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61.
乳糖对产木聚糖酶基因工程菌1020的诱导作用及碳氮源优化 总被引:6,自引:1,他引:5
研究了国产及进口LB培养基对产嗜热性木聚糖酶基因工程菌1020生长及产酶的影响,并以国产原料为基准,优化了培养基。结果表明,国产的酵母膏及蛋白胨和进口原料相比,缺少某些成分,单纯增加用量不能弥补这种差别;乳糖可以代替昂贵的IPTG起到有效的诱导作用;10g·L-1的乳糖可兼起能源及诱导剂的双重效果,菌的生物量和产酶量达到最佳;发酵8h后补加5g·L-1乳糖,生物量及酶活皆有提高。复合氮源优于单一的无机或有机氮源。优化后的酶活从进口LB培养基的270U~290U提高到1700U~1800U。 相似文献
62.
利用农业废弃物生产嗜热真菌(T.1anuginosus)耐热木聚糖酶的固体发酵研究 总被引:5,自引:1,他引:4
研究了嗜热真菌(Thermomyces lanuginosus CBS288.54-M18)生产木聚糖酶的碳氮源组成、料水比、培养基初始pH值、发酵温度及接种量等的影响。结果表明,以麦麸和玉米芯粉(8:2)作为复合碳源,酵母膏和胰蛋白胨(1:1)作为复合氮源时,菌株所产的木聚糖酶量相比未优化碳氮源的培养基提高幅度高达200%。其最佳产酶的料水比为1:3,培养基初始pH7.0为最适。菌株在50℃条件下发酵5d,能够得到活力高达15023U/g干基碳源的木聚糖酶制剂,且该酶制剂不合纤维素酶和蛋白酶活性。 相似文献
63.
青霉菌m8产胞外木聚糖酶的纯化及其性质研究 总被引:5,自引:0,他引:5
青霉菌m8产胞外木聚糖酶的适合培养基 (g/L) :含麦草粉 4 0 ,(NH4) 2 SO44.5 ,KH2 PO41.0 ,MgSO4·7H2 O 0 .5 ,NaCl 0 .3,Tween80 3.0 ,CaCO3 1.0。培养物中该酶经过离子交换和分子筛层析两步处理 ,粗酶被浓缩了 31倍 ,比活力达 4 6 7,收率为 5 0 %。该酶的最适 pH值为 4 .5 ,最适反应温度为 5 5℃ ,可被K+ ,Ca2 + ,Mg2 + 离子激活 ,而被Ag+ ,Fe3 + 和Cu2 + 离子纯化 ,其Km值为 4 .8× 10 -2 g/L。 相似文献
64.
利用真菌纤维素结合域(CBD)保守性序列进行草菇木聚糖酶cDNA的克隆 总被引:3,自引:0,他引:3
木聚糖是植物细胞壁的主要组分,它是木糖以β1 ,4 木糖苷键形成主链,乙酰基,阿拉伯糖基等为附链组成的复合多聚糖.木聚糖酶可以降解木聚糖主链,在木聚糖的生物降解中起着非常重要的作用[1 ] .根据木聚糖酶催化域(catalyticdomain ,CD)氨基酸序列的相似性,木聚糖酶可分为两个家 相似文献
65.
Xylanase-induced reduction of chlorine dioxide consumption during elemental chlorine-free bleaching of different pulp types 总被引:4,自引:0,他引:4
Andreas M. Madlala Sadhvir Bissoon Suren Singh Lew Christov 《Biotechnology letters》2001,23(5):345-351
The potential of crude xylanase from Thermomyces lanuginosus and Xylanase P (a commercial xylanase) was evaluated in bleaching of various paper pulp types. Xylanases released chromophores and reducing sugars and decreased kappa number of pulps. Chlorine-bleached, alkali-extracted bagasse and post-oxygen kraft pulps, pretreated with enzymes, gained over 5 brightness points over controls. Biobleaching of soda-aq pulp with Xylanase P produced chlorine dioxide savings of up to 30% or 4.5 kg chlorine dioxide t–1 pulp. 相似文献
66.
