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ZHENG LianBin LI YongLan XI HuanJiu YU KeLi LU ShunHua SHI Rui WEN YouFeng BAO JingPing ZHANG XingHua LI YuLing REN Fu XU GuoChang 《中国科学:生命科学英文版》2015,58(2):215-217,1,3
<正>Dear Editor,Shortly after initiating the"Physical Anthropological Research on Han Chinese"research project,we applied uniform sampling methods as well as methods and instruments of measurement to obtain a complete set of measurements of physical anthropological indicators among Han populations across China.Among these measurements,body stature was a key indicator.Currently,there should be reliable 相似文献
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LEI P HE Y SHI W PENG J WU S ZHU H CHEN J SHEN G 《Acta biochimica et biophysica Sinica》2007,39(8):599-607
Myelosuppression is one of the major side-effects of most anticancer drugs. To achieve myeloprotection, one bicistronic vector encoding anti-apoptotic protein human WEE l (WEElHu) and proliferation-stimulating stem cell factor (SCF) was generated. In this study, we selected human umbilical cord blood CD34^+ cells as the in vitro model in an attempt to investigate whether WEEIHu, rather than conventional drug-resistant genes, can be introduced to rescue cells from the damage by chemotherapeutic agents such as cisplatin, adriamycin, mitomycin-c and 5-fluorouracil. Cell viability and cytotoxicity assay, colony-forming units in culture assay and externalization of phospholipid phosphatidylserine analysis showed that the expression of WEElHu and SCF in CD34^+ cells provided the cells with some protection. These findings suggest that the expression of WEElHu and SCF might rescue CD34^+ cells from chemotherapyinduced myelosuppression. 相似文献
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Wan-gang GU Jun-fa YUAN Ge-lin XU Li-juan LI Ni LIU Cong ZHANG Jian-hong ZHANG Zheng-li SHI 《Virologica Sinica》2007,22(1):21-25
BALB/c mice were immunized with purified White spot syndrome virus (WSSV). Six monoclonal antibody cell lines were selected by ELISA with VP28 protein expressed in E. coli. in vitro neutralization experiments showed that 4 of them could inhibit the virus infection in crayfish. Western-blot suggested that all these monoclonal antibodies were against the conformational structure of VP28. The monoclonal antibody 7B4 was labeled with colloidal gold particles and used to locate the VP28 on virus envelope by immunogold labeling. These monoclonal antibodies could be used to develop immun-ological diagnosis methods for WSSV infection. 相似文献
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SHI Zhaoxing* WANG Hengliang* HU Kun FENG Erling YAO Xiao HUANG Liuyu SU Guofu HUANG Peitang & HUANG Cuifen . Beijing Institute of Biotechnology Beijing China . College of Life Science Shandong Normal University Jinan China . College of Environmental Chemical Engineering Xi’an Jiaotong University Xi’an China Correspondence should be addressed to Huang Liuyu 《中国科学:生命科学英文版》2004,47(6):494-502
~~Screening and identification of Shigella flexneri 2a virulence-related genes induced after invasion of epithelial cells1. Jin, Q., Yuan, Z., Xu, J., Wang, Y., Shen, Y., Lu, W., Wang, J., Liu, H., Yang, J., Yang, P., Zhang, X., Zhang, J., Yang, G, Wu, H., Qu, D., Dong, J., Sun, L., Xue, Y, Zhao, A., Gao, Y., Zhu, J., Kan, B., Ding, K.. Chen, S., Cheng, H., Yao, Z., He, B., Chen, R., Ma, D., Qiang, B., Wen, Y, Hou, Y., Yu, J., Genome sequence of Shigella flexneri 2… 相似文献
