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51.
Expressed rhodopsins were detected by proteomic analysis in an investigation of potential signal receptors in the cell membrane of the marine heterotrophic dinoflagellate Oxyrrhis marina (CCMP604). We inferred these to be sensory rhodopsins, a type of G-protein-coupled receptor trans-membrane signaling molecule. Because phototactic behavior based on sensory rhodopsins has been reported in other protists, we investigated the photosensory response of O. marina. This dinoflagellate exhibited strongest positive phototaxis at low levels (2-3 μE/m(2)/s) of white light when the cells were previously light adapted and well fed. Positive phototaxis was also found for blue (450 nm), green (525 nm), and red (680 nm) wavelengths. In a further test, O. marina showed significantly greater phototaxis toward concentrated algal food illuminated by blue light to stimulate red chlorophyll-a autofluorescence in the prey, compared with using bleached algae as prey. Concentration of a cytoplasmic downstream messenger molecule, cyclic adenosine monophosphate, a component of the signaling pathway of G-protein-coupled receptor molecules, rapidly increased in O. marina cells after exposure to white light. In addition, treatment with hydroxylamine, a rhodopsin signaling inhibitor, significantly decreased their phototactic response. Our results demonstrate that a heterotrophic marine dinoflagellate can orient to light based on rhodopsins present in the outer cell membrane and may be able to use photosensory response to detect algal prey based on chlorophyll autofluorescence. 相似文献
52.
A modified fluorescence in situ hybridization (FISH) method was used to analyze bacterial prey composition in protistan food vacuoles in both laboratory and natural populations. Under laboratory conditions, we exposed two bacterial strains (affiliated with beta- and gamma-Proteobacteria -- Aeromonas hydrophila and Pseudomonas fluorescens, respectively) to grazing by three protists: the flagellates Bodo saltans and Goniomonas sp., and the ciliate Cyclidium glaucoma. Both flagellate species preferably ingested A. hydrophila over P. fluorescens, while C. glaucoma showed no clear preferences. Differences were found in the digestion of bacterial prey with B. saltans digesting significantly faster P. fluorescens compared to two other protists. The field study was conducted in a reservoir as part of a larger experiment. We monitored changes in the bacterial prey composition available compared to the bacteria ingested in flagellate food vacuoles. Bacteria detected by probe HGC69a (Actinobacteria) and R-BT065 were negatively selected by flagellates. Bacteria detected by probe CF319a were initially positively selected but along with a temporal shift in bacterial cell size, this trend changed to negative selection during the experiment. Overall, our analysis of protistan food vacuole content indicated marked effects of flagellate prey selectivity on bacterioplankton community composition. 相似文献
53.
Stand age-related effects on soil respiration in a first rotation Sitka spruce chronosequence in central Ireland 总被引:7,自引:0,他引:7
GUSTAVO SAIZ KENNETH A. BYRNE† KLAUS BUTTERBACH-BAHL‡ RALF KIESE‡ VIOREL BLUJDEA§ EDWARD P. FARRELL 《Global Change Biology》2006,12(6):1007-1020
The effect of stand age on soil respiration and its components was studied in a first rotation Sitka spruce chronosequence composed of 10‐, 15‐, 31‐, and 47‐year‐old stands established on wet mineral gley in central Ireland. For each stand age, three forest stands with similar characteristics of soil type and site preparation were used. There were no significant differences in total soil respiration among sites of the same age, except for the case of a 15‐year‐old stand that had lower soil respiration rates due to its higher productivity. Soil respiration initially decreased with stand age, but levelled out in the older stands. The youngest stands had significantly higher respiration rates than more mature sites. Annual soil respiration rates were modelled by means of temperature‐derived functions. The average Q 10 value obtained treating all the stands together was 3.8. Annual soil respiration rates were 991, 686, 556, and 564 g C m?2 for the 10‐, 15‐, 31‐, and 47‐year‐old stands, respectively. We used the trenching approach to separate soil respiration components. Heterotrophic respiration paralleled soil organic carbon dynamics over the chronosequence, decreasing with stand age to slightly increase in the oldest stand as a result of accumulated aboveground litter and root inputs. Root respiration showed a decreasing trend with stand age, which was explained by a decrease in fine root biomass over the chronosequence, but not by nitrogen concentration of fine roots. The decrease in the relative contribution of autotrophic respiration to total soil CO2 efflux from 59.3% in the youngest stand to 49.7% in the oldest stand was explained by the higher activity of the root system in younger stands. Our results show that stand age should be considered if simple temperature‐based models to predict annual soil respiration in afforestation sites are to be used. 相似文献
54.
