129S2/SvPasCrl小鼠肠道在衰老过程中的变化

Dear Editor:We would like to show"changes in intestinal lengths of 129 S2/SvPasCrl mice from adulthood to agedness".The intestine functions not only in digestion,absorption and excretion,but also in the regulation of central nervous system through its microbiota so as called gut-brain axis[1].As a tubular organ,the intestinal length is regarded as a significant morphological characteristic,which directly affects digestion and absorption[2].Thus,clarifying changes in intestinal lengths at different ages is beneficial to understand the functions of the digestive system as well as the aging process.     

研究报告：蚓激酶同工酶降解乙型肝炎抗原并保护肝功能

目的蚓激酶同工酶(LKIs)作为肠溶胶囊的有效成分,用于治疗血栓性疾病已有30多年历史。近年来,LKIs在其他危重疾病中的研究时有报道。本文关注LKIs在乙型肝炎方面的作用。方法乙型肝炎表面抗原(HBs Ag)、核心抗原(HBcAg)和e抗原(HBeAg)分别与不同浓度LKIs孵育,观察这些蛋白质的降解和估计肽链的切割位点。Hep G2.2.15细胞与LKIs孵育,采用酶联免疫吸附测定(ELISA)和蛋白质印迹(Western blotting)检测细胞分泌的HBsAg和HbeAg。LKIs灌胃Balb/c小鼠30天,采用ELISA和Western blotting检测其血清HBsAg和HBeAg,免疫组化染色检测肝组织中的HBcAg。采用苏木精-伊红染色分析乙肝病毒转基因小鼠肝组织的损害,并通过ELISA定量分析血清谷草转氨酶(GOT)和谷丙转氨酶(GPT)。腹腔注射后,取大鼠血清和肝组织,测定其中的LKIs含量,从而观察LKIs的吸收。采用LKIs给龙岩麻鸭灌胃30天,通过PCR检测其血清HBV DNA。结果蚓激酶肠溶胶囊的有效成分是含有6种LKIs的复方蛋白酶药物,可以降解HBV...     

Letter to Editor: 雌性GK糖尿病大鼠的核糖，AGEs和转酮酶变化

<正>Dear Editor,Type 2 diabetes is a chronic disease characterized by high glucose levels in the blood. Recently, it has been found that type 2 diabetic patients suffer from glucose and ribose dysmetabolism[1-3]. The GotoKakizaki (GK) rat is a nonobese, nonhypertensive model of type 2 diabetes, which, like humans, shares a susceptibility locus on chromosome 10[4-5].