Purification and properties of cinnamyl alcohol dehydrogenase from higher plants involved in lignin biosynthesis

The purification and characterization of an NADP(H) specific cinnamyl alcohol oxidoreductase is reported. The enzyme, which has been purified 600 ×, shows an absolute specificity for the cinnamyl moiety. The reaction is readily reversible but is strongly inhibited by aldehyde substrates. The enzyme belongs to class A of NAD(P) specific oxidoreductases. The distribution of this activity throughout a wide variety of taxonomically different plant groups as well as plant parts has revealed a possible correlation with lignin biosynthesis.     

Structure,function and metabolism of plant cell wall

The review concerns the newer aspects of plant cell wall construction and modification, including the structure and biosynthesis of basic components during the cell growth and differentiation, as well as their breakdown. The special interest is given to the enzymes incorporated into the cell wall and their specific activity in the biosynthesis and degradation processes, but also in the transfer of glycosyl fragments (blocks), which is connected with its thickening, softening, constructing the channels a.o. New aspects of lignification and specialisation of particular wall fragments, playing various functions, such as fruit ripening, dropping down leaves, fruits and flowers, breaking the dormancy, and others, are also presented.