全文获取类型
收费全文 | 999篇 |
免费 | 64篇 |
国内免费 | 67篇 |
出版年
2023年 | 11篇 |
2022年 | 10篇 |
2021年 | 21篇 |
2020年 | 30篇 |
2019年 | 20篇 |
2018年 | 24篇 |
2017年 | 18篇 |
2016年 | 27篇 |
2015年 | 36篇 |
2014年 | 52篇 |
2013年 | 46篇 |
2012年 | 49篇 |
2011年 | 33篇 |
2010年 | 23篇 |
2009年 | 24篇 |
2008年 | 43篇 |
2007年 | 38篇 |
2006年 | 36篇 |
2005年 | 47篇 |
2004年 | 35篇 |
2003年 | 35篇 |
2002年 | 31篇 |
2001年 | 48篇 |
2000年 | 39篇 |
1999年 | 21篇 |
1998年 | 32篇 |
1997年 | 24篇 |
1996年 | 33篇 |
1995年 | 26篇 |
1994年 | 22篇 |
1993年 | 19篇 |
1992年 | 28篇 |
1991年 | 16篇 |
1990年 | 23篇 |
1989年 | 13篇 |
1988年 | 17篇 |
1987年 | 10篇 |
1986年 | 12篇 |
1985年 | 6篇 |
1984年 | 11篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 9篇 |
1980年 | 7篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1975年 | 1篇 |
1974年 | 3篇 |
1972年 | 1篇 |
排序方式: 共有1130条查询结果,搜索用时 31 毫秒
91.
The Golgi apparatus lies at the heart of the secretory pathway where it receives, modifies and sorts protein cargo to the proper intracellular or extracellular location. Although this secretory function is highly conserved throughout the eukaryotic kingdom, the structure of the Golgi complex is arranged very differently among species. In particular, Golgi membranes in vertebrate cells are integrated into a single compact entity termed the Golgi ribbon that is normally localized in the perinuclear area and in close vicinity to the centrosomes. This organization poses a challenge for cell division when the single Golgi ribbon needs to be partitioned into the two daughter cells. To ensure faithful inheritance in the progeny, the Golgi ribbon is divided in three consecutive steps in mitosis, namely disassembly, partitioning and reassembly. However, the structure of the Golgi ribbon is only present in higher animals and Golgi disassembly during mitosis is not ubiquitous in all organisms. Therefore, there must be unique reasons to build up the Golgi in this particular conformation and to preserve it over generations. In this review, we first highlight the diversity of the Golgi architecture in different organisms and revisit the concept of the Golgi ribbon. Following on, we discuss why the ribbon is needed and how it forms in vertebrate cells. Lastly, we conclude with likely purposes of mitotic ribbon disassembly and further propose mechanisms by which it regulates mitosis. 相似文献
92.
Recent debates about memetics have revealed some widespread misunderstandings about Darwinian approaches to cultural evolution.
Drawing from these debates, this paper disputes five common claims: (1) mental representations are rarely discrete, and therefore
models that assume discrete, gene-like particles (i.e., replicators) are useless; (2) replicators are necessary for cumulative,
adaptive evolution; (3) content-dependent psychological biases are the only important processes that affect the spread of
cultural representations; (4) the “cultural fitness” of a mental representation can be inferred from its successful transmission;
and (5) selective forces only matter if the sources of variation are random. We close by sketching the outlines of a unified
evolutionary science of culture.
