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2.
Shuanxi Fan 《人类与生态风险评估》2017,23(5):1099-1120
Soil heavy metal pollution from mining activities is potentially harmful to human health through the food chain. In this study, a total of 43 soil samples were collected from a depth of 0–20 cm from fields close to a Pb and Zn smelter. The samples were used to: 1) analyze the pollution level of heavy metals (Pb, Zn, Cr, and Cu) and spatial distribution pattern; 2) evaluate the degree of accumulation and enrichment, potential ecological risk, and human health risk; and 3) perform source apportionment in Fengxiang County, Shaanxi Province of China. The results showed that the concentration ranged from 43.67 to 189.55, 131.43 to 239.53, 74.77 to 112.25, and 24.69 to 37.71 mg·kg?1 for Pb, Zn, Cr, and Cu, respectively, and the mean concentration for Pb, Zn, Cr, and Cu was 129.46, 192.85, 91.98 and 31.67 mg·kg?1, respectively. The concentrations were greater than the Shaanxi Province background values, while they were lower than the second-level limits of Environmental Quality Standard for Soils of China (EQSS). The spatial distribution of heavy metal contents showed a banded in soil except Cu. The spatial distribution pattern and pollution assessment indexes (Igeo, EF) indicated that the investigated metals had been accumulated in the study areas, and implied significant influences from anthropogenic activities, local meteorological situation, and soil properties. The ecological risk assessment showed that the risks were relatively low (RI<150). Compared with the exposure risk for adults, that for children was significantly greater. The ingestion of heavy metals in the soils by humans was the main exposure pathway compared with the dermal exposure. There may be a risk of noncarcinogenic adverse health effects (HQ < 1, 0.377 ≤ HI≤1.553) on children, but the adults were unlikely to experience obvious adverse health effects (HQ < 1, HI < 1). The carcinogenic risk of Cr for adults and children was at an unacceptable level. The carcinogenic and noncarcinogenic risks were in the order of children > adults. The correlation analysis showed that Pb, Cr, and Cu have identical anthropogenic and natural sources, while Zn has another identical source. This study could provide a basis for the sustainable management of this region by reducing metal inputs and to protect soils from long-term heavy metal accumulation. 相似文献
3.
Conidiobolus thromboides is an entomophthoralean fungus with potential as a biological control agent of aphids. However, its application in biological control is limited due to its formulation requirements. The objective of this study was to develop and optimise a novel air-extrusion method to embed C. thromboides hyphae at high density in alginate pellets. An orthogonal experimental design was used to investigate selected combinations of parameters known to affect hyphal density within pellets. The diameter of pellets produced, and the calculated density of hyphae within them, ranged from 0.18 ± 0.09 to 3.17 ± 0.06 mm and from 0.02 to 350.56 mg/mm3 respectively. These data were used to predict the optimal parameter combination to deliver the greatest density of hyphae of C. thromboides per pellet: 1% sodium alginate, a 1:2 ratio of hyphae to sodium alginate, an orifice diameter of 0.232 mm and an air pressure of 0.05 MPa. Pellets made under the optimal conditions predicted produced a mean total of 4.3 ± 0.6 × 105 conidia per pellet at 100% relative humidity which was significantly greater than the mean total number of conidia produced from infected aphid cadavers of comparable size (9.35 ± 0.85 × 104) (p < 0.001). In conclusion, air-extrusion embedding appears to be a promising method for formulating in vitro-produced hyphae of C. thromboides for use in biological control. 相似文献
4.
R. E. Hoagland 《Biocontrol Science and Technology》1995,5(3):251-260
A rapid bioassay was developed to measure the bioherbicidal efficacy of spore preparations of the pathogens Colletotrichum truncatum (Schwein.) Andrus and W. D. Moore and Alternaria cassiae Jurair and Khan on hemp sesbania (Sesbania exaltata) and sicklepod (Cassia obtusifolia), respectively. The system uses 4-day-old dark-grown seedlings (grown hydroponically in paper towel cylinders) which were sprayed with spore suspensions. Shoot lengths were monitored non-destructively, and recorded over time under conditions of dark growth, 90-100% relative humidity and 25 C. Shoot growth inhibition and stem collapse (mortality) were directly related to the spore concentration applied. Generally, at 10 3 - 10 4 spores ml-1, these pathogens caused significant shoot growth inhibition within 25-30 h and seedling death within 40-50 h. This bioassay has been used to study herbicide-pathogen interactions, and may be extended to determine the bioherbicidal efficacy of different pathogen isolates, pathovars or spore formulations. This technique is more rapid, uses a lower inoculum volume, requires less space and is performed under more controlled conditions than conventional greenhouse bioassay methods. The data obtained are more quantitative than those obtained from bioassays relying on visual rating systems. 相似文献
5.
