全文获取类型
收费全文 | 19358篇 |
免费 | 1961篇 |
国内免费 | 6502篇 |
出版年
2023年 | 551篇 |
2022年 | 617篇 |
2021年 | 822篇 |
2020年 | 972篇 |
2019年 | 1142篇 |
2018年 | 1046篇 |
2017年 | 974篇 |
2016年 | 982篇 |
2015年 | 1023篇 |
2014年 | 1167篇 |
2013年 | 1716篇 |
2012年 | 1030篇 |
2011年 | 1088篇 |
2010年 | 868篇 |
2009年 | 1129篇 |
2008年 | 1040篇 |
2007年 | 1149篇 |
2006年 | 1059篇 |
2005年 | 927篇 |
2004年 | 835篇 |
2003年 | 813篇 |
2002年 | 692篇 |
2001年 | 564篇 |
2000年 | 513篇 |
1999年 | 466篇 |
1998年 | 402篇 |
1997年 | 369篇 |
1996年 | 342篇 |
1995年 | 356篇 |
1994年 | 363篇 |
1993年 | 312篇 |
1992年 | 274篇 |
1991年 | 225篇 |
1990年 | 212篇 |
1989年 | 231篇 |
1988年 | 169篇 |
1987年 | 141篇 |
1986年 | 121篇 |
1985年 | 157篇 |
1984年 | 191篇 |
1983年 | 115篇 |
1982年 | 162篇 |
1981年 | 93篇 |
1980年 | 85篇 |
1979年 | 85篇 |
1978年 | 48篇 |
1977年 | 29篇 |
1976年 | 39篇 |
1974年 | 30篇 |
1973年 | 27篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
A. Rigotto S.R. Cotta A.C.F. Dias J.L.N. Carvalho F.D. Andreote 《Letters in applied microbiology》2020,71(5):444-450
Sugarcane cultivation supports Brazil as one of the largest world sugar and ethanol producer. In order to understand the impact of changing sugarcane harvest from manual to mechanized harvest, we studied the effect of machinery traffic on soil and consequently soil compaction upon soil microbial communities involved in nitrogen cycling. The impact of sugarcane harvest was dependent on soil depth and texture. At deeper soil layers, mechanized harvesting increases the abundance of nitrogen fixers and denitrifying communities (specifically nosZ clade I and II) while manual harvesting increases the abundance of ammonia oxidizers (specifically AOA) and increases denitrifying communities (nosZ clade I and II) on top and at intermediate depth. The effect of change on the harvest system is more evident on sandy soil than on clay soil, where soil indicators of compaction (bulk density and penetration resistance) were negatively correlated with soil microorganisms associated with the nitrogen cycle. Our results point to connections between soil compaction and N transformations in sugarcane fields, besides naming biological variables to be used as proxies for alterations in soil structure. 相似文献
2.
Interleukin-1βconverting enzyme: A novel cysteine protease required for IL-1β production and implicated in programmed cell death 下载免费PDF全文
Nancy A. Thornberry Susan M. Molineaux 《Protein science : a publication of the Protein Society》1995,4(1):3-12
Interleukin-1β converting enzyme is the first member of a new class of cysteine proteases. The most distinguishing feature of this family is a nearly absolute specificity for cleavage at aspartic acid. This enzyme has been the subject of intense research because of its role in the production of IL-1β, a key mediator of inflammation. These studies have culminated in the design of potent inhibitors and determination of its crystal structure. The structure secures the relationship of the enzyme to CED-3, the product of a gene required for programmed cell death in Caenorhabditis elegans, suggesting that members of this family function in cell death in vertebrates. 相似文献
3.
Samaneh Shojaei Seyed Mahmoud Hashemi Hossein Ghanbarian Mohammad Salehi Samira Mohammadi-Yeganeh 《Journal of cellular physiology》2019,234(4):3394-3409
Mesenchymal stem cells (MSCs) are multipotent cells with the potential to differentiate into different cell types. Owing to their immunosuppressive and anti-inflammatory properties, they are widely used in regenerative medicine, but they have a dual effect on cancer progression and exert both growth-stimulatory or -inhibitory effects on different cancer types. It has been proposed that these controversial effects of MSC in tumor microenvironment (TME) are mediated by their polarization to proinflammatory or anti-inflammatory phenotype. In addition, they can polarize the immune system cells that in turn influence tumor progression. One of the mechanisms involved in the TME communications is extracellular vesicles (EVs). MSCs, as one of cell populations in TME, produce a large amount of EVs that can influence tumor development. Similar to MSC, MSC-EVs can exert both anti- or protumorigenic effects. In the current study, we will investigate the current knowledge related to MSC role in cancer progression with a focus on the MSC-EV content in limiting tumor growth, angiogenesis, and metastasis. We suppose MSC-EVs can be used as safe vehicles for delivering antitumor agents to TME. 相似文献
4.