Joseph Henrich (Ph.D. UCLA, 1999) holds the Canada Research Chair in Culture, Cognition, and Coevolution in the Departments of Psychology and Economics at the University of British Columbia. His research combines behavioral and cognitive experiments, in-depth field ethnography, and evolutionary modeling to explore the coevolutionary emergence of cooperative institutions, prosocial motivations, religions, and complex cultural adaptations. See his website at Robert Boyd received his bachelor’s degree in physics from the University of California at San Diego and a Ph.D. in ecology from UC Davis. He has taught at Duke and Emory universities and has been at UCLA since 1986. With Herb Gintis, Rob currently co-directs the MacArthur Research Network on the Nature and Origin of Preferences. His research focuses on population models of culture. Rob has also co-authored an introductory textbook in biological anthropology, How Humans Evolved, with his wife, Joan Silk. He and Joan have two children and live in Los Angeles. His hobbies are rock climbing and bicycling. Peter J. Richerson received undergraduate and graduate degrees in entomology and zoology at the University of California, Davis. He is currently Distinguished Professor in the Department of Environmental Science and Policy at UC Davis. His research focuses on the processes of cultural evolution, most of it co-authored with Robert Boyd. Their 1985 book applied the mathematical tools used by organic evolutionists to study a number of basic problems in human cultural evolution. His recent publications have used theoretical models to try to understand some of the main events in human evolution, such as the evolution of the advanced capacity for imitation (and hence cumulative cultural evolution) in humans, the origins of tribal and larger-scale cooperation, and the origins of agriculture. He collaborates with Richard McElreath and Mark Lubell in an NSF-funded research group devoted to the study of cultural transmission and cultural evolution in laboratory systems. 相似文献
Robert BoydEmail: |
Joseph Henrich (Ph.D. UCLA, 1999) holds the Canada Research Chair in Culture, Cognition, and Coevolution in the Departments of Psychology and Economics at the University of British Columbia. His research combines behavioral and cognitive experiments, in-depth field ethnography, and evolutionary modeling to explore the coevolutionary emergence of cooperative institutions, prosocial motivations, religions, and complex cultural adaptations. See his website at Robert Boyd received his bachelor’s degree in physics from the University of California at San Diego and a Ph.D. in ecology from UC Davis. He has taught at Duke and Emory universities and has been at UCLA since 1986. With Herb Gintis, Rob currently co-directs the MacArthur Research Network on the Nature and Origin of Preferences. His research focuses on population models of culture. Rob has also co-authored an introductory textbook in biological anthropology, How Humans Evolved, with his wife, Joan Silk. He and Joan have two children and live in Los Angeles. His hobbies are rock climbing and bicycling. Peter J. Richerson received undergraduate and graduate degrees in entomology and zoology at the University of California, Davis. He is currently Distinguished Professor in the Department of Environmental Science and Policy at UC Davis. His research focuses on the processes of cultural evolution, most of it co-authored with Robert Boyd. Their 1985 book applied the mathematical tools used by organic evolutionists to study a number of basic problems in human cultural evolution. His recent publications have used theoretical models to try to understand some of the main events in human evolution, such as the evolution of the advanced capacity for imitation (and hence cumulative cultural evolution) in humans, the origins of tribal and larger-scale cooperation, and the origins of agriculture. He collaborates with Richard McElreath and Mark Lubell in an NSF-funded research group devoted to the study of cultural transmission and cultural evolution in laboratory systems. 相似文献
93.
94.
Epigenetic inheritance in rice plants 总被引:2,自引:0,他引:2
BACKGROUND AND AIMS: Epigenetics is defined as mechanisms that regulate gene expression without base sequence alteration. One molecular basis is considered to be DNA cytosine methylation, which reversibly modifies DNA or chromatin structures. Although its correlation with epigenetic inheritance over generations has been circumstantially shown, evidence at the gene level has been limited. The present study aims to find genes whose methylation status directly correlates with inheritance of phenotypic changes. METHODS: DNA methylation in vivo was artificially reduced by treating rice (Oryza sativa ssp. japonica) seeds with 5-azadeoxycytidine, and the progeny were cultivated in the field for > 10 years. Genomic regions with changed methylation status were screened by the methylation-sensitive amplified polymorphysm (MSAP) method, and cytosine methylation was directly scanned by the bisulfite mapping method. Pathogen infection with Xanthomonas oryzae pv. oryzae, race PR2 was performed by the scissors-dip method on mature leaf blades. KEY RESULTS: The majority of seedlings were lethal, but some survived to maturity. One line designated as Line-2 showed a clear marker phenotype of dwarfism, which was stably inherited by the progeny over nine generations. MSAP screening identified six fragments, among which two were further characterized by DNA blot hybridization and direct methylation mapping. One clone encoding a retrotransposon gag-pol polyprotein showed a complete erasure of 5-methylcytosines in Line-2, but neither translocation nor expression of this region was detectable. The other clone encoded an Xa21-like protein, Xa21G. In wild-type plants, all cytosines were methylated within the promoter region, whereas in Line-2, corresponding methylation was completely erased throughout generations. Expression of Xa21G was not detectable in wild type but was constitutive in Line-2. When infected with X. oryzae pv. oryzae, against which Xa21 confers resistance in a gene-for-gene manner, the progeny of Line-2 were apparently resistant while the wild type was highly susceptible without Xa21G expression. CONCLUSIONS: These results indicated that demethylation was selective in Line-2, and that promoter demethylation abolished the constitutive silencing of Xa21G due to hypermethylation, resulting in acquisition of disease resistance. Both hypomethylation and resistant trait were stably inherited. This is a clear example of epigenetic inheritance, and supports the idea of Lamarckian inheritance which suggested acquired traits to be heritable. 相似文献
95.
