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目的:针对下一代测序数据量大、序列长度短的特点,研究数据分析和质量评估方法。方法:选择已发布的Illumina-Solexa平台测序数据为研究对象,通过MAQ软件将测序数据与人类全基因组序列进行比对,并以外显子区域为例,在位点水平对测序数据质量进行评估。结果:结合已有软件系统和本文自创线性算法,建立了一套包括比对、拼接在内的测序数据质量评估系统。比对分析后,发现原始测序序列共覆盖了127,113,378个位点,涉及24条染色体上的64868个外显子。其中,每个位点都被测到的外显子为0.50%,位点平均测序深度大于等于1的外显子为3.98%。结论:成功构建了基于Illumina-Solexa测序平台的数据分析和质量评估方法,其可适用于其它第二代测序平台。研究者可在质量评估的基础上完善测序试验设计,并进行SNP和突变筛选及后续功能性研究。 相似文献
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Guangyong Zheng ;Hong Li ;Chuan Wang ;Quanhu Sheng ;Haiwei Fan ;Shaoyou Yang ;Boshu Liu ;Jianliang Dai ;Rong Zeng ;Lu Xie 《Acta biochimica et biophysica Sinica》2009,(4):273-279
With the development of functional genomics research, large-scale proteomics studies are now widespread, presenting significant challenges for data storage, exchange, and analysis. Here we present the Integrated Proteomics Exploring Database (IPED) as a platform for managing proteomics experimental data (both process and result data). IPED is based on the schema of the Proteome Experimental Data Repository (PEDRo), and complies with the General Proteomics Standard (GPS) drafted by the Proteomics Standards Committee of the Human Proteome Organization. In our work, we developed three components for the IPED platform: the IPED client editor, IPED server software, and IPED web interface. The client editor collects experimental data and generates an extensible markup language (XML) data file compliant with PEDRo and GPS; the server software parses the XML data file and loads information into a core database; and the web interface displays experimental results, to provide a convenient graphic representation of data. Given software convenience and data abundance, IPED is a powerful platform for data exchange and presents an important resource for the proteomics community. In its current release, IPED is available at http://www. biosino.org/iped2. 相似文献
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螺旋CT三维重建技术在胫骨平台骨折的诊疗应用分析 总被引:1,自引:0,他引:1
目的探讨螺旋CT三维重建在胫骨平台骨折中的临床应用。方法回顾性分析2007-2008年55例胫骨平台骨折病例.手术前均常规行X线摄片,行螺旋CT三维重建并与术中所见对照。结果所有病例螺旋CT三维重建明确地显示了骨折,并在骨折的分型和关节面碎裂情况的描述上较平片优越,与手术中所见一致。结论CT多种成像后处理技术可清晰显示骨折的部位、骨折线的走行方向、骨质碎裂程度和移位的距离以及关节面塌陷情况等,对术前分型、制订手术治疗方案具有重要的参考价值。 相似文献
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国际水稻基因组测序计划(IEGSP)顺利完成, 水稻基因的研究也进入了后基因组研究阶段. 水稻基因芯片数据注释分析是一项重要的功能基因组学研究内容, 它为理解水稻基因的生物学意义提供了帮助. 本研究开发了一个基于Web的水稻基因芯片数据注释和分析平台(RiceChip), 它比同类的注释数据库更加全面快捷. 本平台共由5个功能模块组成: BioChip模块为水稻基因表达数据提供快速检索和高级检索, 可依次按照Probe Set ID, Locus ID, Analysis Name等字段进行检索; BioAnno模块整合多个生物学数据库, 为水稻基因提供基因功能、蛋白质结构、生物代谢途径以及转录调控等方面的注释信息; BioSeq模块则收集水稻基因组的序列信息, 支持对水稻基因与芯片探针的序列查询; BioView模块是系统图形可视化的核心模块, 提供友好的访问界面与结果输出, 方便研究人员使用; BioAnaly模块结合R/Bioconductor统计分析工具提供高通量芯片数据的在线分析. 本系统从不同的方面依次提供了数据检索、基因注释、序列分析、数据可视化和数据分析等功能, 其数据收集的全面性与功能分析的强大性在同类水稻基因芯片数据注释和分析平台中都较突出. 相似文献
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杭州师范大学"实验动物科学实验室"由校实验动物中心和医学实验中心优化组合而成,隶属于杭州师大学实验室与设备管理处;2006年12月被确定为校级重点实验室,2008年12月被确定为杭州市重点实验室建设点;是浙江省首批实验动物公共服务平台成员单位--浙江省大动物实验基地及浙江省唯一的实验动物技术人员培训基地. 相似文献
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Yun-Jia Ning ;Manli Wang ;Maping Deng ;Shu Shen ;Wei Liu ;Wu-Chun Cao ;Fei Deng ;Yan-Yi Wang ;Zhihong Hu ;Hualin Wang 《分子细胞生物学报》2014,(4):324-337
For antiviral signaling mediated by retinoic acid-inducible gene I (RiG-I)-like receptors (RLRs), the recruitment of cytosoUc RLRs and downstream molecules (such as TBK1 and IKKε) to mitochondriaL platform is a central event that facilitates the establishment of host antiviral state. Here, we present an example of viral targeting for immune evasion through spatial isolation of TBK1/IKKε from mitochond riai antiviral platform, which was employed by severe fever with thrombocytopenia syndrome virus (SFTSV), a deadly bunyavirus emerging recently. We showed that SFTSV nonstructural protein NSs functions as the interferon (IFN) antagonist, mainly via suppressing TBK1/IKKε-IRF3 signaling. NSs mediates the formation of cytoplasmic inclusion bodies (IBs), and the blockage of IB formation impairs IFN-inhibiting activity of NSs. We next demonstrate that I Bs are utilized to compartmentalize TBK1/I KKε. The compartmentalization results in spatial isolation of the kinases from mitochondria, and deprived TBK1/IKKε may participate in antiviral complex assembly, leadingto the blockage of lFN ind uction. This study proposes a new role of viral I Bs as virus-built'jail' for imprisoning cellular factors and presents a novel and likely common mechanism of viral immune evasion through spatial isolation of critical signaling molecules from the mitochondrial antiviral platform. 相似文献
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