支原体培养基的改良及其效果评价

为了改良支原体培养基配方,评价其效果。按《中华人民共和国药典》(以下简称药典)中规定的灵敏度比较方法,将实验室制备的新型支原体改良培养基与《药典》中支原体检查法推荐的处方培养基和商品化支原体培养基进行灵敏度效果比较试验。结果表明,改良后的支原体肉汤和半流体培养基,与接种口腔支原体和肺炎支原体及其它支原体的精氨酸培养基、支原体肉汤培养基无显著差异;与接种肺炎支原体的支原体半流体培养基无显著差异;与接种口腔支原体的支原体半流体培养基差异显著。因此经改良后的支原体培养基灵敏度能够满足药典规定的要求,但其操作简便,且成本低于现有的支原体培养基。     

RAPD标记与作物改良

建立于ＤＮＡ基础之上的分子标记对于作物改良和多态性研究具有重要作用。     

水稻中的无融合生殖研究及问题

本文综述了植物无融合生殖概念的发展历史。简述了目前报道的裟上主要无融合生殖材料的特点特征以及近年来的一些新进展。同时指出了水稻无融合生殖研究中存在的问题和注意事项。     

Backpropagation Neural Network for Processing of Missing Data in Breast Cancer Detection

BackgroundA complete dataset is essential for biomedical implementation. Due to the limitation of objective or subjective factors, missing data often occurs, which exerts uncertainty in the subsequent data processing. Commonly used methods of interpolation are interpolating substitute values that keep minimum error. Some applications of statistics are usually used for handling this problem.MethodsWe are trying to find a higher performance interpolation method compared with the usual statistic methods, by using artificial intelligence which is in full swing today. The prediction and classification of backpropagation neural network are used in this paper, describes a missing data interpolation method to propose the interpolation model that mines association rules in the data. In the experiment, depending on a multi-layer network structure, the model is trained and tested by sample data, constantly revises network weights and thresholds. The error function decreases along the negative gradient direction and approaches the expected real output. The model is validated on the breast cancer dataset, and we select real samples from the data set for validation, moreover, add four traditional methods as a control group.ResultsThe proposed method has great performance improvement in the interpolation of missing data. Experimental results show that the interpolation accuracy of our proposed method (84%) is higher than four traditional methods (1.33%, 74.67%, 73.33%, 77.33%) as mentioned in this paper, BPNN stays low in MSE evaluation. Finally, we analyze the performance of various methods in processing missing data.ConclusionsThe study in this paper has estimated missing data with high accuracy as much as possible to reduce the negative impact in the diagnosis of real life. At the same time, it can also assist in missing data processing in the biomedical field.     

水稻耐盐碱鉴定标准评价及建议与展望

对国内外水稻耐盐碱鉴定评价标准进行了评述,指出目前采用的评价标准存在着对盐碱害判断不够全面、准确的问题,只适用对水稻进行生物耐盐力的鉴定,而不适合对水稻的农业耐盐力进行鉴定;提出了对水稻耐盐碱鉴定的标准品种、方法、鉴定胁迫浓度等进行改进的建议,强调对水稻进行全生育期的耐盐碱性鉴定。     

A new type of orally active anti-diabetic Zn(II)-dithiocarbamate complex

In order to find orally active Zn(II) complexes that can treat diabetes mellitus (DM) at low doses, four new Zn(II)-dithiocarbamate complexes with Zn(II)-sulfur coordination bonds were prepared and their in vitro insulinomimetic activity and in vivo anti-diabetic ability were evaluated. Among the Zn(II)-dithiocarbamate complexes, the bis(pyrrolidine-N-dithiocarbamate)zinc(II) (Zn(pdc)(2)) complex was found to be the most effective in terms of inhibiting free fatty acid-release and enhancing glucose-uptake in adipocytes. After oral administration of the Zn(pdc)(2) complex to KK-A(y) mice with obesity and type 2 DM, we observed that the high blood glucose levels in the mice were lowered from approximately 500 mg/dL to 350 mg/dL within 6 days, and the effect was maintained during the administration period. Also, indicators of insulin resistance such as serum insulin, leptin, and triglyceride levels were also reduced compared with those in untreated mice. Moreover, the Zn(pdc)(2) complex improved not only the hypertension in the mice, but also the adiponectin level in the serum. On the basis of the results, the Zn(pdc)(2) complex is proposed to improve hyperglycemia and insulin resistance in type 2 DM animals on daily oral administrations.     

以玻璃酸钠为载体改进利福平滴眼液的配制方法

目的:改进利福平滴眼液的配制方法。方法:以计算量的盐酸溶解利福平,然后用等摩尔量的氢氧化钠中和,生成等处方量的氯化钠;并以玻璃酸钠为载体,配制利福平滴眼液。结果:该滴眼液质量稳定、可控,对眼睛无刺激性。结论:本办法设计巧妙,切实可行,既克服了的利福平的溶解困难,又解决了用乙醇作溶剂所产生的眼睛刺激性问题。     

作物抗旱的作用机制及其基因工程改良研究进展

以不同靶标基因为例,分不同作用机制（如渗透调节、清除氧自由基、LEA蛋白、水通道蛋白、脱水素和气孔关闭等）,简要介绍了近10年来人们利用转基因技术改良作物抗旱性的研究进展,以期为我国农业的抗旱性研究提供一些思路。     

改善植物大规模组织培养条件的研究进展

植物组织培养技术具有巨大的应用价值,传统组织培养存在许多缺点,许多学者从培养基、培养容器、环境条件(光照、气体等)进行了研究和改善,取得了一定的成效。综述了这些方面的研究进展。     

对483 例住院病人退院现象的调查分析

目的：分析住院病人退院现象的增多,找出医院管理环节中诸多不合理的因素,合理调配医院现有资源,加强医院整体管理水平,减少不正常退院现象的发生。