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101.
The basal cut end of coppice shoot cuttings of Pongamia pinnata was treated for 24 h with 0 (water treated control) or 5.0 mmol/L of KMnO4, KCI, and KH2PO4 or 2.5 mmol/L of K2HPO4 and K2SO4. Inorganic salts of P, S, Cl and Mn significantly influenced IAA ionization and adventitious rhizogenesis. P and S salts had lower IAA ionization potential, but more pronounced effect on adventitious rhizogenesis than Cl and Mn salts. The linear regression analysis also established negative correlations between salt induced IAA ionization with various characteristics of adventitious rhizogenesis such as sprouting (r = -0.83, p < 0.05), rooting (r = -0.82, p < 0.05), root number (r = -0.95, p < 0.01), and root length (r = -0.80, p < 0.1). The implication of IAA ionization in adventitious rhizogenesis has been discussed and the possible role of inorganic salts therein suggested. 相似文献
102.
103.
William J. Page 《Biometals》1995,8(1):30-36
The addition of manganese oxides to iron-limited medium promoted the formation of the pyoverdin siderophore azotobactin by Azotobacter vinelandii. When active-MnO2 was used, there was greatly decreased iron uptake into the cells, hyperproduction of azotobactin and the abiotic, chemical destruction or adsorbtion of the catechol siderophores azotochelin and aminochelin by this strong oxidizing agent. Although the iron content of the cells was the same as iron-limited cells, the growth of cells in medium with active-MnO2 was increased 1.5- to 2.5-fold over iron-limited controls. This growth promotion was not caused by iron contaminating the oxide or by manganese solubilized from the oxide. Soluble 0.05–4 mm Mn2+ inhibited the growth of iron-limited cells and had a minimal effect on iron uptake, but caused hyperproduction of azotobactin. This later effect was caused by the inhibition of soluble ferric reductase, in a manner identical to that previously observed for Zn2+. These results suggest that active-MnO2 may interfere with a surface-localized iron uptake site, possibly another ferric reductase. The reason for the growth promotion by active-MnO2 remains unknown, but is most likely related to decreased oxygen toxicity. 相似文献
104.
Free-radical polymerization of acrylamide by manganese peroxidase produced by the white-rot basidiomycete Bjerkandera adusta 总被引:1,自引:0,他引:1
Kenji Iwahara Mio Hirata Yoichi Honda Takashi Watanabe Masaaki Kuwahara 《Biotechnology letters》2000,22(17):1355-1361
Acrylamide was polymerized to give polyacrylamide using manganese peroxidase (MnP) produced by the basidiomycete Bjerkandera adusta. The molecular weight of the polymer synthesized by MnP was 155000, higher than those obtained with other reaction systems using horseradish peroxidase and a redox initiator. The 13C-NMR spectrum showed that polyacrylamide was atactic. Electron spin resonance analysis revealed that 2,4-pentanedione added as an initiator was first oxidized to generate a carbon-centered radical, which initiated radical additive polymerization of acrylamide. 相似文献
105.
The aim of this study was to assess the effects of cold-restraint stress on blood and the other tissue levels of trace elements.
The experiment was performed on male Swiss albino rats. The animals were divided to two groups: control and stressed groups.
In the stressed group, the rats were subjected to immobilization for 4 h at 4°C. At the end of the experimental period, blood
and tissue samples were collected from all of the animals. The levels of zinc, copper, magnesium, and manganese were measured
using flame and graphite furnace atomic absorption spectrometry. In rats subjected to stress, the zinc levels of serum were
increased, whereas in the duodenum and brain, zinc was found to decrease when compared to the control. The magnesium content
of both the stomach and duodenum were reduced, but the manganese levels of the liver, kidneys, and brain significantly increased
because of stress. In conclusion, cold-restraint stress may affect the metabolic process by changing the distributions of
zinc, magnesium, and manganese in tissues. 相似文献
106.
Shinichi Yoshida Saori Yonehara Shigemi Minami Hyo-cheol Ha Kenji Iwahara Takashi Watanabe Yoichi Honda Masaaki Kuwahara 《Mycoscience》1996,37(4):417-425
Manganese peroxidase (MnP) and lignin peroxidase (LiP) were produced by growing a white-rot fungusBjerkandera adusta statically, on a wood meal/wheat bran culture in flasks. MnP and LiP reached their maximum activity after 6 and 19 days of
inoculation, respectively. Both MnP and LiP are thought to be important enzymes in lignin biodegradation byB. adusta. Ion exchange chromatography showed thatB. adusta produced a single LiP and a single MnP enzyme in wood meal/wheat bran culture. These enzymes were separated and characterized.