Reconciling Carbon-cycle Concepts, Terminology, and Methods 总被引:5,自引:1,他引:4
F. S. Chapin III G. M. Woodwell J. T. Randerson E. B. Rastetter G. M. Lovett D. D. Baldocchi D. A. Clark M. E. Harmon D. S. Schimel R. Valentini C. Wirth J. D. Aber J. J. Cole M. L. Goulden J. W. Harden M. Heimann R. W. Howarth P. A. Matson A. D. McGuire J. M. Melillo H. A. Mooney J. C. Neff R. A. Houghton M. L. Pace M. G. Ryan S. W. Running O. E. Sala W. H. Schlesinger E.-D. Schulze 《Ecosystems》2006,9(7):1041-1050
Recent projections of climatic change have focused a great deal of scientific and public attention on patterns of carbon (C)
cycling as well as its controls, particularly the factors that determine whether an ecosystem is a net source or sink of atmospheric
carbon dioxide (CO2). Net ecosystem production (NEP), a central concept in C-cycling research, has been used by scientists to represent two different
concepts. We propose that NEP be restricted to just one of its two original definitions—the imbalance between gross primary
production (GPP) and ecosystem respiration (ER). We further propose that a new term—net ecosystem carbon balance (NECB)—be
applied to the net rate of C accumulation in (or loss from [negative sign]) ecosystems. Net ecosystem carbon balance differs
from NEP when C fluxes other than C fixation and respiration occur, or when inorganic C enters or leaves in dissolved form.
These fluxes include the leaching loss or lateral transfer of C from the ecosystem; the emission of volatile organic C, methane,
and carbon monoxide; and the release of soot and CO2 from fire. Carbon fluxes in addition to NEP are particularly important determinants of NECB over long time scales. However,
even over short time scales, they are important in ecosystems such as streams, estuaries, wetlands, and cities. Recent technological
advances have led to a diversity of approaches to the measurement of C fluxes at different temporal and spatial scales. These
approaches frequently capture different components of NEP or NECB and can therefore be compared across scales only by carefully
specifying the fluxes included in the measurements. By explicitly identifying the fluxes that comprise NECB and other components
of the C cycle, such as net ecosystem exchange (NEE) and net biome production (NBP), we can provide a less ambiguous framework
for understanding and communicating recent changes in the global C cycle. 相似文献
55.
V. V. Maksimov E. V. Shchetinina O. V. Kraykivskaya E. A. Maksimova 《Microbiology》2006,75(6):653-657
The survival rate, metabolic activity, and ability for growth of microbial communities of Lake Baikal have been first studied after exposure to extremely low temperatures (freeze-thawing) for different lengths of time. It has been shown that short-term freezing (1–3 days) inhibits the growth and activity of microbial communities. The quantity of microorganisms increased after 7-and 15-day freezing. In the periods of maximums, the total number of microorganisms in the test samples was twice as high as in the control. It was established that after more prolonged freezing the microorganisms required more time after thawing to adapt to new conditions. In the variants with 7-and 15-day freezing, the activities of defrosted microbial communities were three or more times higher than in the control. The survival rate and activity of Baikal microorganisms after freeze-thawing confirms the fact that the Baikal microbial communities are highly resistant to this type of stress impact. 相似文献
56.
Growing Phototrophic Cells without Light 总被引:8,自引:0,他引:8
Many phototrophic microorganisms contain large quantities of high-value products such as n-3 polyunsaturated fatty acids and carotenoids but phototrophic growth is often slow due to light limitation. Some phototrophic
microorganisms can also grow on cheap organic substrate heterotrophically. Heterotrophic cultivation can be well controlled
and provides the possibility to achieve fast growth and high yield of valuable products on a large scale. Several strategies
have been investigated for cultivation of phototrophic microorganisms without light. These include trophic conversion of obligate
photoautotrophic microorganisms by genetic engineering, development of efficient cultivation systems and optimization of culture
conditions. This paper reviews recent advances in heterotrophic cultivation of phototrophic cells with an emphasis on microalgae. 相似文献
57.