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通过使用 PARP酶的抑制剂研究了降低PARP酶的活性对外源基因转染效率及整合作用的影响, 结果表明,转染的外源基因的吸收及瞬时表达不依赖于PARP酶的活性,而外源基因的整合作用与PARP酶的活性有关。
Abstract:In this study,the effect of lowering PARP activity on the transfection of cultured cells with foreigh genes was evaluated.The results indicated that PARP enzyme involved in the stable integration of foreign genes into the host genome.However,inhibition of PARP activity exhibited no effect on both the uptake into the cells and the expression of the forging genes. 相似文献
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张坚宣)史庆华)潘淑娟)张锡然)单祥年)余龙)ZHANG Jian-Xuan)SHI Qing-Hua)PAN Shu-Juan)ZHANG Xi-Ran)SHAN Xiang-Nian)YU Long) 《遗传》1999,21(1):11-15
21三体是人类最常见的先天性疾病,约95%患儿的超数21号染色体来源于母亲,且随母龄增加患儿出生率也随之增加。本文首次运用胞质分裂阻滞法(CB法)对不同年龄女性体细胞进行荧光原位杂交,对其21号染色体的分离情况进行分析。结果表明,随年龄的增加,女性体细胞中21号染色体不分离也随之增加,但21号染色体丢失无年龄效应, 且21号染色体不分离频率远高于其丢失。该结果表明,女性体细胞中21号染色体不分离与生殖细胞同样存在年龄效应。
Abstract Trisomy 21 is the most common genetic desease in human. More than 90% is derived from mother. With the advancing of mother's age, the frequency of trisomy 21 is increasing. We detected the relation between chromosome 21 missegregation and age in cytokinesis-blocked female lymphocytes by in situ hybridization with chromosome 21 specfic probe. We have found that the age effect also exists in female somatic cells as in gamets. 相似文献
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长江流域水牛血液蛋白多态性研究 总被引:12,自引:1,他引:11
史荣仙 付茂忠 赖松家 左福元 董学虎 高蓉 吴东江 陈润文 李克勇 赵廷述 余超群 黄铁夫 蒋长宇SHI Rong-Xian FU Mao-Zhong LAI Song-Jia ZUO Fu-Yuan DONG Xue-Hu GAO Rong WU Dong-Jiang CHEN Run-Wen LI Ke-Yong ZHAO Ting-Shu YU Chao-Qun HUANG Ti-Fu JIANG Chang-Yu 《遗传》1995,17(1):7-11
采集长江流域德昌、涪陵、江汉、滨湖和海子5个水牛地方品种共416 头牛的血样。电泳检测血红蛋白、血清运铁蛋白多态性,估算各等位基因频率及基因型频率,根据Hb及Tf两个位点的基因频率进行聚类分析。结果表明,5个水牛地方品种间遗传变异较小,其亲缘关系较近。 相似文献
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通用线性模型筛选家禽生化多态遗传标记的探讨 总被引:8,自引:2,他引:6
吴晓林 施启顺 柳小春 沈乃民 李胜 程光潮 刘坤凡WU Xiao-Lin SHI Qi-Shun LIU Xiao-ChunSHENG Nai-Min LI Sheng CHENG Guang-Chao LIU Kun-Fan 《遗传》1997,19(2):27-30
本研究首次采用线性模型估计生化基因(表)型对产蛋量相关性状的效应,排除环境和遗传背景的差异,检测出效应显著的3种生化基因(表)型。Akp座位F型和Es-1座位AB型的开产蛋重显著降低, 效应的遗传本质有待于深入研究确认。 Tf位点对于36周龄体重的影响不属于基因多效或紧密连锁效应, 可能是距离较远基因连锁的结果,效应随基因间连锁与交换会在品系和世代间产生较大波动;或可能源于非控制误差。本研究还表明生化基因座位与数量性状基因位点(QTL)间的非紧密基因连锁可能大量存在, 但育种意义不大。
Abstract:The literature on associations between biochemical polymorphisms with a Mendelian basis and quantitative traitsis abundant,but it is often difficult to draw conclusions from results that vary in statistical significance and that are sometimes conflicting.Linear models were found capable in estimating associated effects of biochemical genes,precluding deviations due to environmental and population differences.Three biochemical gene- or phenotypes were detected to be significantly associated with laying-related traits.F of Akp locus and AB of Es-1 locus showed large associated effects on egg weight at onset of lay as the result of pleiotrophy or close linkage,or caused by heterogeneity,whereas association of BB at Tf locus with body weight at 36 weeks of age could be due to loose linkage or non-controlled errors which exhibited large variations between populations and possibly through generations Many biochemical loci were also revealed to be occasionally associated with laying-related traits,but few of them could be of breeding importance. 相似文献