全文获取类型
收费全文 | 1578篇 |
免费 | 151篇 |
国内免费 | 50篇 |
出版年
2023年 | 13篇 |
2022年 | 12篇 |
2021年 | 14篇 |
2020年 | 22篇 |
2019年 | 43篇 |
2018年 | 49篇 |
2017年 | 40篇 |
2016年 | 32篇 |
2015年 | 49篇 |
2014年 | 71篇 |
2013年 | 95篇 |
2012年 | 57篇 |
2011年 | 89篇 |
2010年 | 60篇 |
2009年 | 62篇 |
2008年 | 59篇 |
2007年 | 85篇 |
2006年 | 63篇 |
2005年 | 88篇 |
2004年 | 72篇 |
2003年 | 51篇 |
2002年 | 53篇 |
2001年 | 44篇 |
2000年 | 36篇 |
1999年 | 38篇 |
1998年 | 44篇 |
1997年 | 43篇 |
1996年 | 31篇 |
1995年 | 34篇 |
1994年 | 24篇 |
1993年 | 30篇 |
1992年 | 19篇 |
1991年 | 17篇 |
1990年 | 25篇 |
1989年 | 18篇 |
1988年 | 13篇 |
1987年 | 11篇 |
1986年 | 12篇 |
1985年 | 19篇 |
1984年 | 19篇 |
1983年 | 15篇 |
1982年 | 24篇 |
1981年 | 14篇 |
1980年 | 9篇 |
1979年 | 17篇 |
1978年 | 10篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1974年 | 7篇 |
1973年 | 7篇 |
排序方式: 共有1779条查询结果,搜索用时 15 毫秒
11.
G. R. Findenegg 《Plant and Soil》1987,103(2):239-243
Seedlings of 14 species were grown for 14–28 days on nutrient solution with 6 mmol.l−1 NH4 as the sole nitrogen source. Solutions acidity was were kept constant at pH 4.0, 5.0, 6.0 and 7.0 by continuous titration
with diluted KOH. The following species were used: barley, maize, oats sorghum, yellow and white lupin, pea, soybean, carrot,
flax, castor-oil, spinach, sugarbeet and sunflower. Most plant species grew optimally at pH 6.0 with slight reductions at
pH 5.0. Growth of many species was severely inhibited at pH 4.0, but this inhibition was not observed with the legume and
cereal species. Yield depressions at pH 4.0 relative to pH 6.0 were well correlated with the respective relative decreases
of the K concentration in their roots (P<0.002). In the roots of two species (sunflower and flax) total N concentrations were also strongly reduced at pH 4.0. apparently,
the interactions between uptake of K, NH4 and H ions become the prevalent problem at suboptimal pH. At pH 7.0, yields were also considerably decreased, with the exception
of the lupines. At this pH, the roots of the growth inhibited plants were characterized by increased levels of total N and
free NH4. It is thought that the binding capacity of the roots for NH4 is an important factor in preventing NH4/NH3 toxicity at supraoptimal pH. 相似文献
12.
Interaction between nitrogen and photon flux density in birch seedlings at steady-state nutrition 总被引:7,自引:0,他引:7
Birch ( Betula pendula Roth.) was investigated under steady-state nutrition and growth at different incident photon flux densities (PFD) and different relative addition rates of nitrogen. PFD had a strong influence on the relative growth rate at optimum nutrition and on the nitrogen productivity (growth rate per unit of nitrogen) but little effect on the formal relationships between nitrogen and growth, i.e. PFD and nitrogen nutrition are orthogonal growth factors. At a given suboptimum nitrogen (the same distance from optimum), increased PFD increased the relative growth rate and, therefore, the relative uptake rate and the required relative addition rate in accordance with the theoretical equality between these three parameters at steady-state nutrition. Correspondingly, at a given suboptimum relative addition rate, increased PFD decreased nitrogen status (larger distance from optimum) at an unchanged relative growth rate. Nutrient uptake rate, dry matter content, and partitioning of biomass and nutrients are strongly influenced by nitrogen status. PFD influences these characteristics, but only to an extent corresponding to its effect on the nitrogen status. The influence of PDF on the relative growth rate at optimum and on nitrogen productivity is well described by hyperbolic relationships, similar to reported PFD/photosynthesis relationships. These expressions for plant growth as well as the productivities of leaf area and quantum appear to be valuable characteristics of plant responses to light and nutrition. Although the calculated PFD/growth relationships indicate saturation at high values of PFD, a more realistic estimate of PFD at which saturation occurs is about 30 mol m−2 day−1 , where the highest relative growth rate and nitrogen productivity were experimentally determined. No significant effect was observed because of day length differences between the present and previous experiments. 相似文献
13.
