9. The algal flora of the Primorsky Region, Russian Far East

A survey of the freshwater algal flora of the Primorsky Region, Russian Far East, is presented. During more than 70 years of investigations 1659 species (including varieties and forms 2276 taxa) belonging to 346 genera of different groups of algae have been recorded from this territory. The Primorsky Region is rich in habitats attractive to algae-rivers, lakes, reservoirs, and ponds. The details of the distribution of algae in different types of waterbodies have been examined. The algal flora of the region is comparable to the areas, and its taxonomical structure is typical for Eurasia. From this territory some new species and varieties have been described — Achnanthes chankensis, Cymbella ventricosa var. hankensis, Surirella alisoviana, and others. The flora includes some rare species — Fragilaria magocsyi, Perone dimorpha, and Heterothrix monochloron, and some endemics of Asia — Navicula scutelloides var. baicalensis, N. globulifera var. nipponica, Gomphonema sphaerophorum, and Surirella tientsinensis.     

A salt on the bioenergy and biological invasions debate: salinity tolerance of the invasive biomass feedstock Arundo donax

Arundo donax L., commonly known as giant reed, is promising biomass feedstock that is also a notorious invasive plant in freshwater ecosystems around the world. Heretofore, the salt tolerance of A. donax had not been quantified even though anecdotal evidence suggests halophytic qualities. To test whole-plant and leaf level responses, we established a pot experiment on 80 scions propagated from an A. donax population that has naturalized on the shore of the San Francisco Bay Estuary. To quantify growth and physiological responses to salinity (NaCl), A. donax scions were divided into eight treatments and grown for 60 days across a range of salinities (0–42 dS m⁻¹). Classic growth analysis showed >80% reduction in overall growth at the highest salinities. Yet, there was zero mortality indicating that A. donax is able to tolerate high levels of salt. Declining photosynthesis rates were strongly correlated (R² > 0.97) with decreasing stomatal conductance, which was in turn closely related to increasing salinity. Leaf gas exchange revealed that stomata and leaf limitations of carbon dioxide were three times greater at high salinities. Nonetheless, even when salinities were 38–42 dS m⁻¹ A. donax was able to maintain assimilation rates 7–12 μmol m⁻² s⁻¹. Further, by maintaining 50% relative growth at salinities ~12 dS m⁻¹ A. donax can now be classified as ‘moderately salt tolerant’. A. donax leaf gas exchange and whole-plant salt tolerance are greater than many important food crops (i.e. maize, rice), the bioenergy feedstock Miscanthus × giganteus, as well as some uncultivated plant species (i.e. Populus and Salix) that are indigenous in regions A. donax currently invades. The results of this study have implications for both agronomists wishing to expand A. donax to fields dominated by saline soils, and for others who are concerned about the spread of A. donax with altered stream hydrology or sea-level rise.     

What Are the Health Risks of Eating Red Meat,and How Should We Assess Them?

This paper discusses possible mechanisms that might lead to misinterpretations of collected data and makes new evidence-based medicine (EBM) recommendations to oppose the previously accepted preventive measures, or treatment options. It is focused on the danger of the “red meat” consumption, and the question whether eating pungent food is good or bad for our health and finally whether the “bad luck” concept of getting several cancer types is valid or not. These three topics got and still have significant media attention. Several mechanisms are proposed as possible causes of these apparent conflicts. Some of them have already been recognized but sadly remained less known to medical readers and also to the general population. Also see the video abstract here https://youtu.be/owjoRXrNShA .     

Erythrogenic toxins A, B and C: Occurrence of the genes and exotoxin formation from clinical Streptococcus pyogenes strains associated with streptococcal toxic shock-like syndrome

We report the study of 53 clinical isolates of group A streptococci, all from patients with streptococcal toxic shock-like syndrome. The strains were analysed for the occurrence of the genes of erythrogenic toxins (pyrogenic exotoxins) types A, B and C and in vitro production of these toxins. In contrast to reports indicating that 85% of the toxic shock-like syndrome-associated isolates contained the erythrogenic toxin A gene, only 58.5% of our strains harboured this gene. The erythrogenic toxin C gene was detected in 22.6% of the isolates. Erythrogenic toxin A and erythrogenic toxin B were produced by 68.7% and 58.3% of the strains containing either gene. For all group A streptococci, irrespective of clinical association, the erythrogenic toxin B gene was detected in all the isolates tested. Thus, it is difficult to define a specific role for erythrogenic toxin B in toxic shock-like syndrome as there was no clear correlation between this disease and the presence of toxin genes. Our results suggest the existence of other pathogenic factor(s) produced by group A streptococci which may stimulate human peripheral T lymphocytes in a manner similar to that of erythrogenic toxins, thus explaining different observations in previous epidemiological genetic studies.     

