerly Bacteroides succinogenes, adheres to crystalline cellulose present in the culture medium. When the cells are suspended in buffer, adhesion is enhanced by increasing the ionic strength. Heat, glutaraldehyde, trypsin, and pronase treatments markedly reduce the extent of adhesion. Treatment with dextrinase, modification of amino and carboxyl groups with Formalin or other chemical agents, and inclusion of either albumin (1%) or Tween 80 (0.5%) do not decrease the degree of adhesion. Adherence-defective mutants isolated by their inability to bind to cellulose exhibited different growth characteristics. Class 1 mutants grew on glucose, cellobiose, amorphous cellulose, and crystalline cellulose. Class 3 mutants grew on glucose and cellobiose but not on amorphous or crystalline cellulose. No substantial changes were detected in the endoglucanase, cellobiosidase, and cellobiase activities of the wild type and the mutants. These data suggest that adhesion to crystalline cellulose is specific and that it involves surface proteins. 相似文献
10.