排序方式: 共有38条查询结果,搜索用时 62 毫秒
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电诱导细胞融合仪的研制及在真菌原生质体融合中的应用 总被引:3,自引:0,他引:3
细胞融合仪是进行细胞融合研究的核心装置。作者研制出一种性能参数较好而造价低廉的电诱导细胞融合仪。其输出交流电场频率及电压峰峰值分别为0.5-4.0MHz和0-70V;脉冲时程和幅度分别为4-100μs和0-500V;带负载能力大于几种国内外产品。用本装置对香菇和丝核菌原生质体进行了融合诱导,对电场诸参数与融合效果之间的关系进行了探讨。结果表明本装置性能稳定,使用效果不次于进口日本产品,可用于真菌、细菌或植物的细胞融合研究。 相似文献
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谈细胞生物学启发讨论式教学中的问题设置 总被引:2,自引:0,他引:2
启发讨论式教学是素质教育对各科教学的新要求。问题设置是启发讨论式教学中的关键环节。探讨了细胞生物学启发讨论式教学中问题设置的基本方式及其应注意的几个方面。 相似文献
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A flexible method is proposed for group sequentially performed clinical trials which allows for an adaptive, data‐driven sample size reassessment at each stage. By also adaptively assigning different weights to the several stages the total number of study parts can be steered to an intended early or late end of the trial in dependence on all information available prior to a stage. Although at each stage the null hypothesis is tested on rejection, the full level‐α‐test is preserved at the end of the study. The proposed method is not restricted to normally distributed responses. The discussed adaptive designing is a useful tool provided that a priori information about parameters involved in the trial are not available or subject to uncertainty. The presented learning algorithm enables the complete self‐designing of a study. 相似文献
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染色体重组与连锁互换是遗传学教学的重点和难点。为使学生更好地理解此方面知识, 我们课题组前期利用图位克隆(map-based cloning)技术, 克隆了1个调控水稻(Oryza sativa)类病变表型的基因SPL5, 并基于此设计了一个新的综合型遗传学实验, 即利用DNA分子标记对基因进行定位。实验中学生利用水稻spl5突变体与野生型杂交获得的F2代定位群体和多态性分子标记, 对spl5突变进行染色体连锁分析、初步定位和遗传作图。该教学实验不仅可有效促进学生对遗传学三大定律的理解, 而且对其开阔视野、提高解决问题和团队协作的能力也有促进作用。 相似文献
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G. Herrendrfer 《Biometrical journal. Biometrische Zeitschrift》1979,21(1):11-16
The methods of optimal designing of experiments proposed by WALD (1943) are used for determination of an Aσ2-optimal concrete design for estimation of σ2′ = (σ, σ) in case of one-way analysis of variance. Starting point of definition of the optimality criterion is a quadratic loss matrix. 相似文献
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《Journal of biological education》2012,46(2):88-98
Protoplasmic streaming (cyclosis) in plant cells has long been presented as a purely qualitative exercise in microscopy. Using simple formulae, it is possible to determine the energetics of this process (in terms of ATP hydrolysis) and its relation to the total energy expenditure of the plant cell. This exercise can provide a valuable integration of physicochemical principles and microscopical observation. 相似文献
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In spite of genome sequences of both human and N. gonorrhoeae in hand, vaccine for gonorrhea is yet not available. Due to availability of several host and pathogen genomes and numerous tools for in silico prediction of effective B-cell and T-cell epitopes; recent trend of vaccine designing has been shifted to peptide or epitope based vaccines that are more specific, safe, and easy to produce. In order to design and develop such a peptide vaccine against the pathogen, we adopted a novel computational approache based on sequence, structure, QSAR, and simulation methods along with fold level analysis to predict potential antigenic B-cell epitope derived T-cell epitopes from four vaccine targets of N. gonorrhoeae previously identified by us [Barh and Kumar (2009) In Silico Biology 9, 1-7]. Four epitopes, one from each protein, have been designed in such a way that each epitope is highly likely to bind maximum number of HLA molecules (comprising of both the MHC-I and II) and interacts with most frequent HLA alleles (A*0201, A*0204, B*2705, DRB1*0101, and DRB1*0401) in human population. Therefore our selected epitopes are highly potential to induce both the B-cell and T-cell mediated immune responses. Of course, these selected epitopes require further experimental validation. 相似文献
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Subhojyoti Chatterjee Akshaya Lakshmi Narasimhaiah Sanjay Kundu Santosh Anand 《Bioinformation》2012,8(24):1195-1201
Diabetes is a metabolic disorder associated with either improper functioning of the beta-cells or wherein cells fail to use insulin
properly. Insulin, the principal hormone regulates uptake of glucose from the blood into most of the cells except central nervous
system. Therefore, deficiency of insulin or the insensitivity of its receptors plays a key role in all forms of diabetes. In the present
work, attempt has been made to find out plant sources which show anti hyperglycaemic activity (AhG) (i.e. compounds that bring
down the blood glucose level in the body). Ayurvedic plants showing AhG activity formed the basis of our study by using the platform
of Computer Aided Drug Designing (CADD). Among 600 plants showing AhG activity, 500 compounds were selected and
screened, out of which 243 compounds showed drug likeness property that can be used as therapeutic ligand/drug. Initial
screening of such compounds was done based on their drug likeness or biochemical properties. Dynamic interaction of these
molecules was captured through Protein-Ligand study. It also gave an insight of the binding pockets involved. Bench marking of
all the parameters were done using the diabetic inhibitor drug, Glipizide. Pharmacokinetic studies of the compounds such as
Aloins, Capparisine, Funiculosin and Rhein exhibited less toxicity on various levels of the body. As a conclusion these ligands can
lay a foundation for a better anti-diabetic therapy.