中国四川菜花露尾甲亚属一新种（鞘翅目：露尾甲科）

记述采自中国四川省的菜花露尾甲属菜花露尾甲亚属1新种:淡翅菜花露尾甲Meligethes(Meligethes)pallidoelytrorum Chen&Kirejtshuk sp.nov.。对其特征作了描述,提供了成虫形态及雌雄外生殖器特征图。新种的主要鉴别特征为:虫体背部体表光滑,鞘翅具稀疏的刻点,跗爪基部具明显的齿。并提出滑菜花露尾甲M.(M.)lutra Solsky,1876和蜜菜花露尾甲M.(M.)melleus Grouvelle,1908是长唇菜花露尾甲M.(M.)vulpes Solsky,1876的新异名。     

年龄增长对GRK5基因缺陷小鼠海马内肿胀轴突丛形成与积累的影响

目的研究G蛋白偶联受体（G protein-coupled receptors）激酶5（GPCR kinase-5,GRK5）基因缺陷和老化交互作用对阿尔茨海默病（Alzheimer＇s disease,AD）早期的病理改变-海马内肿胀轴突丛（swollen axonal clusters,SACs）出现和积累的影响。方法选取5、6、7、9、12～13、18—19月龄雌性GRK5基因敲除小鼠（GRK5 Knockout,GRK5KO）作为观察对象,另选取年龄匹配的雌性野生型（wild type,WT）小鼠为对照,每个年龄段GRK5KO和WT小鼠各4只。用抗人神经原纤维缠结（neurofibrillary tangles,NFTs）特异性抗体的免疫荧光染色方法观察海马内SACs的变化。结果所有小鼠随着年龄增长,海马内SACs逐渐增加;GRKSKO小鼠组海马内NFT^＋ SACs数量较WT型小鼠组显著增加（P〈0．01）;双因素方差分析显示遗传性GRK5基因缺陷和老化双因素对海马内NFT^＋SACs的影响有显著协同效应（P〈0．01）。结论在促进早期AD病理发生的过程中,GRK5缺陷和老化双因素共同加剧了雌性GRKSKO小鼠海马内SACs的形成与积累。     

A large-scale gene discovery for the red palm weevil Rhynchophorus ferrugineus （Coleoptera： Curculionidae）

The red palm weevil （RPW; Rhynchophorusferrugineus） is a devastating pest of palms, prevalent in the Middle East as well as many other regions of the world. Here, we report a large-scale de novo complementary DNA （cDNA） sequencing effort that acquired ~5 million reads and assembled them into 26 765 contigs from 12 libraries made from samples of different RPW developmental stages based on the Roche/454 GS FLX platform. We annotated these contigs based on the publically available known insect genes and the Tribolium castaneum genome assembly. We find that over 80% of coding sequences （CDS） from the RPW contigs have high-identity homologs to known proteins with complete CDS. Gene expression analysis shows that the pupa and larval stages have the highest and lowest expression levels, respectively. In addition, we also identified more than 60 000 single nucleotide polymorphisms and 1 200 simple sequence repeat markers. This study provides the first large-scale eDNA dataset for RPW, a much-needed resource for future molecular studies.     

萨哈林岛舞蛛属一新种记述

记述了采自萨哈林岛1新种,Alopecosa mikhailovi sp.nov.,2♂♂,新种与solivaga种团近似.     

直刺变豆菜叶绿体全基因组及其特征

直刺变豆菜(Sanicula orthacantha)是中国广泛分布的多年生草本植物, 也是一味著名的民族药。本文通过二代高通量测序平台Illumina HiSeq PE150对直刺变豆菜叶绿体全基因组进行测序, 并通过生物信息学方法对其结构特征进行分析。结果表明: 直刺变豆菜叶绿体全基因组大小为157,163 bp, 包括大单拷贝区(large single copy, LSC)、小单拷贝区(small single copy, SSC)和2个反向重复序列(inverted repeat sequence, IRa和IRb), 长度分别为87,547 bp、17,122 bp和26,247 bp, 具有典型被子植物叶绿体基因组环状四分体结构; 共注释得到129个基因, 包括8个核糖体RNA (rRNA)基因、37个转运RNA (tRNA)基因和84个蛋白质编码基因。直刺变豆菜在叶绿体基因组结构、基因种类、排列顺序上与其他伞形科植物基本一致。直刺变豆菜叶绿体全基因组测序的成功为变豆菜属植物完整叶绿体基因组组装及其特征分析提供了新的方法。     

基于Nextflow构建的宏条形码自动化分析流程EPPS

基于宏条形码技术的物种快速检测有助于生物多样性的评估、预测和保护。本文介绍了常用宏条形码分析的步骤和参数设定方法。我们利用Nextflow搭建了一款宏条形码分析流程EPPS, 可以自动化地运行从原始数据的质量控制到环境多样性的比较。Nextflow软件还拥有流程监控的功能, 可视化输出每个进程所消耗的时间与内存。本文还使用测试数据和已发表数据证明该平台能够有效地分析宏条形码数据并可靠地分析环境生物多样性的相似性。     

Integrating Culture-based Antibiotic Resistance Profiles with Whole-genome Sequencing Data for 11,087 Clinical Isolates

Emerging antibiotic resistance is a major global health threat. The analysis of nucleic acid sequences linked to susceptibility phenotypes facilitates the study of genetic antibiotic resistance determinants to inform molecular diagnostics and drug development. We collected genetic data(11,087 newly-sequenced whole genomes) and culture-based resistance profiles(10,991 out of the11,087 isolates comprehensively tested against 22 antibiotics in total) of clinical isolates including18 main species spanning a time period of 30 years. Species and drug specific resistance patterns were observed including increased resistance rates for Acinetobacter baumannii to carbapenems and for Escherichia coli to fluoroquinolones. Species-level pan-genomes were constructed to reflect the genetic repertoire of the respective species, including conserved essential genes and known resistance factors. Integrating phenotypes and genotypes through species-level pan-genomes allowed to infer gene–drug resistance associations using statistical testing. The isolate collection and the analysis results have been integrated into GEAR-base, a resource available for academic research use free of charge at https://gear-base.com.     

Patented Biologically-inspired Technological Innovations： A Twenty Year View

1 Introduction Biomimetics or bionics is having an increasing role in innovation[1]. It offers the promise of novel routes to technological innovation; a recent paper highlighted the potential of studies of biological systems to initiate patents in the engineering sciences and technology[2]. Media coverage, particularly in business periodicals is increasing, for example, The Economist recently re- ported on the growth of biomimetics and its impact on technology[3]. There are two principal rou…     

中国缺叉多距石蛾属六新种记述(毛翅目,多距石蛾科)

研究了中国毛翅目多距石蛾科缺叉多距石蛾属种类,并报道了其中亲缘关系较近的6新种,即端截缺叉多距石蛾Polyplectropus truncatufus sp.nov.,端突缺叉多距石蛾Polyplectropus convexus sp.nov.,斧端缺叉多距石蛾Polyplectropus dolabriformis sp.nov.,颚状缺叉多距石蛾Polyplectropus mandibuliformis sp.nov.,距枝缺叉多距石蛾Polyptectropus rectangulus sp.nov.,喙端缺叉多距石蛾Polyplectropus rostriformis sp.nov..新种模式标本保存在南京农业大学昆虫标本馆.