Species composition, distribution, and the character of structural changes in the heterotrophic flagellate community were studied along environmental gradients in the Chernaya River estuary. There were 99 species and forms of heterotrophic flagellates, subdivided into three groups: prevalently marine species and euryhaline species preferring biotopes either of higher or decreased salinity. The heterotrophic flagellate community of the estuary was continuously divided into two distinct variants: (1) cenosis of halophilic species, prevalently of sea forms and euryhaline species preferring biotopes of increased salinity; (2) cenosis of halophobic species with prevalence of euryhaline forms gravitating to fresh biotopes. The arbitrary and indistinct boundary between the variants of the community ran at a salinity of 9–10‰. The response of estuarine communities of heterotrophic flagellates and infusorians to variation of abiotic factors was similar and differed from response of communities of microphyto-, meiozoo-and macrozoobenthos; this implied similarity of the response mechanism to environmental factors in organisms of one level of organization. 相似文献
58.
A culture of Thaumatomastix was isolated from a sediment sample collected in Oslofjorden and established as a monospecific strain (UIO286). Based on this culture, light and transmission electron microscopy and phylogenetic analyses were carried out. Thaumatomastix species are confined within the order Thaumatomonadida of the class Imbricatea and phylum Cercozoa. They are heterotrophic and their cell bodies are covered with silica scales. Observations of thin sections as well as whole mounts indicate that the morphology and ultrastructure of UIO286 is identical to T. salina, which was initially described from salt pools in Denmark. Detailed examination revealed some new features such as the presence of pseudopodia and silica deposition vesicles producing spine scales. The phylogeny presented here includes ribosomal DNA sequences from both imbricatean cultures and environmental samples. The 18S rDNA phylogenetic tree suggests that (i) Thaumatomastix is paraphyletic within the Thaumatomonadida clade, (ii) there is no close affinity between T. salina and other cultured and sequenced strains, but it is closely related to a sequence obtained from environmental DNA; we propose the present strain to serve as a reference culture of Thaumatomastix species and T. salina. Further, we discuss the distribution, habitats, and evolution of scale formation among euglyphids and thaumatomonads. 相似文献
59.
从驯化后的活性污泥中筛分、诱变出一株性能较好的异养硝化菌JZ1-1.经形态及生理生化特性分析,鉴定菌株JZ1-1为胶样菌属(Colloides sp.).分别考察了碳源、C/N、pH、溶解氧、温度和铵态氮初始浓度对JZ1-1硝化性能的影响.结果表明: 菌株对柠檬酸钠的利用较好;C/N为10~14、30 ℃、pH 6-9和转速150 r·min-1以上有利于铵态氮的降解;菌株对中高浓度铵态氮废水(100 mg·L-1≤铵态氮浓度≤500 mg·L-1)的降解效果显著.经5次继代培养,菌株的稳定性较好. 相似文献
60.
From 30 June to 24 September in 2003 ecosystem respiration (Re) in two alpine meadows on the Tibetan Plateau were measured using static chamber- and gas chromatography- (GC) based techniques. Simultaneously, plant removal treatments were set to partition Re into plant autotrophic respiration (Ra) and microbial heterotrophic respiration (Rh). Results indicated that Re had clear diurnal and seasonal variation patterns in both of the meadows. The seasonal variability of Re at both meadow sites was caused mainly by changes in Ra, rather than Rh. Moreover, atthe Kobresia humilis meadow site (K_site), Ra and Rh accounted for 54% and 46% of Re, respectively. While at the Potentilla fruticosa scrub meadow (P_site), the counterparts accounted for 61% and 39%, respectively. T test showed that there was significant difference in Re rates between the two meadows (t = 2.387, P = 0.022). However, no significant difference was found in Rh rates, whereas a significant difference was observed in Ra rates between the two meadows. Thus, the difference in Re rate between the two meadows was mainly attributed to plant autotrophic respirations. During the growing season, the two meadows showed relatively low Q10 values, suggesting that Re, especially Rh was not sensitive to temperature variation in the growing season. Additionally, Re and Rh at the K_site, as well as Rh at the Psite was negatively correlated with soil moisture, indicating that soil moisture would also play an important role in respirations. 相似文献