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Jie Wang Rongrong Xue Xueyang Ju Hui Yan Zhou Gao Mohammed Esmail Abdalla Elzaki Lin Hu Rensen Zeng Yuanyuan Song 《Ecology and evolution》2020,10(11):4816-4827
The rice striped stem borer (SSB, Chilo suppressalis) is one of the most destructive pests of rice plants. Si‐mediated rice defense against various pests has been widely reported, and sodium silicate (SS) has been used as an effective source of silicon for application to plants. However, there is quite limited information about the direct effects of Si application on herbivorous insects. SSB larval performance and their insecticide tolerance were examined after they had been reared either on rice plants cultivated in nutrient solution containing 0.5 and 2.0 mM SS or on artificial diets with 0.1% and 0.5% SS. SS amendment in either rice culture medium or artificial diets significantly suppressed the enzymatic activities of acetylcholinesterase, glutathione S‐transferases, and levels of cytochrome P450 protein in the midgut of C. suppressalis larvae. Larvae fed on diets containing SS showed lower insecticide tolerance. Additionally, RNA‐seq analysis showed that SS‐mediated larval insecticide tolerance was closely associated with fatty acid biosynthesis and pyruvate metabolism pathways. Our results suggest that Si not only enhances plant resistance against insect herbivore, but also impairs the insect's capacity to detoxify the insecticides. This should be considered as another important aspect in Si‐mediated plant–insect interaction and may provide a novel approach of pest management. 相似文献
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化感作用研究中的生物测定方法综述 总被引:147,自引:2,他引:145
生物测定是化感作用研究的重要环节。目前很多化感作用研究缺乏规范的生物测定方法。本文对国内外的常用的生物测定方法,这些方法的适应性以及影响生物测定结果的一些因素(如化感物质的收集方法、微生物和溶液渗透压、受体的选择等)进行了综述。并对生物测定结果的表示方法提出了建议。 相似文献
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Govindjee Jan F. H. Snel Oscar J. De Vos Jack J. S. van Rensen 《Physiologia plantarum》1993,89(1):143-148
Depletion of carbon dioxide from cells by formate treatment not only causes a cessation of carbon dioxide fixation, but also a dramatic decrease in the rate of electron transfer between QA , the primary plastoquinone electron acceptor of photosystem II, and the cytochrome b6 /f complex. We show here that this latter phenomenon can be conveniently monitored by the antagonistic effects of light absorbed in photosystems I and II on chlorophyll a fluorescence yield and P700 turnover in intact cells of green algae and cyanobacteria. 相似文献