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61.
Dispersal and philopatry influence gene flow and thus the spatio-genetic structure within and between populations. In callitrichids the flexible social and mating system corresponds with a variable migration pattern where both sexes might be philopatric or might disperse. We investigated the relationship between the spatio-genetic structure and migration patterns in a population of mustached tamarins, Saguinus mystax. Using the rapidly evolving hypervariable region I (HVI) of the mitochondrial control region and 11 microsatellite markers we detected a high variation (HVI: 16 haplotypes in 69 individuals; microsatellites: H(O) = 0.75, average: 7.45 alleles/locus), with mating partners usually not sharing the same haplotype, indicating that matings are generally between partners that are not closely related. Similar high variance of haplotype differences for male-male and female-female pairs, along with a slightly higher number of haplotype differences in males show that both sexes habitually migrate. Spatial analyses suggest that females usually migrate longer distances, corresponding to very limited breeding positions for females in a polyandrous social mating system. 相似文献
62.
Chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) were studied in 24 populations of Prunus spinosa sampled across Europe. The cpDNA and mtDNA fragments were amplified using universal primers and subsequently digested with restriction enzymes to obtain the polymorphisms. Combinations of all the polymorphisms resulted in 33 cpDNA haplotypes and two mtDNA haplotypes. Strict association between the cpDNA haplotypes and the mtDNA haplotypes was detected in most cases, indicating conjoint inheritance of the two genomes. The most frequent and abundant cpDNA haplotype (C20; frequency, 51 %) is always associated with the more frequent and abundant mtDNA haplotype (M1; frequency, 84 %). All but two of the cpDNA haplotypes associated with the less frequent mtDNA haplotype (M2) are private haplotypes. These private haplotypes are phylogenetically related but geographically unrelated. They form a separate cluster on the minimum-length spanning tree. 相似文献
63.
64.
Virulence of Beauveria brongniartii and B. bassiana against Schizonycha affinis white grubs and adults (Coleoptera: Scarabaeidae) 下载免费PDF全文
Two endemic scarab pests, Schizonycha affinis Boheman and Hypopholis sommeri Burmeister (Coleoptera: Melolonthinae) have increased in prevalence in the sugarcane producing regions of the KwaZulu‐Natal Midlands, South Africa. The crop losses associated with their feeding, the failure of chemical insecticides applied for their control, and the recent discovery of Beauveria brongniartii (Sacc.) Petch (Ascomycota: Cordycipitaceae) epizootics on these pests, have generated interest in the development of a mycoinsecticide targeting adults and larvae of these species. Previous research, using microsatellite markers, identified low levels of genetic diversity among isolates of B. brongniartii collected from two field sites where epizootics occurred. The virulence of 21 of these closely related B. brongniartii isolates and two isolates of Beauveria bassiana (Balsamo) Vuillemin was evaluated. Bioassays were conducted against adults and larvae of S. affinis, and adult Tenebrio molitor (L.) (Coleoptera: Tenebrionidae) as a surrogate test insect. The closely related B. brongniartii isolates varied significantly in their virulence towards both S. affinis (50.1–95% mortality) and T. molitor (39–74% mortality), with a number of these not highly virulent against either of these insect species. Those isolates sharing a haplotype did not vary in virulence. Adults of S. affinis were more susceptible than larvae to isolates of B. brongniartii. The median lethal concentration (LC50) required to kill half the adult S. affinis test insects was 7.65 × 106 conidia per millilitre. Schizonycha affinis second instar larvae had a median survival time of 17.5 days when exposed to some B. brongniartii isolates; however, third instars survived significantly longer with a median of 21 days. Third instars exposed to the highest concentration of B. brongniartii isolate HHWG1 survived for a median time of 15 days. Bioassays supported the finding that genetically closely related isolates may vary in their virulence, even if they were obtained from the same field epizootics. 相似文献
65.
