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991.
Studies on DNA markers (D4S10 and D4S43/S127) genetically linked to Huntington's disease in Japanese families 总被引:1,自引:0,他引:1
Ichiro Kanazawa Ikuko Kondo Joh-E Ikeda Teruaki Ikeda Yuichior Shizu Mitsuo Yoshida Hirotaro Narabayashi Shigetoshi Kuroda Hisayuki Tsunoda Eiji Mizuta Yoko Okuno Kiyotaka Sugawara Miho Murata Mafuyu Takahashi James F. Gusella 《Human genetics》1990,85(3):257-260
Summary This is the first full report on the genetic linkage between Japanese Huntington's disease and the DNA markers D4S10 and D4S43/S127. With use of the HindIII, BglI, and EcoRI polymorphisms detected at D4S10, and the combination of all these polymorphisms to give composite haplotypes, nine Japanese Huntington's disease families were found to be informative. Three recombinants for D4S10 were detected in these families, giving a maximum lod score of 1.662 at a of 0.10. Similarly, when we used the MspI and PvuII polymorphisms detected by D4S43/S127, five families gave informative results. No recombinant was detected in these families, giving a maximum lod score of 3.348 at a of 0.00. These results clearly support the view that the Japanese Huntington's disease gene may be identical with the Western gene, in spite of the lower prevalence rate in Japan. 相似文献
992.
993.
Effects of DNase production, plasmid size, and restriction barriers on transformation of Vibrio cholerae by electroporation and osmotic shock 总被引:14,自引:0,他引:14
Hilda Marcus Julian M. Ketley James B. Kaper Randall K. Holmes 《FEMS microbiology letters》1990,68(1-2):149-154
Attempts to transform wild type strains of V. cholerae with plasmid DNA by traditional osmotic shock methods were not successful. A mutant of V. cholerae that was deficient in extracellular DNase was transformed with plasmid DNA by osmotic shock, demonstrating directly that extracellular DNase is a major barrier to transformation of V. cholerae. Transformation of wild type and DNase-negative strains of V. cholerae was accomplished by electroporation. Efficiency of transformation by electroporation increased with field strength, decreased with plasmid size, and was relatively insensitive to changes in the electrolyte composition of the buffer as long as isotonic sucrose was present. Host-controlled modification/restriction systems also affected transformation efficiency in V. cholerae. 相似文献
994.
Discrimination of jittered sonar echoes by the echolocating bat,Eptesicus fuscus: The shape of target images in echolocation 总被引:2,自引:0,他引:2
James A. Simmons Michael Ferragamo Cynthia F. Moss Scott B. Stevenson Richard A. Altes 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1990,167(5):589-616
1. Behavioral experiments with jittering echoes examined acoustic images of sonar targets in the echolocating bat, Eptesicus fuscus, along the echo delay or target range axis. Echo phase, amplitude, bandwidth, and signal-to-noise ratio were manipulated to assess the underlying auditory processes for image formation. 2. Fine delay acuity is about 10 ns. Calibration and control procedures indicate that this represents temporal acuity rather than spectral discrimination. Jitter discrimination curves change in phase when the phase of one jittering echo is shifted by 180 degrees relative to the other, showing that echo phase is involved in delay estimation. At an echo detectability index of about 36 dB, fine acuity is 40 ns, which is approximately as predicted for the delay accuracy of an ideal receiver. 3. Compound performance curves for 0 degrees and 180 degrees phase conditions match the crosscorrelation function of the echoes. The locations of both 0 degrees and 180 degrees phase peaks in the performance curves shift along the time axis by an amount that matches neural amplitude-latency trading in Eptesicus, confirming a temporal basis for jitter discrimination. 相似文献
995.
Characterization of lignin peroxidase-encoding genes from lignin-degrading basidiomycetes 总被引:7,自引:0,他引:7
Two closely linked lignin peroxidase (LPO)-encoding genes (lpo) from Phanerochaete chrysosporium were isolated. Nucleotide sequence studies indicated that the two genes are separated by 1.3 kb of flanking DNA and transcribed in opposite directions. Cloned P. chrysosporium lpo gene probes have been shown to hybridize to multiple sequences present in the DNAs of the white-rot fungi, Bjerkandera adusta, Coriolus versicolor and Fomes lignosus, but no hybridization was detected with DNA from Pleurotus ostreatus. Thus, lpo gene families appear to be common in a number of lignin-degrading basidiomycetes, some of which have not yet been shown to produce LPO proteins. 相似文献
996.
