A Multicenter Study of Viral Aetiology of Community-Acquired Pneumonia in Hospitalized Children in Chinese Mainland

Virologica Sinica - Community-acquired pneumonia (CAP) is one of the leading causes of morbidity and mortality in children worldwide. In this study, we aimed to describe the aetiology of viral...     

Monitoring the biology stability of human umbilical cord-derived mesenchymal stem cells during long-term culture in serum-free medium

Mesenchymal stem cells (MSCs) are multipotent adult stem cells that have an immunosuppressive effect. The biological stability of MSCs in serum-free medium during long-term culture in vitro has not been elucidated clearly. The morphology, immunophenotype and multi-lineage potential were analyzed at passages 3, 5, 10, 15, 20, and 25 (P₃, P₅, P₁₀, P₁₅, P₂₀, and P₂₅, respectively). The cell cycle distribution, apoptosis, and karyotype of human umbilical cord-derived (hUC)-MSCs were analyzed at P₃, P₅, P₁₀, P₁₅, P₂₀, and P₂₅. From P₃ to P₂₅, the three defining biological properties of hUC-MSCs [adherence to plastic, specific surface antigen expression, multipotent differentiation potential] met the standards proposed by the International Society for Cellular Therapy for definition of MSCs. The cell cycle distribution analysis at the P₂₅ showed that the percentage of cells at G0/G1 was increased, compared with the cells at P₃ (P < 0.05). Cells at P₂₅ displayed an increase in the apoptosis rate (to 183 %), compared to those at P₃ (P < 0.01). Within subculture generations 3–20 (P₃–P₂₀), the differences between the cell apoptotic rates were not statistically significant (P > 0.05). There were no detectable chromosome eliminations, displacements, or chromosomal imbalances, as assessed by the karyotyping guidelines of the International System for Human Cytogenetic Nomenclature (ISCN, 2009). Long-term culture affects the biological stability of MSCs in serum-free MesenCult-XF medium. MSCs can be expanded up to the 25th passage without chromosomal changes by G-band. The best biological activity period and stability appeared between the third to 20th generations.     

A multi-center study on Molecular Epidemiology of Human Respiratory Syncytial Virus from Children with Acute Lower Respiratory Tract Infections in the Mainland of China between 2015 and 2019

Virologica Sinica - Human respiratory syncytial virus (RSV) is a major pathogen of acute lower respiratory tract infection among young children. To investigate the prevalence and genetic...     

广西14市猪戊型肝炎的分子流行病学分析

为调查广西猪戊型肝炎(HE)的感染情况,本研究应用套式RT-PCR方法对采自广西14市43个猪场的508份3月龄左右猪只粪样及市售182份猪肝扩增猪戊型肝炎病毒(HEV)ORF2基因特异序列,并进行核苷酸序列同源性分析及遗传进化分析。结果表明,在508份猪粪样中194份可以检测到HEV RNA,阳性率为38.2%;182份猪肝样本中,33份可检测到HEV RNA,阳性率为18.1%;43个猪场中检出HEV RNA的有35个,猪场阳性率为81.4%。所获取的39株广西猪HEV ORF2基因特异序列同源性为89.9%～100%,提示在所调查的广西猪场中已较普遍存在HEV感染,所获取的39株广西HEV阳性样品均属于基因Ⅳ型。本研究结果佐证了感染猪及市售带毒猪肝脏是HEV重要的储存库和传染源,应该重视其公共卫生学意义,预防粪-口途径或者食源性HEV感染。     

Length–weight relationship of three fish species from the Qiantang River,China

Length–weight relationships (LWRs) of the three fish species, Pseudobagrus albomarginatus(Rendahl, 1928), Pseudobagrus crassilabris (Günther, 1864), and Rhinogobius davidi(Sauvage & Dabry de Thiersant, 1874), were determined respectively. Samples were collected from Qiantang River, China during March 2016–December 2017. The bvalues ranged from 2.525 to 2.917, the coefficient of determination (r²) ranged from 0.96 to 0.97. Prior to this study, the length–weight relationship for P. albomarginatus and R. davidi were unknown to FishBase and the new maximum total lengths for three species were not recorded in FishBase. These results are useful for the fishery research and management in Qiantang River.     

鲸豚类的渗透调节研究进展

鲸豚类作为哺乳动物长期演化中较为特殊的一支,它们所有的生命活动都在水中完成,所以它们在身体结构、生理机能以及生态习性等方面都发展形成了适用于水中生活的完善的适应策略。大部分鲸豚类动物生活在海洋中,只有少部分生活在淡水里,但不管是生活在高渗环境中的海洋鲸类还是生活在低渗环境中的淡水鲸类,维持水盐平衡以及渗透压的内稳态是它们所面临的一个共同问题。鲸豚类究竟是通过怎样的渗透调节机制来实现对不同渗透环境的适应呢?它们在身体结构、生理调节和分子机制上发展了哪些独特的适应策略?本文对近一个世纪以来有关鲸豚类渗透调节的研究成果进行了系统的归纳总结,并尝试从水盐来源、代谢途径、肾脏和皮肤的生理结构、水盐代谢的内分泌调节以及渗透调节的分子机制等几个方面对鲸类动物的渗透调节机制进行全面阐述,并就未来该领域的研究方向及亟需深入研究的科学问题进行了探讨。     

中国节小卷蛾属Noduliferola记述(鳞翅目:卷蛾科:新小卷蛾亚科)（英文）

记述节小卷蛾属Noduliferola 2种,其中首次记述了越南节小卷蛾N.insuetana Kuznetsov在中国的分布,重新描述了该属模式种节小卷蛾N.abstrusa Kuznetsov。提供了2种的检索表、成虫图和外生殖器图。研究标本除特别提及外均保存在南开大学昆虫标本室。