P. M. Abuja M. Schmuck I. Pilz P. Tomme M. Claeyssens H. Esterbauer 《European biophysics journal : EBJ》1988,15(6):339-342
Limited proteolysis (papain) of the cellobiohydrolase I (CBH I, 65 kDa) from Trichoderma reesei led to the seperation of two functional domains: a core protein (55 kDa) containing the active site, and a C-terminal glycopeptide (10 kDa) implicated in binding to the insoluble matrix (cellulose). The quaternary structures of the intact CBH I and its core in solution are now compared by small angle X-ray scattering (SAXS) measurements. The molecular parameters derived for the core (Rg=2.09 nm, Dmax=6.5 nm) and for the intact enzyme (Rg=4.27 nm, Dmax=18 nm) indicate very different shapes. The resulting models show a tadpole-like structure for the intact enzyme where the isotropic part coincides with the core protein and the flexible tail part should be identified with the C-terminal glycopeptide. Thus in this enzyme, functional differentiation is reflected in structural peculiarities.Abbreviations SAXS
small angle X-ray scattering
- SDS-PAGE
SDS-polyacrylamide gel electrophoresis
- IEF-PAG
polyacrylamide gel isoelectric focusing; cellobiohydrolase (CBH, 1,4--glucan cellobio hydrolase (E.C.3.2.1.91))
- Dmax
maximum diameter
- Rg
radius of gyration 相似文献
14.
Many soybean [Glycine max (L.) Merr.] genotypes that are grown in solution cultures are highly sensitive to the combination of both salinity and inorganic
phosphate (Pi) in the substrate. This effect has been observed on numerous occasions on plants grown in a saline medium that
contained a substantial amount of Ca (i.e., CaCl2/NaCl=0.5 on a molar basis). Because Ca is important in regulating ion transport and membrane permeability, solution culture
experiments were designed to examine the effects of various concentrations of Pi and ratios of CaCl2/NaCl (0 to 0.5 on a molar basis) at a constant osmotic potential (−0.34 MPa) on this adverse interaction. Four soybean cultivars
(‘Lee’, ‘Lee 74’ ‘Clark’ and ‘Clark 63’) were tested.
No adverse salinity x Pi interaction was found on Lee at any ratio and leaf P and Cl were maintained below 300 and 200 mmol
kg−1 dry wt, respectively. Clark, Clark 63 and Lee 74 soybean plants, on the other hand, were severely injured by solution salinity
(−0.34 MPa osmotic potential) when substrate Pi was ≥0.12 mM. Reduced substrate Ca did not intensify the salinity x Pi interaction. On the contrary, the onset of injury was hastened
and more severe with increased CaCl2/NaCl ratios in isotonic solutions. Shoot and root growth rates decreased as injury increased. Leaf P concentrations from
these cultivars grown in saline solutions with 0.12 mM Pi were excessive (>600 mmol kg−1 dry wt) compared with concentrations commonly found in soybean leaf tissue yet they were independent of the severity of injury.
Since leaf Cl increased wiht increased CaCl2/NaCl ratio, we suspect that the severity of foliar injury was related to the combined effects of excessive P and Cl within
the tissue. Lee 74, the only injured cultivar examined that excluded Cl from its leaves, was less sensitive than either Clark
cultivar and its injury was characteristically different. Other ion interactions were reported that may have played a role
in injury susceptibility. 相似文献
15.
The interactive effect of low P supply (0, 10, 20 and 40 M) and plant age on nodule number, mass and functioning (ureide analysis technique), vegetative growth and pod production were investigated in glasshouse-grown nodulated cowpea (Vigna unguiculata L.cv. Kausband) in sand culture. Compared with 40 M P, P stress (0 M P) or very low (10 M P) supply markedly impaired nodulation, allantoin and amino-N concentrations and weight of N solutes in xylem exudates. Consequently, P stress reduced top growth and pod yields by 48 and 90%, respectively. N solutes in xylem exudates and total plant N assayed by Kjeldahl technique (as estimates of N2 fixation) responded similarly to P supply. However, the relative ureide index [(ureide-N/ureide N+amino-N)×100] remained constant (99%), irrespective of P supply, indicating the plants' complete dependency on symbiosis for growth, without implying that growth was markedly increased by N2 fixation. Although P concentrations in plant tops, roots and nodules increased with P supply, N concentrations in these plant tissues were unaffected by P supply. The concentrations of N and P in the nodules were 2–2 1/2 times higher than in plant tops. P application interacted strongly with plant age, with the largest P effect evidently achieved at the early podding stage. The significance and implications of these results are discussed. 相似文献
16.
A field experiment was conducted on an Ultisol in Malaysia to assess changes in soil solution composition and their effects
on maize and groundnut yields, resulting from limestone and gypsum application. The results showed that soil solution Ca in
the lime treatment remained mainly in the zone of incorporation, but in the gypsum treatment some Ca moved into 15–30 cm zone.