The molecular weight of MnP was 46,500 with a pl of 3.9. The molecular weight of LiP was estimated to be 47,000 with a pl of 3.5. Spectral analysis demonstrated that both enzymes are heme proteins. Production of these enzymes was also achieved
using a rotarysolid culture fermenter. MnP, LiP and veratryl alcohol oxidase were produced byB. adusta in the fermenter. 相似文献
107.
Josiane Arnaud Pierre Bourlard Bernard Denis Alain E. Favier 《Biological trace element research》1996,53(1-3):129-136
This study was carried out to assess manganese (Mn) status after an acute episode of myocardial infarction. Plasma and erythrocyte
Mn concentrations were measured from admission to hospital to day 15 postadmission in 21 patients suffering from acute myocardial
infarction and in three control groups. The determination of Mn in these biological fluids was performed by electrothermal
atomic absorption spectrometry. Plasma Mn was higher (p<0.01) and erythrocyte Mn was similar in the acute myocardial infarction group compared to healthy age-matched control group.
Plasma and erythrocyte Mn remained unchanged during the 2 wk after acute myocardial infarction and were not correlated to
enzyme activities. A decrease of erythrocyte Mn with age, expressed in nmol/L, was noted (p<0.02). These results suggest that plasma and erythrocyte Mn do not provide an indication of myocardial damage. Nonetheless,
Mn status in elderly merits further attention. 相似文献
108.
Manganese supplementation resulted in higher polysaccharide levels and reduced cellular pigmentation by more than 8- or 17-fold after growth for 7d of the fungus Aureobasidium pullulans ATCC 42023 on sucrose or corn syrup, respectively, as a carbon source. The melanin content of the polysaccharide elaborated by ATCC 42023 cells also decreased if MnCl2 was added to the medium. The pullulan content of the polysaccharide synthesized by ATCC 42023 on sucrose was found to increase with increasing levels of manganese, whereas it was lower during growth on corn syrup if manganese was present. 相似文献
109.
The IRT1 protein from Arabidopsis thaliana is a metal transporter with a broad substrate range 总被引:25,自引:0,他引:25
Korshunova Yulia O. Eide David Gregg Clark W. Lou Guerinot Mary Pakrasi Himadri B. 《Plant molecular biology》1999,40(1):37-44
The molecular basis for the transport of manganese across membranes in plant cells is poorly understood. We have found that IRT1, an Arabidopsis thaliana metal ion transporter, can complement a mutant Saccharomyces cerevisiae strain defective in high-affinity manganese uptake (smf1). The IRT1 protein has previously been identified as an iron transporter. The current studies demonstrated that IRT1, when expressed in yeast, can transport manganese as well. This manganese uptake activity was inhibited by cadmium, iron(II) and zinc, suggesting that IRT1 can transport these metals. The IRT1 cDNA also complements a zinc uptake-deficient yeast mutant strain (zrt1zrt2), and IRT1-dependent zinc transport in yeast cells is inhibited by cadmium, copper, cobalt and iron(III). However, IRT1 did not complement a copper uptake-deficient yeast mutant (ctr1), implying that this transporter is not involved in the uptake of copper in plant cells. The expression of IRT1 is enhanced in A. thaliana plants grown under iron deficiency. Under these conditions, there were increased levels of root-associated manganese, zinc and cobalt, suggesting that, in addition to iron, IRT1 mediates uptake of these metals into plant cells. Taken together, these data indicate that the IRT1 protein is a broad-range metal ion transporter in plants. 相似文献
110.
Experiments were conducted over two years with Lupinus angustifolius L. on a site with acid sandy soil near Esperance, Western
Australia to determine if deep placed manganese fertilizer increases lupin grain yield. Manganese at 4 and 8 kg ha−1 was placed below the surface immediately before sowing at 4, 20 and 30 cm and 4, 8, 12, 16 and 20 cm in 1987 and 1988 respectively.
Foliar Mn applied at 1 kg ha−1 when the first order laterals were in mid-flowering stage, was also compared. Increasing the depth of Mn placement increased
grain yield in both years. The deepest placed Mn increased grain yields by 255 kg ha−1 (10%) and 430 kg ha−1 (106%) in year 1 and year 2 over the shallow (4 cm) placed Mn. The higher responses to deep placed Mn occurred in year 2,
the year with the driest spring and most intense aphid infestations. Foliar applied Mn was as effective as most deep placed
Mn treatments, except for the highest rate (8 kg ha−1) at the greatest depth (20 cm) in year 2. The higher rate of applied Mn gave the best grain yields.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献