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21.
22.
Michael E. Salvucci Archie R. Portis Jr. William L. Ogren 《Photosynthesis research》1985,7(2):193-201
Ribulosebisphosphate carboxylase/oxygenase (EC 4.1.1.39) (rubisco) must be fully activated in order to catalyze the maximum rates of photosynthesis observed in plants. Activation of the isolated enzyme occurs spontaneously, but conditions required to observe full activation are inconsistent with those known to occur in illuminated chloroplasts. Genetic studies with a nutant of Arabidopsis
thaliana incapable of activating rubisco linked two chloroplast polypeptides to the activation process in vivo. Using a reconstituted light activation system, it was possible to demonstrate the participation of a chloroplast protein in rubisco activation. These results indicate that a specific chloroplast enzyme, rubisco activase, catalyzes the activation of rubisco in vivo. 相似文献
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Possible mechanisms for the inhibition of photosynthesis by ozone 总被引:20,自引:0,他引:20
Robert L. Heath 《Photosynthesis research》1994,39(3):439-451
Tropospheric ozone produced by industrial civilization is widespread. Although the levels are not clearly life threatening, they do have the potential to inhibit normal plant productivity, thought to be by an inhibition of photosynthesis. While the mechanism for this inhibition is not yet clear, there are several hypotheses for its cause. It is unlikely that ozone can penetrate the cell membrane unreacted; therefore, reactions at the plasma membrane either causing general ionic and metabolic disturbance within the cell or causing the production of unidentified toxic products must ultimately produce the alterations within the chloroplasts. While model systems, such as individual biochemicals, isolated chloroplasts, and algae, can give some understanding of possible reactions, they cannot provide the full story. One continuing controversy revolves about the role of stomata in the inhibition process-they play an important role, but the full interaction between stomatal closure and inhibition of photosynthesis has not yet emerged. In order to reach a political compromise on air quality standards, we need to have a good understanding of the fundamental mechanisms by which ozone causes any decline in plant productivity. 相似文献
25.
Growth and maintenance respiration in leaves of northern red oak seedlings and mature trees after 3 years of ozone exposure 总被引:1,自引:0,他引:1
A two-component model of growth and maintenance respiration is used to study the response of northern red oak (Quercus rubra L.) seedlings and 32-year-old trees to sub-ambient (10 μmol h; cumulative dose based on 7 h daily mean), ambient (43 μmol h), and twice-ambient (85 μmolh) ozone. The relative growth rates (RGR) of leaves sampled from seedlings and trees were similar across treatments, as were specific leaf respiration rates (SRR). Growth coefficients estimated from the SRR versus RGR relationship averaged 25-3 mol CO2 kg?1 leaf dry mass produced for seedlings and 21-5 mol kg?1 for trees. Maintenance coefficients ranged from 0-89 to 1-07 mol CO2 kg?1 leaf dry mass d?1 for seedlings and from 0-64 to 0-84 mol kg-1 d?1 for trees. Neither coefficient was affected by ozone. Leaves sampled throughout the growing season also showed little response of respiration to ozone. This occurred despite a 30% reduction in net photosynthesis for trees grown at twice-ambient ozone. These results suggest that growth and maintenance respiration in young northern red oak leaves are not affected by ozone and that in older leaves injury can occur without a parallel increase in so-called ‘maintenance’ respiration. 相似文献
26.
Arundo donax L., commonly known as giant reed, is promising biomass feedstock that is also a notorious invasive plant in freshwater ecosystems around the world. Heretofore, the salt tolerance of A. donax had not been quantified even though anecdotal evidence suggests halophytic qualities. To test whole-plant and leaf level responses, we established a pot experiment on 80 scions propagated from an A. donax population that has naturalized on the shore of the San Francisco Bay Estuary. To quantify growth and physiological responses to salinity (NaCl), A. donax scions were divided into eight treatments and grown for 60 days across a range of salinities (0–42 dS m−1). Classic growth analysis showed >80% reduction in overall growth at the highest salinities. Yet, there was zero mortality indicating that A. donax is able to tolerate high levels of salt. Declining photosynthesis rates were strongly correlated (R2 > 0.97) with decreasing stomatal conductance, which was in turn closely related to increasing salinity. Leaf gas exchange revealed that stomata and leaf limitations of carbon dioxide were three times greater at high salinities. Nonetheless, even when salinities were 38–42 dS m−1 A. donax was able to maintain assimilation rates 7–12 μmol m−2 s−1. Further, by maintaining 50% relative growth at salinities ~12 dS m−1 A. donax can now be classified as ‘moderately salt tolerant’. A. donax leaf gas exchange and whole-plant salt tolerance are greater than many important food crops (i.e. maize, rice), the bioenergy feedstock Miscanthus × giganteus, as well as some uncultivated plant species (i.e. Populus and Salix) that are indigenous in regions A. donax currently invades. The results of this study have implications for both agronomists wishing to expand A. donax to fields dominated by saline soils, and for others who are concerned about the spread of A. donax with altered stream hydrology or sea-level rise. 相似文献
27.