Al3+ and AlSO4
+ were dominant Al species in the soil solution of nil treatment. Liming decreased Al3+ and AlSO4
+, but increased hydroxy-Al monomer activities. However, gypsum application resulted in an increase of AlSO4
+ activity and in a decrease of Al3+ activity.
Relative maize and groundnut yields were negatively correlated with Al3+, Al(OH)2+ and Alsum activities. Likewise, relative yields were negatively correlated with Al concentration and the Al concentration ratio and
positively correlated with soil solution Mg concentration and Ca/Al ratio. 相似文献
17.
J. Van Den Ende 《Plant and Soil》1991,133(1):65-74
The products of activities of calcium and sulphate were calculated for solutions of 75 glasshouse soils. The majority of these products was found to be higher than the solubility product of gypsum, thus indicating that these soil solutions were possibly supersaturated. In another investigation, soil solutions were examined to determine whether such high activity products could be really attributed to supersaturation. By means of ultracentrifuging of solutions of glasshouse soils, it could be established that the solutions were practically free of sulphate-bearing colloidal particles. Some solutions contained calcium-bearing colloidal particles, but the quantities of calcium contained in these particles were too small to substantially influence the calcium activity. Addition of gypsum crystals to soil solutions led to crystallization of so much calcium and sulphate that the products of the activities of calcium and sulphate dropped from values that can be listed as high to values approaching the solubility product of gypsum. The results obtained demonstrate the occurrence of supersaturation of soil solutions with respect to gypsum. It is further postulated that the presence of humic substances in the soil solution is responsible for this supersaturation. The possible occurrence of supersaturation with respect to gypsum in soils other than glasshouse soils is discussed. 相似文献
18.
19.
C L Wood M S O'Dorisio L M Vassalo W B Malarkey T M O'Dorisio 《Regulatory peptides》1985,12(3):237-248
The vasoactive intestinal polypeptide (VIP) receptor was characterized on the GH3 rat pituitary tumor cell line using competitive binding studies with peptides having sequence homology with VIP. Further studies investigated receptor coupling to the adenylate cyclase complex by measurement of cAMP levels. Finally, the molecular weight of the receptor was estimated by affinity labeling techniques. Studies using 125I-VIP and unlabeled competing peptides revealed a single class of high affinity binding sites with a dissociation constant (KD) of 17 +/- 2 nM (mean +/- S.E.M.) for VIP, 275 +/- 46 nM for peptide histidine isoleucine (PHI), and 1380 +/- 800 nM for human pancreatic growth hormone releasing factor (GHRF). VIP and PHI each stimulated intracellular cAMP accumulation in a dose-dependent manner; both peptides demonstrated synergism with forskolin. In contrast, GHRF neither stimulated accumulation of cAMP nor demonstrated synergism with forskolin. VIP plus PHI (1 microM each) caused no significant increase in cAMP over either VIP or PHI alone, implying that the two peptides act through the same receptor. Covalent crosslinking of 125I-VIP to its binding site using either disuccinimidyl suberate (DSS) or ethylene glycol bis(succinimidyl succinate) (EGS) was followed by SDS-PAGE and autoradiography. The result is consistent with an Mr 47 000 VIP-binding subunit comprising or being associated with the VIP receptor of GH3 pituitary tumor cells. 相似文献
20.
Steinar Solberg Tor Larsen Leif Jørgensen 《In vitro cellular & developmental biology. Plant》1985,21(11):612-616
Summary This study examined whether nonconfluent endothelial cell cultures reacted differently than confluent ones toward thrombin-stimulated
platelets or a heparinized salt solution. The adherence to the endothelial cell cultures of51Cr-labeled human platelets stimulated at different thrombin concentrations was studied. There was significantly higher adherence
of stimulated platelets to nonconfluent cultures compared with confluent ones. This was confirmed by scanning electron microscopy,
which also revealed a tendency for the platelets to adhere at the cell periphery. Electron microscopy also showed that thrombin-stimulated
platelets induced endothelial cell contraction. Part of the peripheral endothelial cell surface toward the bottom of the culture
dish was inverted, facing the lumen of the dish. This phenomenon was particularly seen in nonconfluent cultures. When51Cr-labeled endothelial cultures were incubated with a mildly injurious fluid as heparinized sodium acetate and 20% serum,
at 20° C for 30 min, the nonconfluent cultures showed significantly more cell detachment and release of51Cr than the confluent ones. We conclude that under the conditions of the present experiments there are differences in the
reactivity of confluent and nonconfluent endothelial cell cultures. These differences probably reflect biological dissimilarities.
In experiments where properties of cultured endothelium are studied, care should be taken that the degree of confluency is
standardized. 相似文献