Development of the leafy shoot in Sphagnum (Bryophyta) involves the activity of both apical and subapical meristems

This light- and electron-microscope study of four species of Sphagnum reveals that stem elongation involves meristematic activities unique to the group and hitherto unrecognized. The internal tissue of the mature stem arises by the concerted activity of an apical (primary) and a subapical (secondary) meristem. The primary meristem comprises the immediate derivatives of the single apical cell. Following a small number of divisions, the primary derivatives differentiate into highly vacuolate parenchymatous cells with a storied arrangement. Subsequently, the large vacuoles are replaced by numerous small vacuoles and the cells then divide repeatedly, by transverse septa, producing files of about nine short cells. Finally, ninefold elongation of these secondary cells is responsible for extension growth of the main stem below the mature capitulum. An early step in primary differentiation is the confinement of pre-existing plasmodesmata to distinct pitted areas. Further enlargement of the cells during primary and secondary differentiation involves the thickening of non-pitted wall areas, followed by expansion and thinning out, while the pitted areas remain virtually unchanged. A cortical array of microtubules is regularly found in association with non-pitted wall areas, while the unexpanded pitted areas are associated with smooth endoplasmic reticulum showing continuity with desmotubules. Though sharing much the same cytology as the conducting cells in bryoid mosses, in terms of their development the central stem cells in Sphagnum are not homologous with those of other mosses. The unique mode of stem development may be an important factor in the ecological success of Sphagnum .     

The tardigrades of Emilia (Italy). II. Monte Rondinaio. A multihabitat study on a high altitude valley of the northern Apennines

We investigated a small valley of glacial origin in the northern Apennines (Italy). A multihabitat study was performed on the mosses and lichens on rocks, temporarily submersed mosses, beech litter, grass turf and sediments from pools, ponds and streams. A few species colonized more than one habitat. Several species were typical of high altitudes or latitudes, some were rare, and two, Hypsibius pradellii sp. nov. and Diphascon (Diphascon) secchii sp. nov. , were new to science.     

Worker laying in leafcutter ant Acromyrmex subterraneus brunneus （Formicidae, Attini）

We studied the process of offspring production in queenless colonies of Acromyrmex subterraneus brunneus, and particularly evaluated the ovary development of workers as a function of their age. For this, subcolonies were set up and evaluated at different periods of isolation from the queen （2, 4 and 6 months）, besides individually labeled age groups. The subcolonies were assessed according to offspring production and ovaries containing oocytes or not. The evaluations showed worker oviposition and development of males originating from worker-laid eggs. At 2 months＇ absence of the queen, eggs and larvae were found, with eggs in a higher proportion than larvae. After 4 months, the proportion of eggs had reduced while larvae had increased, and a pupa was found in one subcolony. At 6 months, besides a higher share of larvae, one pupa and one adult male were found. Dissection of workers revealed ovaries containing oocytes during the periods of evaluation. Only a group of medium-sized and large workers, 23.3%, 20.9% and 37.5% of the population from each period assessed in queenless subcolonies respectively, presented developed oocytes in the ovary. The same was observed in colonies with a queen, with 17.6%, 19.6% and 7.8% of the group of dissected workers from each time period, respectively. With respect to worker age, we observed by dissection of the ovary, that the greatest percentage of individuals with ovarioles containing oocytes occurred at 45 days （6 weeks） up to 90 days （12 weeks）. These results probably are associated with the workers reproduction and the laying of trophic and reproductive eggs in colonies with and without a queen; these eggs have distinct functions in each situation.     

Effect of a postfire environment on the establishment of Prosopis caldenia seedlings in central semiarid Argentina

Abstract The objective of this work was to evaluate postfire environmental effects on the emergence, survival and growth of Prosopis caldenia seedlings in relation to different controlled fire frequencies, seed scarification methods, and planting site. Seedling emergence was significantly higher in experimental units exposed twice and three times to controlled fire than in unburned experimental units. The highest average seedling survival was recorded with triple exposure to controlled fires. Emergence, survival and growth of seedlings from seeds exposed to acid scarification and 600°C for 5 min were higher in the burned experimental units than in the unburned ones. In the former, seedling survival was higher beneath a P. caldenia canopy than in an adjacent open site, though seedling emergence was similar in both planting sites. Our results suggest that postfire conditions characterized by a reduction in the vegetative cover and competition interference and an increase in soil temperatures and nutrients levels (e.g. nitrogen and phosphorus) may facilitate the establishment of P. caldenia seedlings in the Caldenal.     