Abstract. Steady-state photosynthesis (Pn ), post-illumination CO2 release rates (R), sucrose-phosphate synthase (SPS) activities, and levels of starch, sucrose and hexoses were measured in the source leaf of corn ( Zea mays L.) during a 16-h photoperiod at 800 μmol m 2 s 1 . Pn and SPS activity remained constant. Carbohydrate pools increased at a linear rate, except the first and last hour of the photoperiod. Both the CO2 evolution rate at the moment of light removal (Rmax ) and SPS activity decreased by one half after the onset of darkness (0 60 min). Sucrose diminished during this period by 40%, whereas the starch remained constant. Thereafter, starch mobilization began, followed by a gradual decline in leaf respiration. The average dark export rate was calculated to be 60% less than that during the day. Maintenance respiration (Rm ) of an attached leaf after 48 h darkness was determined. Plants were illuminated for different intervals (e.g. 5, 10 or 20 min), each followed by dark periods sufficient for respiration to decline to Rm . The ratio of C assimilated in light to that released in dark was 6:1. After the 48-h dark period, the minimal period of illumination (Tmin ) required to restore Pn and Rmax to the original level was determined. A mathematical analysis of the kinetics involved in the recovery of Pn and Rmax provided an estimate of turnover time (0.22h) and pool size 9.15 mmol m 2 ) for the newly fixed carbon. 相似文献
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The green marine macroalga Ulva lactuca L. was found to be able to utilize HCO3? from sea water in two ways. When grown in flowing natural sea water at 16°C under constant dim irradiance, photosynthesis at pH8.4 was suppressed by acetazolamide but unaffected by 4,4′-diisothiocyanostilbene-2,2′-disulphonate. These responses indicate that photosynthetic HCO3? utilization was via extracellular carbonic anhydrase (CA) -mediated dehydration followed by CO2 uptake. The algae were therefore described as being in a ‘CA state’. If treated for more than 10 h in a sea water flow-through system at pH9.8, these thalli became insensitive to acetazolamide but sensitive to 4,4′-diisothiocyanostilbene-2,2′-disulphonate. This suggests the involvement of an anion exchanger (AE) in the direct uptake of HCO3?, and these plants were accordingly described as being in an ‘AE state’. Such thalli showed an approximately 10-fold higher apparent affinity for HCO3? (at pH9.4) than those in the ‘CA state’, while thalli of both states showed a very high apparent affinity for CO2. These results suggest that the two modes of HCO3? utilization constitute two ways in which inorganic carbon may enter the Ulva lactuca cells, with the direct entry of HCO3?, characterizing the ‘AE state’, being inducible and possibly functioning as a complementary uptake system at high external pH values (e.g. under conditions conducive to high photosynthetic rates). Both mechanisms of entry appear to be connected to concentrating CO2 inside the cell, probably via a separate mechanism operating intracellularly. 相似文献
30.
Detached leaves of pea (Pisum sativum) were submitted to water stress at different relative air humidities. The photosynthetic
activity of photosystem 2 (PS2) was monitored by time-resolved picosecond chlorophyll (Chl) fluorescence spectroscopy. In
the first days the well-known fast Chl fluorescence decay was observed which indicated high PS2 activity. After a few days
the average fluorescence decay time τm reached a maximum, depending on the wilting conditions, but always at a relative loss
of leaf mass of 80%. After this maximum, τm decreased within a few hours, the fluorescence decay became similar to that one
of an intact leaf, but an additional fluorescence decay component with a lifetime of 3.6 ns appeared. At first the primary
quinone QA was reduced due to inhibition of the electron transfer to the secondary quinone QB. Simultaneously, water deficiency
caused an electron lack at the oxidizing site of PS2. This disabled the primary electron donor of PS2, tyrosine Z, from reducing
the oxidized reaction centre of PS2 (P680+). Thus a recombination of P680+-pheophytin-QA- took place, and the energy was lost
as heat. With further water stress, QA was decoupled from PS2. The new fluorescence decay component could therefore be assigned
to energetically decoupled antenna complexes.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献