POPULATION STRUCTURE AND PHYSIOLOGICAL DIFFERENTIATION OF HAPLOTYPES OF CALOGLOSSA LEPRIEURII (RHODOPHYTA) IN A MANGROVE INTERTIDAL ZONE 总被引:2,自引:0,他引:2
Giuseppe C. Zuccarello Paul H. Yeates Jeff T. Wright Jason Bartlett 《Journal of phycology》2001,37(2):235-244
Zonation of macroalgae in the intertidal zone has been well documented. However, studies of zonation of macroalgae have predominantly examined the distribution of different species rather than the distribution of variants within a species. This study investigated the spatial variation of plastid haplotypes of the mangrove red alga Caloglossa leprieurii (Montagne) J. Agardh at a site in eastern Australia and tests for physiological differences (growth, photosynthesis) between those haplotypes. RUBISCO spacer plastid haplotypes were scored using single-stranded comformational polymorphism, and the population structure at two sites was examined using a nested sampling design comparing between sites, among transects within sites, and among quadrats within transects. Growth rates at various salinities and light intensities and the photosynthesis–irradiance curves of the three main haplotypes were compared. The two sites showed a high degree of genetic differentiation across a short distance, suggesting limited gene flow. The distribution of haplotypes was patchy and did not reflect a zonation pattern along the intertidal gradient. The three haplotypes were physiologically differentiated with haplotype A, with a lower growth rate and a lower photosynthetic efficiency at higher light intensities. There is some evidence of physiological differentiation between life history phases in C. leprieurii with sporophytes having a higher growth rate than females under most conditions. Our results suggest a correlation between our culture results and our population data. Haplotypes (haplotype A) and life history phases (gametophytes) with lower performance (growth and photosynthetic efficiency) under our culture conditions were correlated with a minor representation in the field. This is the first study to integrate population-level data with physiological parameters toward an understanding of the distribution and relative abundance of red algal genetic variants. 相似文献
66.
We sequenced 556 bp of the mitochondrial ND5 gene to infer aspects of population structure and to test subspecific designations of argali sheep (Ovis ammon) in Mongolia. Analysis of molecular variance (AMOVA) revealed greater variation within than among putative subspecies and populations, suggesting high levels female-mediated gene flow. Compared with bighorn sheep (O. canadensis) in North America, substantially less differentiation in mitochondrial DNA was found among argali populations over 1200 km than was found among bighorn populations over 250 km. This result is consistent with differences in argali and bighorn life history traits. Argali run for long distances across open terrain in the presence of a threat rather than running up into steep escape terrain like bighorn sheep do. Our results suggest recognizing only one Evolutionary Significant Unit (subspecies) of argali in Mongolia, but they may support recognizing two Management Units, because two regions do exhibit slightly different haplotype frequencies at the ND5 gene of mtDNA. 相似文献
67.
Orissa, on the east coast of India, is one of the three mass nesting sites in the world for olive ridley turtles (Lepidochelys olivacea). This population is currently under threat as a result of fishery-related mortality; more than 100 000 olive ridleys have been counted dead in the last 10 years in Orissa. In general, the globally distributed olive ridley turtle has received significantly less conservation attention than its congener, the Kemp's ridley turtle (L. kempi), because the latter is recognized as a distinct species consisting of a single endangered population. Our study of mitochondrial DNA haplotypes suggests that the ridley population on the east coast of India is panmictic, but distinct from all other populations including Sri Lanka. About 96% of the Indian population consisted of a distinct 'K' clade with haplotypes not found in any other population. Nested clade analysis and conventional analysis both supported range expansions and/or long-distance colonization from the Indian Ocean clades to other oceanic basins, which suggested that these are the ancestral source for contemporary global populations of olive ridley turtles. These data support the distinctiveness of the Indian Ocean ridleys, suggesting that conservation prioritization should be based on appropriate data and not solely on species designations. 相似文献
68.