《Bioorganic & medicinal chemistry letters》2020,30(14):127117
A small set of indole-2-carboxamide derivatives identified from a high-throughput screening campaign has been described as a novel, potent, and glucose-sensitive inhibitors of glycogen phosphorylase a (GPa). Among this series of compounds, compound 2 exhibited moderate GP inhibitory activity (IC50 = 0.29 μM), good cellular efficacy (IC50 = 3.24 μM for HepG2 cells and IC50 = 7.15 μM for isolated rat hepatocytes), together with good absorption, distribution, metabolism, and elimination (ADME) profiles. The in vivo animal study revealed that compound 2 significantly inhibited an increase of fasting blood glucose level in adrenaline-induced diabetic mice. 相似文献
6.
Molly T. Townsend 《Computer methods in biomechanics and biomedical engineering》2016,19(11):1137-1142
Simplified material models are commonly used in computational simulation of biological soft tissue as an approximation of the complicated material response and to minimize computational resources. However, the simulation of complex loadings, such as long-duration tissue swelling, necessitates complex models that are not easy to formulate. This paper strives to offer the updated Lagrangian formulation comprehensive procedure of various non-linear material models for the application of finite element analysis of biological soft tissues including a definition of the Cauchy stress and the spatial tangential stiffness. The relationships between water content, osmotic pressure, ionic concentration and the pore pressure stress of the tissue are discussed with the merits of these models and their applications. 相似文献
8.
《Animal : an international journal of animal bioscience》2015,9(5):888-898
Free-range birds such as organic broilers may ingest soil and plants during exploration. The estimation of such intakes is of great interest to quantify possible nutritional supplies and also to evaluate the risk of exposure to parasites or to environmental contaminants. Marker-based techniques are now available and would allow to quantify plant and, especially, soil intake in free-range birds, and this quantification was the aim of this study. Methodologically, the proportion of plants in diet intake was determined first using a method based on n-alkanes. Subsequently, the fraction of soil in the total intake was estimated with a second marker, acid-insoluble ash. This approach was carried out to estimate ingested amounts of plants and soil for five successive flocks of organic broilers, exploring grass-covered yards or those under trees, at two time points for each yard: 51 and 64 days of age. Each factor combination (yard type×period=flock number×age) was repeated on two different yards of 750 broilers each.The birds’ plant intake varied widely, especially on grass-covered yards. The proportion of plant intake was significantly higher on grass-covered plots than under trees and was also affected, but to a lesser extent, by age or flock number. The ingestion of plants would generally not exceed 11 g of DM daily, except two extreme outliers of nearly 30 g. The daily plant intake under trees tended to be lower and never exceeded 7 g of DM. The amount of ingested plants increased significantly for spring flocks. It increased slightly but significantly with age. The proportion of ingested soil was significantly higher under trees than on grass-covered yards. Dry soil intake was generally low with not more than 3 g per day. Only in adverse conditions – that is, older birds exploring yards under trees in winter – soil intake reached the extreme value of nearly 5 g. Broilers on yards under trees ingested significantly more soil than on grass-covered yards with least square means of, respectively, 2.1 and 1.1 g dry soil per day. These quantifications would allow us to evaluate the impact of plant and soil intake in the management of free-range broilers, especially for the management in organic farming systems. Nevertheless, under the two rearing conditions tested in the current study, the quite low proportions of soil intakes would represent a low risk for the safety of the produced food, unless the birds explore yards on heavily contaminated soil. 相似文献
9.
Habitat management under the auspices of conservation biological control is a widely used approach to foster conditions that ensure a diversity of predator species can persist spatially and temporally within agricultural landscapes in order to control their prey (pest) species. However, an emerging new factor, global climate change, has the potential to disrupt existing conservation biological control programs. Climate change may alter abiotic conditions such as temperature, precipitation, humidity and wind that in turn could alter the life-cycle timing of predator and prey species and the behavioral nature and strength of their interactions. Anticipating how climate change will affect predator and prey communities represents an important research challenge. We present a conceptual framework—the habitat domain concept—that is useful for understanding contingencies in the nature of predator diversity effects on prey based on predator and prey spatial movement in their habitat. We illustrate how this framework can be used to forecast whether biological control by predators will become more effective or become disrupted due to changing climate. We discuss how changes in predator–prey interactions are contingent on the tolerances of predators and prey species to changing abiotic conditions as determined by the degree of local adaptation and phenotypic plasticity exhibited by species populations. We conclude by discussing research approaches that are needed to help adjust conservation biological control management to deal with a climate future. 相似文献
10.