Protein-Synthesizing Machinery in the Growing Oocyte of the Cyclic Mouse

A quantitative ultrastructural evaluation of the oocyte ribosomal population was carried out during the oocyte growth, bearing in mind that this period of the mouse oogenesis displays the greatest activity of ribosomal RNA synthesis.
At the onset of growth almost 3/4 of the oocyte ribosomes exist as singles, these become polysomal ribosomes as growth progresses. At the same time the number of ribosomes increases. Once the major growth period has elapsed, the number of ribosomes starts to decrease just when lattice-like structures exhibiting a periodic organization begin to accumulate in the oocyte cytoplasmic matrix.
Evidence, like the particulate organization of these lattices, the size of their particles, its digestion by RNase, and the time of the lattice appearance, together with data reported by several authors, allows one to suggest that near the end of the oocyte growth a great part of the ribosomes are stored in the lattices to be used during early development.     

Ultrastructure of the Developing and Fertilized Embryo Sac of Amaranthus hypochondriacus L.

Amaranthus hypochondriacus embryo sac development was investigatedbefore and after fertilization. During the early stages of development,the young embryo sac displays three antipodal cells at the chalazalpole that degenerate very early in the maturation process, beforethe synergids and egg cell are completely differentiated. Themature embryo sac is composed only of the female germ unit.The synergid cells organize a filiform apparatus accompaniedby the presence of mitochondria and dictyosomes with numerousvesicles. The involvement of the synergids in transport andsecretory functions related to pollen tube attraction and guidance,are discussed. The egg cell is located at the micropylar polenear the synergids and displays exposed plasma membranes atthe chalazal pole. The fertilized egg cell does not exhibitmarked changes after fertilization except for the closure ofthe cell wall. The central cell is the largest cell of thisvery long embryo sac. The fused nucleus is close to the eggapparatus before fertilization and displays a remarkable chalazalmigration after gamete delivery. The ultrastructure of the centralcell cytoplasm and the numerous wall ingrowths around this cellsuggest an important role in nutrient transportation. Aftergamete delivery, the embryo sac displays electron dense bodiesthat aggregate within the intercellular space between the synergids,egg cell and central cell. These bodies, that appear in theembryo sac of several plants, are probably involved in gametedelivery for double fertilization. The possibility of biparentalinheritance of mitochondria in this plant is also discussed.Copyright 1999 Annals of Botany Company Amaranthus hypochondriacus, grain amaranth, embryo sac, fertilization.     

Nutrient content affecting Spodoptera frugiperda and Dalbulus maidis occurrence in corn

This work aims to study the effect of the nutrient contents in the leaves of corn on the incidence of Spodopterafrugiperda （Smith） （Lepidoptera： Noctuidae） and Dalbulus maidis （Delong ＆ Wolcott） （Homoptera： Cicadellidae）. The treatments were represented by unfertilized corn plants and corn plants fertilized with 250 kg of the formulation 4-14-8 （N- P-K） ＋ 100 kg of ammonium sulfate （AS）/ha and 500 kg of the formulation 4-14-8 （N-P-K） ＋200 kg of AS/ha, arranged in randomized blocks with four replicates. Attacks by S. frugiperda and D. maidis were higher as the nitrogen, sulfur, calcium and copper content in the leaves of corn increased. The effects of calcium and copper found in this study were not consistent with that of lower susceptibility associated with higher levels of these nutrients mentioned in the literature. We believe that there was a cumulative effect of the nutrients altogether, building up a nutritiously better vegetable which in turn had less reserve for defense, or that the content of both nutrients in the leaves were not high enough to induce such effects.