European Y-chromosomal lineages in Polynesians: a contrast to the population structure revealed by mtDNA. 总被引:10,自引:3,他引:7 下载免费PDF全文
M E Hurles C Irven J Nicholson P G Taylor F R Santos J Loughlin M A Jobling B C Sykes 《American journal of human genetics》1998,63(6):1793-1806
We have used Y-chromosomal polymorphisms to trace paternal lineages in Polynesians by use of samples previously typed for mtDNA variants. A genealogical approach utilizing hierarchical analysis of eight rare-event biallelic polymorphisms, seven microsatellite loci, and internal structural analysis of the hypervariable minisatellite, MSY1, has been used to define three major paternal-lineage clusters in Polynesians. Two of these clusters, both defined by novel MSY1 modular structures and representing 55% of the Polynesians studied, are also found in coastal Papua New Guinea. Reduced Polynesian diversity, relative to that in Melanesians, is illustrated by the presence of several examples of identical MSY1 codes and microsatellite haplotypes within these lineage clusters in Polynesians. The complete lack of Y chromosomes having the M4 base substitution in Polynesians, despite their prevalence (64%) in Melanesians, may also be a result of the multiple bottleneck events during the colonization of this region of the world. The origin of the M4 mutation has been dated by use of two independent methods based on microsatellite-haplotype and minisatellite-code diversity. Because of the wide confidence limits on the mutation rates of these loci, the M4 mutation cannot be conclusively dated relative to the colonization of Polynesia, 3,000 years ago. The other major lineage cluster found in Polynesians, defined by a base substitution at the 92R7 locus, represents 27% of the Polynesians studied and, most probably, originates in Europe. This is the first Y-chromosomal evidence of major European admixture with indigenous Polynesian populations and contrasts sharply with the picture given by mtDNA evidence. 相似文献
69.
We review our studies of mate choice with two MHC‐congenic strains of mice. This work was stimulated by findings from Yamazaki
and colleagues showing that male mice exhibited mate preferences for females whose MHC‐haplotype was different from their
own, while female mice exhibited either no preference or a weak preference for males of a particular MHC‐haplotype (see Beauchamp
et al., 1988). Since these findings were unexpected (mate choice theory predicts that females will be more selective than
males), we studied the preferences of mice from two additional MHC‐congenic strains to assess the generality of the previous
findings. Specifically, the goals of our research were: (1) to determine the mate preferences of congenic mice with MHC‐haplotypes
derived from wild populations, (2) to compare the mate preferences of male and female mice in a test situation where each
sex has a clear opportunity to make a choice, and (3) to estimate effects of cross‐fostering on each sex.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
70.
Thierry Gaude Mireille Rougier Philippe Heizmann David J. Ockendon Christian Dumas 《Plant molecular biology》1995,27(5):1003-1014
In Brassica, the S-locus glycoprotein (SLG) gene has been strongly implicated in the self-incompatibility reaction. Several alleles of this locus have been sequenced, and accordingly grouped as class I (corresponding to dominant S-alleles) and class II (recessive). We recently showed that a self-compatible (Sc) line of Brassica oleracea expressed a class II-like SLG (SLG-Sc) gene. Here, we report that the SLG-Sc glycoprotein is electrophoretically and immunochemically very similar to the recessive SLG-S15 glycoprotein, and is similarly expressed in stigmatic papillae. Moreover, by seed yield analysis, we observe that both alleles are associated with a self-compatibility response, in contrast with the other known recessive S haplotypes (S2 and S5). By genomic DNA blot analysis, we show the existence of molecular homologies between the Sc and S15 haplotypes, but demonstrate that they are not identical. On the other hand, we also report that the S2 haplotype expresses very low amounts of SLG glycoproteins, although it exhibits a self-incompatible phenotype. These results strongly question the precise role of the SLG gene in the molecular mechanisms that control the self-incompatibility reaction of Brassica. 相似文献