Robert F.H. Dekker Ana-Flora D. Vasconcelos Aneli M. Barbosa Ellen C. Giese Luzia Paccola-Meirelles 《Biotechnology letters》2001,23(24):1987-1993
A new physiological role for veratryl alcohol in fungi important in the biodegradation of the lignified plant cell wall is presented. Botryosphaeria sp., grown on starch, pectin, cellulose or xylan produced amylase, pectinase, cellulase, xylanase and laccase, whereas glucose and xylose repressed the synthesis of cellulase and xylanase, but not laccase. When cultured on each of these substrates in the presence of veratryl alcohol, laccase activity increased but the activities of amylase, pectinase, cellulase and xylanase significantly decreased. Basal medium containing softwood kraft lignin in the presence of veratryl alcohol induced laccases above constitutive levels. Ethyl alcohol also stimulated laccase production. 相似文献
67.
研究了里氏木霉GXC产木聚糖酶的条件和酶学性质。结果表明,适宜产酶碳源为乳糖、甘露糖、棉子糖、木聚糖和麸皮,氮源为牛肉膏和酵母膏;产酶的最适初始pH为4.0,30℃培养60h。对以麸皮为碳源的培养液进行纯化的酶特性研究表明,木聚糖酶的最适反应温度为50℃,pH为5.5,该酶在pH5.0(7.0和40℃以下相对稳定。Fe3+和Mn2+对木聚糖酶有较大的促进作用,Cu~2+、Fe~2+和Ca~2+ 具有抑制作用。 相似文献
68.
Sawao Murao Toyokazu Nishino Yasutaro Hamagishi 《Bioscience, biotechnology, and biochemistry》2013,77(4):887-889
An α-glucosidase was purified from sweet corn seeds by fractionation with ammonium sulfate, chromatographies on CM-Sepharose and Sepharose 4B, and gel filtrations on Sephadex G-100. The enzyme was homogeneous in disc electrophoretic analysis. The molecular weight was estimated to be about 9.6 × 104 by SDS-disc electrophoresis.The enzyme showed high activities toward maltose, nigerose, phenyl-α-maltoside, and maltooligosaccharides. The ratios of maximum velocity for maltose, nigerose, kojibiose, isomaltose, phenyl-α-glucoside, phenyl-α-maltoside, panose, turanose, and soluble starch were estimated to be 100 : 78 : 17 : 11 : 28 : 100 : 31 : 3.4 : 126, and the Km values for these substrates, 1.5 mM, 1.4 mM, 0.48 mM, 14 mM, 4.2 mM, 1.1 mM, 5.0 mM, 0.28 mM and 52mg/ml, respectively. The maximum velocity for soluble starch was high, but this α-glucan was not a favorable substrate because the Km value was also very high. The Vmax for maltooligosaccharides were somewhat dependent on the degree of polymerization (n). The Km values for substrates having four or more glucose units increased with the increase in n. 相似文献
69.
Kota Nakatani Yuta Katano Kenji Kojima Teisuke Takita Rie Yatsunami Satoshi Nakamura 《Bioscience, biotechnology, and biochemistry》2018,82(10):1715-1723
Site saturation mutagenesis library is a recently developed technique, in which any one out of all amino acid residues in a target region is substituted into other 19 amino acid residues. In this study, we used this technique to increase the thermostability of a GH10 xylanase, XynR, from Bacillus sp. strain TAR-1. We hypothesized that the substrate binding region of XynR is flexible, and that the thermostability of XynR will increase if the flexibility of the substrate binding region is decreased without impairing the substrate binding ability. Site saturation mutagenesis libraries of amino acid residues Tyr43–Lys115 and Ala300–Asn325 of XynR were constructed. By screening 480 clones, S92E was selected as the most thermostable one, exhibiting the residual activity of 80% after heat treatment at 80°C for 15 min in the hydrolysis of Remazol Brilliant Blue-xylan. Our results suggest that this strategy is effective for stabilization of GH10 xylanase.
Abbreviations: DNS: 3,5-dinitrosalicylic acid; RBB-xylan: Remazol Brilliant Blue-xylan 相似文献
70.
A xylanase was purified from a commercial crude xylanase, Pulpzyme HC, and used for the bleaching of kraft pulp in the absence
or in the presence of nonionic surfactants, Tween 20, Tween 80, and Igepal C930. The purified xylanase has a molecular weight
of 23,500 as determined by a reducing SDS-PAGE. Tween 20 was most effective to enhance the efficiency of the enzymatic bleaching
of kraft pulp by xylanase. 相似文献