全文获取类型
收费全文 | 195篇 |
免费 | 25篇 |
出版年
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 3篇 |
2013年 | 5篇 |
2012年 | 9篇 |
2011年 | 4篇 |
2010年 | 5篇 |
2009年 | 9篇 |
2008年 | 14篇 |
2007年 | 9篇 |
2005年 | 7篇 |
2004年 | 5篇 |
2003年 | 7篇 |
2002年 | 8篇 |
2001年 | 8篇 |
2000年 | 11篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1990年 | 8篇 |
1989年 | 2篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 2篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 4篇 |
1975年 | 8篇 |
1974年 | 4篇 |
1973年 | 6篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 4篇 |
1967年 | 3篇 |
1964年 | 2篇 |
1963年 | 2篇 |
排序方式: 共有220条查询结果,搜索用时 203 毫秒
51.
Karl-Heinz Riemann 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1963,33(5):217-226
Ohne ZusammenfassungMit 4 Abbildungen 相似文献
52.
Kunxi Zhang Xin Zhu Steffen Durst Petra Hohenberger Min‐Jung Han Gynheung An Vaidurya P. Sahi Michael Riemann Peter Nick 《植物学报(英文版)》2021,63(5):848-864
The detyrosination/retyrosination cycle is the most common post‐translational modification of α‐tubulin. Removal of the conserved C‐terminal tyrosine of α‐tubulin by a still elusive tubulin tyrosine carboxypeptidase, and religation of this tyrosine by a tubulin tyrosine ligase (TTL), are probably common to all eukaryotes. Interestingly, for plants, the only candidates qualifying as potential TTL homologs are the tubulin tyrosine ligase‐like 12 proteins. To get insight into the biological functions of these potential TTL homologs, we cloned the rice TTL‐like 12 protein (OsTTLL12) and generated overexpression OsTTLL12‐RFP lines in both rice and tobacco BY‐2 cells. We found, unexpectedly, that overexpression of this OsTTLL12‐RFP increased the relative abundance of detyrosinated α‐tubulin in both coleoptile and seminal root, correlated with more stable microtubules. This was independent of the respective orientation of cortical microtubule, and followed by correspondingly changing growth of coleoptiles and seminal roots. A perturbed organization of phragmoplast microtubules and disoriented cell walls were further characteristics of this phenotype. Thus, the elevated tubulin detyrosination in consequence of OsTTLL12 overexpression affects structural and dynamic features of microtubules, followed by changes in the axiality of cell plate deposition and, consequently, plant growth. 相似文献
53.
54.
55.
Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule
femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm
and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects
to the overlying or underlying layers. Activated keratocytes in the laser-treated corneas were detected with two-photon imaging
postoperatively. Intratissue femtosecond laser ablation thus has potential as a effective technique in refractive surgery
for the treatment of visual disorders.
This work was supported in part by the German Science Foundation. 相似文献
56.
Large Variabilities in Host Strain Susceptibility and Phage Host Range Govern Interactions between Lytic Marine Phages and Their Flavobacterium Hosts 下载免费PDF全文
Karin Holmfeldt Mathias Middelboe Ole Nybroe Lasse Riemann 《Applied microbiology》2007,73(21):6730-6739
Phages are a main mortality factor for marine bacterioplankton and are thought to regulate bacterial community composition through host-specific infection and lysis. In the present study we demonstrate for a marine phage-host assemblage that interactions are complex and that specificity and efficiency of infection and lysis are highly variable among phages infectious to strains of the same bacterial species. Twenty-three Bacteroidetes strains and 46 phages from Swedish and Danish coastal waters were analyzed. Based on genotypic and phenotypic analyses, 21 of the isolates could be considered strains of Cellulophaga baltica (Flavobacteriaceae). Nevertheless, all bacterial strains showed unique phage susceptibility patterns and differed by up to 6 orders of magnitude in sensitivity to the same titer of phage. The isolated phages showed pronounced variations in genome size (8 to >242 kb) and host range (infecting 1 to 20 bacterial strains). Our data indicate that marine bacterioplankton are susceptible to multiple co-occurring phages and that sensitivity towards phage infection is strain specific and exists as a continuum between highly sensitive and resistant, implying an extremely complex web of phage-host interactions. Hence, effects of phages on bacterioplankton community composition and dynamics may go undetected in studies where strain identity is not resolvable, i.e., in studies based on the phylogenetic resolution provided by 16S rRNA gene or internal transcribed spacer sequences. 相似文献
57.
58.
59.
Increased degradation of extracellular matrix structures of lacrimal glands implicated in the pathogenesis of Sj?gren's syndrome. 总被引:1,自引:0,他引:1
Katja Schenke-Layland Jiansong Xie Ekaterini Angelis Barry Starcher Kaijin Wu Iris Riemann W Robb MacLellan Sarah F Hamm-Alvarez 《Matrix biology》2008,27(1):53-66
Lacrimal glands (LGs) of male non-obese diabetic (NOD) mice display many features of human LGs in patients afflicted with the autoimmune disease Sj?gren's syndrome (SS), including the loss of secretory functions and a lymphocytic infiltration into the glands by 4 months of age. So far, research has mainly focused on the intracellular events that are involved in initiating LG dysfunction; however, the impact of SS on extracellular matrix (ECM) structures of the diseased LGs has not yet been determined. In this study we identified and compared LG ECM formation and integrity of age-matched male healthy (BALB/c) and diseased (NOD) mice. LG tissues were examined using routine histological, biochemical, immunohistochemical and gene expression analysis. Multiphoton imaging and second-harmonic generation (SHG) microscopy permitted the non-invasive analysis of major LG ECM structures including collagen- and elastin-containing fibers. Biochemical testing demonstrated a significant loss of collagen, glycosaminoglycans and desmosine in NOD LGs when compared to healthy BALB/c LGs. Immunohistochemical staining and gene expression analysis confirmed this disease-related alteration of LG ECM structures. Furthermore, laser-induced autofluorescence and SHG microscopy revealed dramatic changes in the structural organization of most collagenous and elastic fibers of the diseased LG tissues that were more pronounced than those displayed by histological analysis. Our results clearly show an enhanced degradation of ECM proteins accompanied by the severe disorganization and deformation of ECM structures of diseased LG tissues. These new insights into the involvement of ECM degradation in SS may lead to novel therapies for patients suffering from dry eye disease. 相似文献
60.