Desert locusts demonstrate pronounced density-dependent polyphenism: a complex suite of traits shifts over the lifetime of an individual in response to crowding or isolation. These changes also accumulate across generations through a maternal effect. Female desert locusts alter the developmental trajectory of their offspring in response to their own experience of crowding. The mother possesses a memory of both the recency and extent of crowding and shifts the phase state of her hatchlings accordingly. Extensive experimental work has shown that offspring behaviour is controlled by a low molecular weight, polar compound (or compounds) released from the mother's accessory glands. The chemical identity of this agent is not yet known. 相似文献
96.
The organization of eukaryotic cells into membrane-bound compartments must be faithfully sustained for survival of the cell. A subtle equilibrium exists between the degradation and the proliferation of organelles. Commonly, proliferation is initiated by a membrane remodeling process. Here, we dissect the function of proteins driving organelle proliferation in the particular case of peroxisomes. These organelles are formed either through a growth and division process from existing peroxisomes or de novo from the endoplasmic reticulum (ER). Among the proteins involved in the biogenesis of peroxisomes, peroxins, members of the Pex11 protein family participate in peroxisomal membrane alterations. In the yeast Saccharomyces cerevisiae, the Pex11 family consists of three proteins, Pex11p, Pex25p and Pex27p. Here we demonstrate that yeast mutants lacking peroxisomes require the presence of Pex25p to regenerate this organelle de novo. We also provide evidence showing that Pex27p inhibits peroxisomal function and illustrate that Pex25p initiates elongation of the peroxisomal membrane. Our data establish that although structurally conserved each of the three Pex11 protein family members plays a distinct role. While ScPex11p promotes the proliferation of peroxisomes already present in the cell, ScPex25p initiates remodeling at the peroxisomal membrane and ScPex27p acts to counter this activity. In addition, we reveal that ScPex25p acts in concert with Pex3p in the initiation of de novo peroxisome biogenesis from the ER. 相似文献
97.
Bustamante-Aragonés A Rodríguez de Alba M Perlado S Trujillo-Tiebas MJ Arranz JP Díaz-Recasens J Troyano-Luque J Ramos C 《Gene》2012,504(1):144-149
Prenatal diagnosis (PD) is available for pregnancies at risk of monogenic disorders. However, PD requires the use of invasive obstetric techniques for fetal-sample collection and therefore, involves a risk of fetal loss. Circulating fetal DNA in the maternal bloodstream is being used to perform non-invasive prenatal diagnosis (NIPD). NIPD is a challenging discipline because of the biological features of the maternal blood sample. Maternal blood is an unequal mixture of small (and fragmented) amounts of fetal DNA within a wide background of maternal DNA. For this reason, initial NIPD studies have been based on the analysis of specific paternally inherited fetal tracts not present in the maternal genome so as to ensure their fetal origin. Following this strategy, different NIPD studies have been carried out, such as fetal-sex assessment for pregnancies at risk of X-linked disorders, RhD determination, and analysis of single-gene disorders with a paternal origin. The study of the paternal mutation can be used for fetal diagnosis of dominant disorders or to more accurately assess the risk of an affected child in case of recessive diseases. Huntington's disease, cystic fibrosis, or achondroplasia are some examples of diseases studied using NIPD. New technologies are opening NIPD to the analysis of maternally inherited fetal tracts. NIPD of trisomy 21 is the latest study derived from the use of next-generation sequencing (NGS). 相似文献
98.
