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Fluorescence induction curves in chloroplasts phosphorylated by the thylakoid protein kinase activated at low light intensity and high chlorophyll concentration have been measured. At 5 mM Mg2+, phosphorylation did not preferentially quench variable fluorescence. At 1 mM, preferential quenching of variable fluorescence was observed, indicating a second effect of phosphorylation at low Mg2+ (Horton, P. and Black, M.T. (1982) Biochim. Biophys. Acta 680, 22–27). Comparison of the extent of fluorescence decrease and the resulting ratio of variable to maximum fluorescence after phosphorylation and after lowering Mg2+ concentration demonstrated a difference between these two mechanisms of lowering of fluorescence. The significance of these results in terms of how phosphorylation may alter membrane organization is discussed. 相似文献
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应用同位素放射自显影技术,以~3H-尿嘧啶和~3H-亮氨酸作前体,对人工萌发花粉中新合成的 RNA 和蛋白质进行标记;并借助 RNA 合成抑制剂放线菌素 D 和蛋白质合成抑制剂环己亚胺的对比试验,确证了君子兰花粉在萌发过程中,向外释放了新合成的 RNA 和蛋白质。动态如下:标记后1小时,花粉尚未萌发,大量的 RNA 和蛋白质已合成;2小时后,新合成的蛋白质向花粉管方向转移;5小时后扩散到培养基中。新合成的 RNA 在90分钟内暂留在营养核和生殖细胞中;2小时后扩散到花粉细胞质;3小时后集中到花粉管顶端;5小时后释放到花粉外。文章还讨论了技术的关键问题和现象的生物学意义。 相似文献
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Hillary M. Masundire 《Hydrobiologia》1992,232(1):11-17
Limnological measurements during the first year of filling of the newly formed Mazvikadei Reservoir in Zimbabwe showed variations
in dissolved oxygen as well as soluble forms of phosphorus and nitrogen. The ratios of calcium to other major cations were:
Ca:Mg = 0.38, Ca:Na = 0.50 and Ca:K = 2.0. The low Ca:Mg ratio may suppress development of mollusc populations, thus keeping
the reservoir snail free. Conductivity was 160 μScm-1. Daytime pH was 6.95–7.8. The water was dark-brown in colour, 90% of incident light was attenuated in the top 0.75 m in May,
and in the top 3.5 m in August. Positive net photosynthesis was only recorded above a depth of 0.1 m, at 0.5 m and 1.0 m depths,
it was negative. Zooplankton consisted of nine rotifer, four cladoceran, two cyclopoid and one calanoid species. 相似文献
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2008年5月在崇明岛西端潮间带水域进行鱼类调查时,发现一尾长江河口及上海地区的鱼类新记录种——长身鳜,隶属鲈形目鮨科。主要特征如下:体较细长,近似圆筒形;头长,稍平扁。吻尖。口裂大,下颌明显突出;上颌达眼下方。前鳃盖骨后缘有10枚细锯齿,腹缘有向前倾斜的鳃盖骨棘2枚。鳃盖后端有1枚扁平棘,其上端有较小的短棘1枚。体黄褐色,头背和体侧具不规则的黑点和黑斑。近数十年来由于过度捕捞和水环境污染等原因,导致其资源量急剧减少,已被我国列入濒危动物红皮书。目前,关于长身鳜的资源状况研究十分薄弱,尤其是其繁殖习性、食性和发育等生物学特征研究还未见报道。因此,亟待开展此方面的研究和保护工作。 相似文献
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Martins HR Toledo MJ Veloso VM Carneiro CM Machado-Coelho GL Tafuri WL Bahia MT Valadares HM Macedo AM Lana M 《Experimental parasitology》2006,112(4):237-246
Herein, we have analyzed major biological properties following dual-clone Trypanosoma cruzi infections in BALB/c mice. Eight T. cruzi clonal stocks, two of each principal genotype, including genotype 19 and 20 (T. cruzi I), hybrid genotype 39 (T. cruzi) and 32 (T. cruzi II) were combined into 24 different dual-clone infections. Special attention was given to characterize biological parameters assayed including: prepatent period, patent period, maximum of parasitemia, day of maximum parasitemia, area under the parasitemia curve, infectivity, mortality, and hemoculture positivity. Our findings clearly demonstrated that features resultant of dual-clone infections of T. cruzi clonal stocks did not display either the characteristics of the corresponding monoclonal infections or the theoretical mixture based on the respective monoclonal infections. Significant changes in the expected values were observed in 4.2-79.2% of the mixtures considering the eight biological parameters studied. A lower frequency of significant differences was found for mixtures composed by phylogenetically distant clonal stocks. Altogether, our data support our hypothesis that mixed T. cruzi infections have a great impact on the biological properties of the parasite in the host and re-emphasizes the importance of considering the possible occurrence of natural mixed infections in humans and their consequences on the biological aspects of ongoing Chagas' disease. 相似文献