Protected cultivation of ornamental flowers, as a commercial venture, becomes less profitable with excessive use of fertilizers. The present study examined the influence of microbial biofilm inoculants (Anabaena–Azotobacter, Anabaena–Trichoderma and Trichoderma–Azotobacter) on the availability of soil nutrients and structure of rhizosphere microbial communities in three varieties of chrysanthemum (var. White Star, Thai Chen Queen and Zembla). Varietal-specific responses in growth, enzyme activities, flower yield of plants and availability of soil nutrients were recorded. Dehydrogenase activity was highest in var. White Star treated with the Anabaena–Trichoderma biofilm inoculants. The Anabaena–Azotobacter inoculant enhanced the availability of nitrogen, phosphorus and micronutrients in the soil, besides 40–50% increase in soil organic carbon, as compared to carrier alone or no inoculation. PCR-DGGE profiling of the cyanobacterial communities and qPCR quantification of 16S rRNA abundance of bacteria, archaea and cyanobacteria in the rhizosphere soils, revealed the stronger influences of these inoculants, especially in var. Zembla. Principal Component Analysis (PCA) helped to illustrate that the enhanced microbe-mediated availability of soil macro-and micronutrients, except iron content (Fe), was the most influential factor facilitating improved plant growth and yield parameters. The Anabaena–Azotobacter, and Anabaena–Trichoderma biofilm inoculants, proved superior in all three chrysanthemum varieties. 相似文献
5.
6.
7.
9.
《Bioorganic & medicinal chemistry》2014,22(1):141-147
A series of new sulfonamides was prepared starting from 2-oxo-N′-(4-sulfamoylphenyl)-propanehydrazonoyl chloride, a sulfanilamide derivative, which was reacted with aroylhydrazides, amines, or thiols. A library of derivatives incorporating aroylhydrazone, [1,2,4]triazolo[3,4-b][1,3,4]thiadiazinyl- or 2-(cyanophenyl-methylene)-1,3,4-thiadiazol-3(2H)-yl moieties was thus synthesized. The new compounds were investigated as inhibitors of four α-carbonic anhydrases (CAs, EC 4.2.1.1), the human (h) isoforms hCA I and II, and the bacterial ones recently isolated from the extremophilic bacteria Sulfurihydrogenibium yellostonense (SspCA) and Sulfurihydrogenibium azorense (SazCA). Low nanomolar activity was observed against hCA II (KIs of 0.56–17.1 nM) whereas hCA I was less inhibited by these compounds (KIs of 86.4 nM–32.8 μM). The bacterial CAs were also effectively inhibited by these derivatives (KIs in the range of 0.77–234 nM against SazCA, and of 6.2–89.1 against SspCA, respectively), with several low nanomolar/subnanomolar inhibitors detected against both of them. As SspCA and SazCA are among the most thermostable and catalytically active CAs, it is of interest to find modulators of their activity for potential biotechnologic applications. 相似文献
10.
Folsomia Candida was maintained on potato dextrose agar (PDA) plates precolonised by the mycoparasite Coniothyrium minitans for 3 yr but the sciarid Bradysia sp. survived for a maximum of only three generations. Collembolans and sciarid larvae from these cultures were able to transmit C. minitans to uninoculated PDA plates through the survival of spores in faecal pellets. Adult and larval sciarids also transmitted C. minitans from PDA culture to uninoculated PDA plates by contamination on the cuticle. In soil and sand both sciarids and collembolans were able to transmit C. minitans from C. m/m'tans-inoculated to uninoculated sclerotia of Sclerotinia sclerotiorum. Inoculation of sclerotia with C. minitans enabled greater populations of larger collembolans to develop. In the glasshouse where C. minitans had been applied to the soil, one adult sciarid and four collembolans out of 70 and 101 insects collected respectively yielded C. minitans on placement onto PDA + Aureomycin. 相似文献