小克银汉霉核糖体DNAPCR扩增产物的限制性片段长度多型性

应用一对寡苷酸引物ＩＴＳ１与ＩＴＳ４对核ＤＮＡＧ＋Ｃ百分数小于３０％的小克银汉霉属的核糖体ＤＮＡ（ｒＤＮＡ）内转录间区（ＩＴＳ）进行了扩增。测试的属于１０个种和变种的２２株菌都得到了扩增产物，在同属不同种之间扩增的ＩＴＳ片段长度有巨大差别。据此可分为三组。这三组所含分类群数及其ＤＮＡ长度 ：第一组４种１变种，小于７６４碱基对；第二组２种，７６５－８２４ｂｐ；第三组２种１变种，大于８２５ｂｐ。所研究     

细菌RNA分离试剂盒

Bioscience Technology 2003年7月31页报道：美国Amersham Biosciences公司最近推出GenomiPhi^TM DNA扩增试剂盒。利用这种试剂盒可从有限的生物材料制备高质量的DNA。这是一种简单的等温扩增技术，它不需要使用热循环器。原材料可以是来自全血、颊面拭子、细胞印迹纸、     

盘霜霉一新种—岩参盘霜霉

本文报道了采自我国新疆天山西部寄生于菊科植和兰花岩参上的盘霜霉菌新种－岩参盘霜霉 。对该种的形态作了拉丁文和汉文描述。并附有特征。图。模式标本保存于新疆农业大学真菌菌标本室。     

棘豆属三种植物体外培养再生植株

PlantletRe郡nerattonfromThreespeciesof山涉oplsInv咖0LIUMing－Zhi（a仲伽响ofeq，Imlz’xlho，LnNz～xl730000）1植物名称棘豆属：黄花棘豆（Oxytropisochrocephala）、甘肃棘豆（O.kansuensis）和宽苞棘豆（O.latirateata）。2材料类别由种子萌发的无菌苗下胚轴以及子叶的切段和切块。3培养条件使用B5和MS及改良的MS培养基[MS大量元素的CaCl2·2H2O、MgSO4；·7H2O、KH2PO4不变，改变NO3和NH浓度及比例。因此，在培养基中除加入不同比例及不同量的KNO3和NH4NO3外，还添加适量的氯化钾和柠檬酸按。No：NH分别为0：6…     

中国冠盘藻属的研究

本文基于近年冠盘藻属研究的新成果,对产于中国的七个种和变型作分类学及形态学、产地、生境、分布的记述。对冠盘藻属研究中的一些问题提出讨论意见。     

福建冬青属二新种

本文描述了发现于福建的冬青科冬青属2新种——泰宁冬青Ilex tainingensis G. S. He和南平冬青I. nanpingensis G. S. He，并分别说明两者与其他近缘种的区别要点。泰宁冬青与太平山冬青I. sugerokii Maxim.的区别在于前种小枝被毛，无棱槽，叶片宽卵圆形，花淡紫色，分核表面具细条纹，背后中部有一凹槽。南平冬青与拟榕叶冬青I. subficoidea S. Y. Hu区别在于前种果为椭圆形，较大，表面光滑无腺点，果梗长于1 cm，每分核上端有2尖角，均可以区别。     

山西省异爪蝗属二新种（直翅目：网翅蝗科）

本文记述在晋南地区采到的异爪蝗属二新种,黑条异爪蝗Euchorthippus nigiliineatus sp.nov.及曲线异爪蝗Eochorthippus sinucarinatus sp.nov.     

Fractal root system of Melica przewalskyi along different aspect in degraded grassland

Aims Fractal root system is phenotypic plasticity result of plant root architecture to respond to environmental heterogeneity, may reflect the growth strategy of plants to adapt to environmental conditions. Our objective was to explore the relationship between root fractal dimension and fractal abundance of fractal root system of Melica przewalskyi population in response to aspect variation in the northwest of China. Methods The study site was located in a degraded alpine grassland on the northern slope in Qilian Mountains, Gansu Province, China. Survey and sampling were carried out at 40 plots which were set up along four slope aspects transects with 20 m distance between adjacent plots. Handheld GPS was used to determine the elevation, longitude and latitude of each plot. ArcGIS was used to set up digital elevation model (DEM). Community traits were investigated and six individuals roots of M. przewalskyi were collected randomly at each plot. The samples were cleaned and divided into different organs, then scanning the root with the Win-RHIZO for measurements of fractal dimension and fractal abundance in laboratory, and their biomass were then measured after being dried at 80 °C in an oven. Important findings With the slope aspect turned from north to east, west, and south, the density, height and soil moisture content of the plant community displayed a pattern of initial decline, the height, density, root fractal abundance of M. przewalskyi increased and the root fractal dimension decreased. The root fractal dimension was negatively associated with the fractal abundance in all aspects, but the relationship varied along the slope aspects gradient; there was a highly significant negative correlation (p < 0.01) between the root fractal dimension and fractal abundance at north slope and south slope aspect, whereas the correlation only reached a significant level (p < 0.05) at the east slope aspect and west slope aspect; indicating that there is a trade-off between the root fractal dimension and fractal abundance. In addition, when the slope aspect changed from north to east, west and south, the standardized major axis (SMA) slope of the regression equation in the scaling relationships between root fractal dimension and fractal abundance increased (p < 0.05), indicating that the roots of M. przewalskyi at the droughty southern slope have less branch and more sparse in the same soil volume of root exploitation and utilization. Consequently, the resource allocation pattern on reasonable trade-off between root fractal dimension and fractal abundance in different slope aspect of M. przewalskyi, reflects the relationship between the income and the cost of construction of plant root architecture.