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991.
Jing Shi Junyao Duan Huijie Gong Yuewen Pang Ling Wang Yongji Yan 《Journal of cellular and molecular medicine》2019,23(11):7268-7278
Hyperoxaluria‐induced calcium oxalate (CaOx) deposition is the key factor in kidney stone formation, for which adipose‐derived stromal cells (ADSCs) have been used as a therapeutic treatment. Studies revealed that miR‐20b‐3p is down‐regulated in hypercalciuric stone‐forming rat kidney. To investigate whether ADSC‐derived miR‐20b‐3p‐enriched exosomes protect against kidney stones, an ethylene glycol (EG)‐induced hyperoxaluria rat model and an in vitro model of oxalate‐induced NRK‐52E cells were established to explore the protective mechanism of miR‐20b‐3p. The results showed that miR‐20b‐3p levels were decreased following hyperoxaluria in the urine of patients and in kidney tissues from animal models. Furthermore, treatment with miR‐20b‐3p‐enriched exosomes from ADSCs protected EG‐induced hyperoxaluria rats, and cell experiments confirmed that co‐culture with miR‐20b‐3p‐enriched exosomes alleviated oxalate‐induced cell autophagy and the inflammatory response by inhibiting ATG7 and TLR4. In conclusion, ADSC‐derived miR‐20b‐3p‐enriched exosomes protected against kidney stones by suppressing autophagy and inflammatory responses. 相似文献
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Jeong-Won Bae So-Hye Kim Dae-Hyun Kim Jae Jung Ha Jun Koo Yi Seongsoo Hwang Buom-Yong Ryu Myung-Geol Pang Woo-Sung Kwon 《Reproductive biology》2019,19(4):356-362
Ras-related protein Rab (Rab) proteins, member of Ras superfamily of monomeric G proteins, are well known key regulators of intracellular vesicular transport. Recently, it has been reported that Rab 2A and 3A are related to acrosomal exocytosis in spermatozoa and Rab 2A can be used to fertility-related biomarker in male. However, the role and mechanism of Rab proteins in spermatozoa has not been fully understood yet. Therefore, the study to analyze the expression and location of various Rab proteins in spermatozoa is required to understand the role and mechanism of Rab proteins in spermatozoa. In present study, to analyze the expression level and location of various Rab proteins (Rab 2A, Rab3A, Rab4, Rab5, Rab8A, Rab9, Rab11, Rab14, Rab25, Rab27A, and Rab34) and Rab protein regulators (RabGAP, RabGDI, RabGEF) in spermatozoa following capacitation, immunofluorescence and western blot analysis were performed. All of 11 Rab proteins were expressed in acrosomal region and tail of spermatozoa. Furthermore, all Rab proteins and Rab protein regulators, except RabGAP, have decreased expression patterns after capacitation. Taken together, Rab proteins were located in sperm head and tail. In addition, expression patterns of Rab proteins in spermatozoa were altered following capacitation. Therefore, our results suggested that Rab proteins may be key proteins related with capacitation as well as playing important role with uniquely activation pathway for male fertility. 相似文献
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Feng Juanjuan Rao Mingjin Wang Man Liang Lin Chen Zhi Pang Xiufeng Lu Weiqiang Sun Zhenliang 《中国科学:生命科学英文版》2019,62(10):1409-1412
正Dear Editor,Pancreatic cancer (PCa) is a high-grade gastrointestinal malignancy more commonly occurring in elderly populations with lower than 5% overall 5-year survival rate (Hidalgo, 2010). PCa responds poorly to most chemotherapeutic agents, and therefore it is imperative to develop novel therapeutic agents that have anti-cancer activities against PCa. 相似文献
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Optimization and performance validation for the fully automatic blood type analysis system 下载免费PDF全文
Rongrong Pang Libo Zhang Yong Wang Wenping Han Min Huang Qiang Fu 《Blood and Genomics》2019,3(1):67-72
To evaluate and validate the application of fully automatic blood type analysis system parameters under actual lab conditions. All key system parameters were optimized and validated accordingly. The optimized parameters were centrifugal speed at 550 rpm; centrifugal time 20 min; resuspension speed 1,200 rpm; resuspension time: 45 s; incubation temperature 25℃ ; incubation time 400 s; incubation rate: 0 rpm. The sampled red blood cell concentration was 3%. The ratio of plasma to red blood cells reagent was 60 μL:30 μL; the ratio of antibody (reagent) to sampled diluted red blood cell was 30 μL:30 μL. After applying our key parameters for optimization and validation, the automatic blood type detection system's performance was found to meet the relevant requirements, effectively improving the accuracy and reliability of the detection system. 相似文献
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Yinquan Fang Jun Yan Chenhui Li Xiao Zhou Lemeng Yao Tao Pang Ming Yan Luyong Zhang Lei Mao Hong Liao 《The Journal of biological chemistry》2015,290(48):28901-28914
Microglia have been proposed to play a pivotal role in the inflammation response of the CNS by expressing a range of proinflammatory enzymes and cytokines under pathological stimulus. Our previous study has confirmed that Nogo receptor (NgR), an axon outgrowth inhibition receptor, is also expressed on microglia and regulates cell adhesion and migration behavior in vitro. In the present study, we further investigated the proinflammatory effects and possible mechanisms of Nogo on microglia in vitro. In this study, Nogo peptide, Nogo-P4, a 25-amino acid core inhibitory peptide sequence of Nogo-66, was used. We found that Nogo-P4 was able to induce the expression of inducible nitric-oxide synthase and cyclooxygenase-2 and the release of proinflammatory cytokines, including IL-1β, TNF-α, NO, and prostaglandin E2 in microglia, which could be reversed by NEP1–40 (Nogo-66(1–40) antagonist peptide), phosphatidylinositol-specificphospholipase C, or NgR siRNA treatment. After Nogo-P4 stimulated microglia, the phosphorylation levels of NF-κB and STAT3 were increased obviously, which further mediated microglia expressing proinflammatory factors induced by Nogo-P4. Taken together, we concluded that Nogo peptide could directly take part in CNS inflammatory process by influencing the expression of proinflammatory factors in microglia, which were related to the NF-κB and STAT3 signal pathways. Besides neurite outgrowth restriction, the Nogo/NgR signal might be involved in multiple processes in various inflammation-associated CNS diseases. 相似文献
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Jianmin Wang Zhe Chen Linlin Bao Weijia Zhang Ying Xue XingHuo Pang Xi Zhang Chuan Qin Qi Jin 《Journal of virology》2015,89(17):9115-9118
H7N9 was a cause of significant global health concern due to its severe infection and approximately 35% mortality in humans. By screening a Fab antibody phage library derived from patients who recovered from H7N9 infections, we characterized two human monoclonal antibodies (HuMAbs), HNIgGD5 and HNIgGH8. The epitope of these two antibodies was dependent on two residues in the receptor binding site at positions V186 and L226 of the hemagglutinin glycoprotein. Both antibodies possessed high neutralizing activity. 相似文献
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