Structure of a mouse histone-encoding gene cluster 总被引:5,自引:0,他引:5
997.
Paul A. Faure James H. Fullard Robert M. R. Barclay 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1990,166(6):843-849
1. | Most studies examining interactions between insectivorous bats and tympanate prey use the echolocation calls of aerially-feeding bats in their analyses. We examined the auditory responses of noctuid (Eurois astricta) and notodontid (Pheosia rimosa) moth to the echolocation call characteristics of a gleaning insectivorous bat, Myotis evotis. |
2. | While gleaning, M. Evotis used short duration (mean ± SD = 0.66 ± 0.28 ms, Table 2), high frequency, FM calls (FM sweep = 80 – 37 kHz) of relatively low intensity (77.3 + 2.9, –4.2 dB SPL). Call peak frequency was 52.2 kHz with most of the energy above 50 kHz (Fig. 1). |
3. | Echolocation was not required for prey detection or capture as calls were emitted during only 50% of hovers and 59% of attacks. When echolocation was used, bats ceased calling 324.7 (±200.4) ms before attacking (Fig. 2), probably using prey-generated sounds to locate fluttering moths. Mean call repetition rate during gleaning attacks was 21.7 (±15.5) calls/s and feeding buzzes were never recorded. |
4. | Eurois astricta and P. rimosa are typical of most tympanate moths having ears with BFs between 20 and 40 kHz (Fig. 3); apparently tuned to the echolocation calls of aerially-feeding bats. The ears of both species respond poorly to the high frequency, short duration, faint stimuli representing the echolocation calls of gleaning M. evotis (Figs. 4–6). |
5. | Our results demonstrate that tympanate moths, and potentially other nocturnal insects, are unable to detect the echolocation calls typical of gleaning bats and thus are particularly susceptible to predation. |
998.
Exposure of the W3110 strain ofEscherichia coli K12 to low concentrations of glutaraldehyde or formaldehyde results in an unusual pattern of protein expression, as determined by high-resolution, two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). A decline in total protein synthesis is accompanied by the upregulation of three proteins of approximate molecular weight 38 kDa. In the presence of 0.1 mM glutaraldehyde this response occurs within the first 5 min of incubation, and with 0.1 mM formaldehyde, within the first 30 min of incubation. The 38 kDa proteins continue to be expressed at high levels until cell death. Comparison of our 2-D PAGE patterns withE. coli gene-protein and plasmid indexes indicates that one of the proteins may be the major gene product of thepyrC locus. This pattern of protein synthesis may indicate a novelE. coli stress response. 相似文献
999.
L S Bukbulatova A B Obidin N V Guliaeva M G A?rapetiants R I Kruglikov 《Biulleten' eksperimental'no? biologii i meditsiny》1990,109(4):315-316
The active avoidance training of rats resulted in a depletion of lipid peroxidation (LPO) products in cerebral cortex. LPO inhibition was also shown in cerebral cortex of "active control" group receiving +non-combined stimuli (the effect of short-term stress). LPO inhibition was more pronounced in rats staining a training criterion compared to rats which received combined stimuli but did not reach the criterion. In the active control group LPO inhibition was accompanied by total phospholipids accumulation and cholesterol depletion in cortical lipid extracts. Irrespective of attaining the criterion in all rats trained for active avoidance the accumulation of cholesterol was seen. Active avoidance training affected also the phospholipid composition of cerebral cortex. 相似文献
1000.
In vivo genetic engineering: homologous recombination as a tool for plasmid construction 总被引:3,自引:0,他引:3
This paper describes a novel method for creating exact DNA fusions between any two points in a plasmid carried in Bacillus subtilis. It exploits the homologous in vivo recombination between directly repeated sequences that can be established by insertion of a synthetic oligodeoxyribonucleotide. The method was used to enhance the productivity in B. subtilis of a cloned alpha-amylase (Amy)-encoding gene originating from Bacillus stearothermophilus. Thus, an exact fusion between nucleotide sequences encoding the expression signals, including the signal peptide, of a Bacillus licheniformis Amy-encoding gene and the mature Amy of B. stearothermophilus, was created. The resulting hybrid translational product was processed correctly in B. subtilis during secretion, giving rise to an Amy identical to the mature Amy secreted by B. stearothermophilus. 相似文献