Results from the analysis of copy number variations (CNVs) in human pluripotent cell-derived neuroprogenitor cell lines (hiPSC and hESC-derived NPC) are presented. Two different types of CNVs were detected: a) CNVs inherited from the original source of pluripotent cells (hESC and hiPSC) and b) CNVs detected either in the original source of pluripotent cells or in the derived NPC cell lines but not in both at the same time. Our data suggest that submicroscopic chromosomal changes happened during culture and manipulation of cells and those differentiation procedures could result in gains and losses of genomic regions in pluripotent cell-derived neuroprogenitors. Overall, the results indicate that even chromosomally stable stem cell lines would need to be analyzed in detail by high resolution methodologies before their clinical use. 相似文献
99.
Objectives
Amelogenesis imperfecta, dentinogenesis imperfecta, and dentin dysplasia are the most common non-syndromic dental disorders. In this study, we analysed and localised the gene(s) responsible for inherited non-syndromic dental disorders in a Chinese family.Methods
This study identified and researched non-syndromic dental disorders in a four-generation Chinese family, including four affected individuals whose clinical phenotype was atypical. Linkage analysis with seven polymorphic markers that localise to six different autochromosomes showed that the family was linked through chromosome 4q. All exons and exon–intron boundaries of dentin sialophosphoprotein (DSPP), enamelin (ENAM), and ameloblastin (AMBN), which are located on chromosome 4q, were sequenced in nine of the family members.Results
Direct DNA sequence analysis revealed the existence of a G to A transversion in exon 4 (g.13081786G > A, c.727G > A, p.Asp243Asn, based on reference sequences NM_014208.3) of the DSPP gene, and this sequence variation correlated exactly with the presence of the disease.Conclusion
These results indicate that mutation p.Asp243Asn is a highly probable cause of non-syndromic dental disorder in this Chinese family. The presence of symptom heterogeneity is possible, as the clinical classification system is hampered by the lack of close correlation between the subtype and the molecular defect. 相似文献100.
Irene E. Zohn 《Birth defects research. Part C, Embryo today : reviews》2012,96(2):193-205
Neural tube defects (NTDs) such as spina bifida and anencephaly are some of the most common structural birth defects found in humans. These defects occur due to failures of neurulation, a process where the flat neural plate rolls into a tube. In spite of their prevalence, the causes of NTDs are poorly understood. The multifactorial threshold model best describes the pattern of inheritance of NTDs where multiple undefined gene variants interact with environmental factors to cause an NTD. To date, mouse models have implicated a multitude of genes as required for neurulation, providing a mechanistic understanding of the cellular and molecular pathways that control neurulation. However, the majority of these mouse models exhibit NTDs with a Mendelian pattern of inheritance. Still, many examples of multifactorial inheritance have been demonstrated in mouse models of NTDs. These include null and hypomorphic alleles of neurulation genes that interact in a complex fashion with other genetic mutations or environmental factors to cause NTDs. These models have implicated several genes and pathways for testing as candidates for the genetic basis of NTDs in humans, resulting in identification of putative pathogenic mutations in some patients. Mouse models also provide an experimental paradigm to gain a mechanistic understanding of the environmental factors that influence NTD occurrence, such as folic acid and maternal diabetes, and have led to the discovery of additional preventative nutritional supplements such as inositol. This review provides examples of how multifactorial inheritance of NTDs can be modeled in the mouse. Birth Defects Research (Part C) 96:193–205, 2012. © 2012 Wiley Periodicals, Inc. 相似文献