A three-step screening procedure to identify the mode of phloem loading in intact leaves

A three-step screening method was developed to identify the mode of phloem loading in intact leaves. Phloem loading of ¹⁴CO₂-derived photosynthate was challenged by p-chloromercuribenzenesulfonic acid (PCMBS) in leaves of dicotyledons with either a symplasmic (type 1, with intermediary cells as companion cells) or apoplasmic (type 2b, with transfer cells as companion cells) minor-vein configuration. Firstly, photosynthate export as the result of phloem loading was measured by collection of phloem exudate from the petiole. The PCMBS had virtually no effect on photosynthate export in representatives of type-1 families (Lamiaceae, Lythraceae, Onagraceae, Saxifragaceae). In contrast, photosynthate export was strongly reduced by PCMBS in representatives of type-2b families (Asteraceae, Balsaminaceae, Dipsacaceae, Linaceae, Tropaeolaceae, Valerianaceae) and type-2b members of polytypical families (Fabaceae, Scrophulariaceae). Secondly, densitometric measurements of leaf autoradiographs demonstrated that the contrast between the mesophyll and the lower-order veins was hardly affected by PCMBS treatment in type-1 species, whereas PCMBS strongly reduced the contrast in type-2b species. Thirdly, separate ¹⁴C-radioassays of vein and mesophyll tissues confirmed this observation. The three-step procedure thus revealed a strong and consistent reduction of phloem loading by PCMBS in type-2b species which was absent in type-1 species. In conclusion, phloem loading in type-2b species occurs via the apoplast and type-1 species execute an alternative — most likely symplasmic — mode of phloem loading.Abbreviations PCMBS p-chloromercuribenzenesulfonic acid - SE/CC-complex sieve element/companion cell complex We gratefully acknowledge the expert help of Dr. Maarten Terlou, Department of Image Processing and Design, University of Utrecht, in carrying out the densitometric measurements.     

Evaluation of past and potential phosphorus uptake at the Orlando Easterly Wetland

The Orlando Easterly Wetland (OEW), located near Christmas, Florida, USA, is among the longer-lived treatment wetlands in the United States. It was established in the late 1980s to reduce nitrogen and phosphorus concentrations from tertiary treated wastewater bound for the St. Johns River. A goal of 0.07 mg/l total phosphorus concentration has been set by the regulating agency (St. Johns River Water Management District). In order to understand and define the operating conditions for which this target could be met, a systematic study of historic phosphorus uptake was performed using a traditional first-order model. Phosphorus uptake performance is shown to correlate well with hydraulic performance for two parallel upstream cells. The first-order model is enhanced with predictive capabilities that acknowledge the correlation between the phosphorus uptake rate constant and the hydraulic loading rate observed in the system. Inherent limitations with the first-order modeling approach are addressed and uncertainty in model performance is used to bound predictions.     

Effects of training on axial muscle of two cyprinid species: Chondrostoma nasus (L.) and Leuciscus cephalus (L.)

The effect of an endurance training program lasting 17 weeks was studied in two cyprinid species, Chondrostoma nasus (L.) and Leuciscus cephalus (L.). Red, intermediate and white axial muscle were investigated. Morphometrical analysis revealed that training induced, in both species, increased red and intermediate muscle mass, fibre diameter and capillarization. Differences between species in the response to training were observed for volume densities of mitochondria and lipid. In contrast to C. nasus, L. cephalus show higher values for these compartments in red and intermediate fibres. The results are considered adaptational changes which increase the aerobic capacity of red and intermediate muscle fibres to meet higher sustained swimming activities.     

Pollen-based biome reconstruction for southern Europe and Africa 18,000 yr bp

Pollen data from 18,000 ¹⁴C yr bp were compiled in order to reconstruct biome distributions at the last glacial maximum in southern Europe and Africa. Biome reconstructions were made using the objective biomization method applied to pollen counts using a complete list of dryland taxa wherever possible. Consistent and major differences from present‐day biomes are shown. Forest and xerophytic woods/scrub were replaced by steppe, both in the Mediterranean region and in southern Africa, except in south‐western Cape Province where fynbos (xerophytic scrub) persisted. Sites in the tropical highlands, characterized today by evergreen forest, were dominated by steppe and/or xerophytic vegetation (cf. today’s Ericaceous belt and Afroalpine grassland) at the last glacial maximum. Available data from the tropical lowlands are sparse but suggest that the modern tropical rain forest was largely replaced by tropical seasonal forest while the modern seasonal or dry forests were encroached on by savanna or steppe. Montane forest elements descended to lower elevations than today.     

Distribution of plant functional types along gradients of disturbance intensity and resource supply in an agricultural landscape

Abstract. In this study, plant functional types are understood as groups of plants with similar biological traits displaying significant optima or maxima on a gradient plane of resource supply and disturbance intensity. The biological traits refer to expansion, vegetative regeneration, generative reproduction, dispersal and seed bank longevity. 129 vegetation samples were taken in an agricultural landscape in southwestern Germany, covering a wide range of terrestrial vegetation types – but with the exception of forests and wetlands. For each site, also soil data were recorded. Mean daily soil moisture was estimated with a simple model. Soil moisture, balanced nitrogen supply and available phosphorus were combined into a factor ‘resource supply’. In addition, disturbance intensity was estimated for each site. This factor was based on (1) frequency of disturbance, (2) disturbance depth below or above the soil surface, and (3) proportion of the area affected by a discrete disturbance event. 30 plant groups with similar biological characteristics resulted from a cluster analysis, based on a compilation of 19 biological traits for a regional species pool. Logistic regression on a gradient plane of disturbance intensity and resource supply yielded response curves for 28 groups. The dependent variable was defined as the probability of encountering all members of a group in a sample. 17 groups display a significant response curve on the gradient plane. Plants with a potential for long- range dispersal are concentrated on sites with low or high disturbance intensities (e.g. fallow land, fields, lawns). On sites with medium disturbance intensity (e.g. meadows) and low to medium resource supply, small-range dispersal predominates. There are no distinct trends concerning seed bank longevity. The potential for vertical and lateral expansion increases with decreasing disturbance intensity. Only at medium disturbance intensities does vertical expansion correlate positively with resource supply. Rapid detachment of daughter individuals occurs more often on productive sites than on less productive sites. Diversity of groups with similar biological traits is highest on sites with medium disturbance intensities.